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Clamping renal artery alone produces less ischemic damage compared to clamping renal artery and vein together in two animal models: near-infrared tissue oximetry and quantitation of 8-isoprostane levels

  • Urology - Original Paper
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Abstract

Purpose

To investigate renal ischemia injury during renal hilar clamping (artery alone versus clamping artery/vein together) by evaluating ischemic damage via two different modalities in animal models—near-infrared tissue oximetry and 8-isoprostane levels.

Methods

Near-infrared renal oximetry measurements of Yorkshire swines (n = 4; 8 renal units) subject to hilar clamping were obtained at baseline, during warm ischemia (15- and 30-min trials) and after unclamping. Quantitation of 8-isoprostane levels is the second technique of quantitating interstitial fluid collected from a dialysis catheter placed through renal parenchyma of male Sprague–Dawley rats (n = 50) subject to hilar clamping during preclamp, clamp (either 15 or 30 min of hilum clamping), and post-clamp.

Results

N ear-infrared tissue oximetry. In the 15-min trial, oxygen saturation decreased 6× faster with artery alone compared to artery/vein clamped together. In the 30-min trial, the decrease was 5× faster. Recovery of oxygen saturation with only artery clamped occurred more than 2× faster in the 15- and 30-min periods. Isoprostane. For 15-min clamp times, 8-isoprostane levels in the artery alone group demonstrated a 1.54 decrease in the artery clamped alone group (p = 0.006) versus artery/vein together: preclamp (11.47 and 11.63 pg/mL/g), clamp (14.61 and 17.70 pg/mL/g), and post-clamp (14.26 and 22.04 pg/mL/g).

Conclusions

Renal ischemia injury from clamping the renal artery alone was significantly less than clamping artery/vein together demonstrated in two different techniques. Recovery of oxygen saturation was twofold faster, and mean post-clamp 8-isoprostane levels demonstrated a 1.54-fold decrease with clamping renal artery alone compared to clamping artery/vein together.

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Conflict of interest

The authors declare that that they have no conflict of interest on this study.

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Authors and Affiliations

  1. Department of Urology, Tulane University School of Medicine, 1430 Tulane Avenue, SL-42, Room 3522, New Orleans, LA, 70112, USA

    J. L. Colli, Z. Wang, N. Johnsen, L. Grossman & B. R. Lee

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  1. J. L. Colli
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  2. Z. Wang
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  3. N. Johnsen
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  4. L. Grossman
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  5. B. R. Lee
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Correspondence to J. L. Colli.

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Colli, J.L., Wang, Z., Johnsen, N. et al. Clamping renal artery alone produces less ischemic damage compared to clamping renal artery and vein together in two animal models: near-infrared tissue oximetry and quantitation of 8-isoprostane levels. Int Urol Nephrol 45, 421–428 (2013). https://doi.org/10.1007/s11255-012-0297-7

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  • DOI: https://doi.org/10.1007/s11255-012-0297-7

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