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NF-YB Family Genes Identified in a Poplar Genome-wide Analysis and Expressed in Populus euphratica Are Responsive to Drought Stress

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Abstract

Some members of the nuclear transcription factor Y subunit B (NF-YB) have been newly reported to regulate a variety of functions in annual plant development and drought tolerance. The overall goal of this study was to identify and characterize the NF-YB genes in the model tree Populus trichocarpa, with the expectation of determining which orthologs in P. euphratica, naturally distributed in semiarid regions, were involved in drought response. A total of 20 NF-YB genes in the P. trichocarpa genome were identified and annotated. The examination on their phylogenetic relationships and ortholog predictions showed that Populus has an expanded set of NF-YB genes within unknown functional groups in comparison to Arabidopsis. Four members of the PeNF-YB gene family in P. euphratica were found to be up-regulated in expression during PEG-6000 (polyethylene glycol 6000) drought treatment based on RT-qPCR analyses. The increased knowledge on the phylogenetic relationships, predicted orthologs, and expression patterns of the poplar NF-YB genes during drought stress can be expected to promote the future study of woody plants.

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Acknowledgments

The research was supported by grants from the Ministry of Science and Technology of China (2011BAD38B01, 2009CB119101), and the National Natural Science Foundation of China (30972339, 31070597, 30730077).

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Authors and Affiliations

  1. College of Biological Sciences and Technology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China

    Dong-Hui Yan, Xinli Xia & Weilun Yin

  2. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China

    Dong-Hui Yan

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  1. Dong-Hui Yan
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  2. Xinli Xia
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Correspondence to Weilun Yin.

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ESM 1

Primers designed based on the 20 NF-YB genes in Populus genome for amplicon of P. euphratica NF-YB genes by RT-qPCR. (XLSX 14 kb)

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Yan, DH., Xia, X. & Yin, W. NF-YB Family Genes Identified in a Poplar Genome-wide Analysis and Expressed in Populus euphratica Are Responsive to Drought Stress. Plant Mol Biol Rep 31, 363–370 (2013). https://doi.org/10.1007/s11105-012-0508-5

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