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Incidence of dsRNA Mycoviruses in a Collection of Aspergillus fumigatus Isolates

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Abstract

A collection of clinical and environmental isolates of the opportunistic human pathogen, Aspergillus fumigatus, were screened for the presence of mycoviruses and 6.6 % of 366 isolates contained dsRNA segments ranging in size from ~1.0 to 4.0 kbp. The dsRNAs were categorised into three different groups comprising bipartite dsRNAs, quadripartite dsRNAs, representative isolates of which have both been sequenced, and an uncharacterised mycovirus, whose genome apparently consists of four dsRNAs 1–2.5 kbp in size. Here, we describe dsRNA incidence in the A. fumigatus isolates examined, their provenance and also note that on occasion individual isolates were infected with two groups of different dsRNAs.

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Acknowledgments

We gratefully acknowledge the support of the Chelsea & Westminster Hospital Special Trustees through Dr David Shanson and thank Dr Christopher Thornton at Exeter University for a collection of environmental isolates of A. fumigatus. We also thank Dr Emilise Diaz for technical assistance and HEC of Pakistan for supporting the PhD studies of Atif Jamal and Muhammad Faraz Bhatti.

Conflict of interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, SW7 2AZ, UK

    Robert H. A. Coutts

  2. Atta-ur-Rehman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Kasmir Highway, Islamabad, 44000, Pakistan

    Muhammad F. Bhatti

  3. National Agricultural Research Centre, Park Road, Islamabad, 45500, Pakistan

    Atif Jamal

  4. Division of Investigative Science, Faculty of Medicine, Imperial College London, Imperial College Road, London, SW7 2AZ, UK

    Elaine M. Bignell

  5. Division of Infectious Diseases, Faculty of Medicine, Hammersmith Hospital, Du Cane Road, London, W12 0HS, UK

    Michael A. Petrou

Authors
  1. Muhammad F. Bhatti
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  2. Atif Jamal
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  3. Elaine M. Bignell
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  4. Michael A. Petrou
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  5. Robert H. A. Coutts
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Corresponding author

Correspondence to Robert H. A. Coutts.

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Bhatti, M.F., Jamal, A., Bignell, E.M. et al. Incidence of dsRNA Mycoviruses in a Collection of Aspergillus fumigatus Isolates. Mycopathologia 174, 323–326 (2012). https://doi.org/10.1007/s11046-012-9556-5

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  • DOI: https://doi.org/10.1007/s11046-012-9556-5

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