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Effects of citric acid administration on femoral trabecular structures in ovariectomized mice

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Abstract

Objectives

To examine the effects of citric acid administration on trabecular structures.

Methods

Ovariectomized female ICR mice (n = 39), 8 weeks of age, were divided into four groups: ovariectomized control (OVX-control, n = 9, standard diet); citric acid (CIT, n = 10, citric acid 5 g/100 g diet); vitamin K2 (menaquinone 7; VK2, n = 10, vitamin K2 50 µg/100 g diet); and citric acid + vitamin K2 (CIT + VK2, n = 10, citric acid 5 g/100 g diet and vitamin K2 50 µg/100 g diet). After 12 weeks, the bone mass of the right femur was measured using peripheral quantitative computed tomography and the three-dimensional (3-D) trabecular structure of the right femur was assessed using microfocus computed tomography.

Results

The bone mineral density was significantly increased in the VK2 group versus the OVX-control group (P < 0.05). In the 3-D trabecular structure analysis, skeletal perimeter and number were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Skeletal separation, spacing, and trabecular bone pattern factor were significantly lower in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group. Almost all parameters in node–strut analysis were significantly greater in the CIT, VK2, and CIT + VK2 groups than in the OVX-control group.

Conclusions

These results suggest that citric acid improved trabecular structures. However, simultaneous administration of citric acid and vitamin K2 did not have any additional effect.

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References

  1. InstitutionalAuthorNameConsensus Development Conference (1993) ArticleTitleDiagnosis, prophylaxis, and treatment of osteoporosis Am J Med 94 646–50 Occurrence Handle10.1016/0002-9343(93)90218-E

    Article  Google Scholar 

  2. NIH Consensus Development Panel on Osteoporosis Prevention, Diagnosis and Therapy. JAMA 2001;285:785–95

    Google Scholar 

  3. MH Komulainen H Kroger MT Tropurainen AM Heikkinen E Alhava R Honkanen et al. (1998) ArticleTitleHRT and Vit. D in prevention of non-vertebral fractures in postmenopausal women Maturitas 31 45–54 Occurrence Handle10091204 Occurrence Handle10.1016/S0378-5122(98)00085-1

    Article  PubMed  Google Scholar 

  4. I Moriyama J Iwamoto T Takeda (2002) ArticleTitleComparative effects of intermittent administration of human parathyroid hormone (1–34) on cancellous and cortical bone loss in tail suspended and sciatic neurectomized young rats J Orthop Sci 7 379–85 Occurrence Handle12077665 Occurrence Handle10.1007/s007760200063

    Article  PubMed  Google Scholar 

  5. T Kameda K Miyazawa Y Mori T Yuasa M Shiokawa Y Nakamaru et al. (1996) ArticleTitleVitamin K2 inhibits osteoclastic bone resorption by inducing osteoclast apoptosis Biochem Biophys Res Commun 220 515–9 Occurrence Handle8607797 Occurrence Handle10.1006/bbrc.1996.0436

    Article  PubMed  Google Scholar 

  6. DM Black SR Cummings DB Karpf JA Cauley DE Thompson MC Nevitt et al. (1996) ArticleTitleRandomized trial of effect of alendronate on risk of fracture in women with existing vertebral fractures Lancet 348 1535–41 Occurrence Handle8950879 Occurrence Handle10.1016/S0140-6736(96)07088-2

    Article  PubMed  Google Scholar 

  7. NF Ray JK Chan M Thamer LJ Melton SuffixIII (1997) ArticleTitleMedical expenditures for the treatment of osteoporotic fractures in the United States in 1995: Report from the National Osteoporosis Foundation J Bone Miner Res 12 24–35 Occurrence Handle9240722 Occurrence Handle10.1359/jbmr.1997.12.1.24

    Article  PubMed  Google Scholar 

  8. B Lacour S Tardivel T Drueke (1997) ArticleTitleStimulation by citric acid of calcium and phosphorus bioavailability in rats fed a calcium-rich diet Miner Electrolyte Metab 23 79–87 Occurrence Handle9252973

    PubMed  Google Scholar 

  9. JA Harvey P Kenny J Poindexter CYC Pak (1990) ArticleTitleSuperior calcium absorption from calcium citrate than calcium carbonate using external forearm counting J Am Coll Nutr 9 583–7 Occurrence Handle2273192

    PubMed  Google Scholar 

  10. RP Heaney RR Recker CM Weaver (1990) ArticleTitleAbsorbability of calcium sources: the limited role of solubility Calcif Tissue Int 46 300–4 Occurrence Handle2110852 Occurrence Handle10.1007/BF02563819

    Article  PubMed  Google Scholar 

  11. MS Sheikh JA Maguire M Emet CA Santa Ana MJ Nicar LR Schiller et al. (1989) ArticleTitleReduction of dietary phosphorus absorption by phosphorus binders. A theoretical, in vitro and in vivo study J Clin Invest 83 66–73 Occurrence Handle2910921 Occurrence Handle10.1172/JCI113886

    Article  PubMed  Google Scholar 

  12. H Mehansho RL Kanerva GR Hudepohl KT Smith (1989) ArticleTitleCalcium bioavailability and iron-calcium interaction in orange juice J Am Coll Nutr 8 61–8 Occurrence Handle2926077

    PubMed  Google Scholar 

  13. T Fujikawa T Sakurai I Kashima (2004) ArticleTitleEffects of supplement containing vitamin K2 (MK7) and calcium phosphate (salmon bone powder) on the femur trabecular structure in ovariectomized mice: a study using three-dimensional imaging analysis Oral Radiol 20 37–43 Occurrence Handle10.1007/s11282-004-0005-0

    Article  Google Scholar 

  14. AM Parfitt (1988) ArticleTitleBone histomorphometry: standardization of nomenclature, symbols, and units (summary of proposed system) Bone 9 67–9 Occurrence Handle3377921 Occurrence Handle10.1016/8756-3282(88)90029-4

    Article  PubMed  Google Scholar 

  15. NL Fazzalari IH Parkinson (1996) ArticleTitleFractal dimension and architecture of trabecular bone J Pathol 178 100–5 Occurrence Handle8778308 Occurrence Handle10.1002/(SICI)1096-9896(199601)178:1<100::AID-PATH429>3.0.CO;2-K

    Article  PubMed  Google Scholar 

  16. M Hahn M Vogel M Pompesius-Kempa G Delling (1992) ArticleTitleTrabecular bone pattern factor – a new parameter for simple quantification of bone microarchitecture Bone 13 327–30 Occurrence Handle1389573 Occurrence Handle10.1016/8756-3282(92)90078-B

    Article  PubMed  Google Scholar 

  17. NJ Garrahan RW Mellish JE Compston (1986) ArticleTitleA new method for the two-dimensional analysis of bone structure in human iliac crest biopsies J Microsc 142 341–9 Occurrence Handle3735417

    PubMed  Google Scholar 

  18. JA Kanis O Johnell C De Laet B Jonsson A Oden AK Ogelsby (2002) ArticleTitleInternational variations in hip fracture probabilities: implications for risk assessment J Bone Miner Res 17 1237–44 Occurrence Handle12096837 Occurrence Handle10.1359/jbmr.2002.17.7.1237

    Article  PubMed  Google Scholar 

  19. Y Akedo T Hosoi S Inoue A Ikegami Y Mizuno M Kaneki et al. (1992) ArticleTitleVitamin K2 modulates proliferation and function of osteoblastic cells in vitro Biochem Biophys Res Commun 187 814–20 Occurrence Handle1530637 Occurrence Handle10.1016/0006-291X(92)91269-V

    Article  PubMed  Google Scholar 

  20. AG Need HA Morris M Horowitz E Scopacasa BE Nordin (1998) ArticleTitleIntestinal calcium absorption in men with spinal osteoporosis Clinical Endocrinology 48 163–8 Occurrence Handle9579227 Occurrence Handle10.1046/j.1365-2265.1998.3681181.x

    Article  PubMed  Google Scholar 

  21. M Yamaguchi H Taguchi YH Gao A Igarashi Y Tsukamoto (1999) ArticleTitleEffect of vitamin K2 (menaquinone-7) in fermented soybean (natto) on bone loss in ovariectomized rats J Bone Miner Metab 17 23–9 Occurrence Handle10084398 Occurrence Handle10.1007/s007740050059

    Article  PubMed  Google Scholar 

  22. MM Tabb A Sun C Zhou F Grün J Errandi K Romero et al. (2003) ArticleTitleVitamin K2 regulation of bone homeostasis is mediated by the steroid and xenobiotic receptor SXR J Bio Chem 278 43919–27 Occurrence Handle10.1074/jbc.M303136200

    Article  Google Scholar 

  23. B Ettinger JP Bilezikian (2002) ArticleTitleFor osteoporosis, are two antiresorptive drugs better than one? J Clin Endocrinol Metab 87 983–4 Occurrence Handle11889148 Occurrence Handle10.1210/jc.87.3.983

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Radiology, Department of Maxillofacial Diagnostic Science, Kanagawa Dental College, 82 Inaoka, Yokosuka, 238-8580, Japan

    Masayasu Miyake, Yusuke Kozai, Takashi Sakurai & Isamu Kashima

Authors
  1. Masayasu Miyake
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  2. Yusuke Kozai
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  3. Takashi Sakurai
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  4. Isamu Kashima
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Corresponding author

Correspondence to Takashi Sakurai.

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Miyake, M., Kozai, Y., Sakurai, T. et al. Effects of citric acid administration on femoral trabecular structures in ovariectomized mice. Oral Radiol 23, 34–41 (2007). https://doi.org/10.1007/s11282-007-0063-1

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  • DOI: https://doi.org/10.1007/s11282-007-0063-1

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