Abstract
Magainins are a class of antimicrobial peptides isolated from skin secretions of the African clawed frog Xenopus laevis. MSI-99 is a synthesized magainin II analogue with high inhibitory effects to a wide spectrum of microbial organisms, including bacteria, fungi and viruses. To verify the resistance conferred by the MSI-99m gene (a MSI-99 synthetic gene with codon usage adapted for expression in potato) to potato pathogens and to generate multi-resistant potato materials, we transferred the MSI-99m gene into potato plants using Agrobacterium-mediated transformation. PCR and Southern blot analyses of eight kanamycin-resistant plants showed that MSI-99m gene was present and expressed in five independent transgenic lines. These five transgenic plants exhibited enhanced resistance to Phytophthora infestans and Ralstonia solanacearum. The results demonstrate that the MSI-99m gene can be used to potentially improve potato disease resistance genetically.
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Acknowledgments
This study was supported financially by grants from the Jiangsu Agricultural Science and Technology Innovation Fund (NO. cx (11)1020), the National ‘863’ High-tech Program (NO. 2010AA10A108), and the National Pear Industry Technology System (NO. CARS-29). We gratefully acknowledge the Department of Plant Pathology of Nanjing Agricultural University, for providing pathogens Phytophthora infestans and Ralstonia solanacearum. We specially thank Professor Wang Yuanchao for his assistance in techniques.
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Hong, Yb., Liu, Sp., Zhu, Yp. et al. Expression of the MSI-99m Gene in Transgenic Potato Plants Confers Resistance to Phytophthora infestans and Ralstonia solanacearum . Plant Mol Biol Rep 31, 418–424 (2013). https://doi.org/10.1007/s11105-012-0503-x
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DOI: https://doi.org/10.1007/s11105-012-0503-x