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Influence of the relative humidity on aminosilane molecular grafting properties

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Abstract

Functional coatings with amino groups are used in a wide range of biochemistry-related applications. A technological platform that takes advantage of the affinity between amino-functionalized coatings and biomolecules is the DNA microarray methodology. Reliability and reproducibility of the microarray data strongly depend on the quality of the substrate; therefore, a proper awareness of how the storage conditions affect the amino-functionalized coatings is necessary. In this work we have studied the influence of different relative humidity levels on amino-methyl-silane coatings prepared via sol–gel methodology. Drops of a buffer solution containing a luminescent dye have been deposited (or spotted) on the coating; the dye molecules react with the amino-groups and leave circular luminescent marks (spots) on the substrate surface. Shape and luminescence of the spots, as well as background signals, have been monitored using a microarray laser scanner. With the proposed protocol we have measured the changes of these variables due to different storage conditions. FT-IR measurements have been performed to investigate the related chemistry changes.

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Acknowledgments

This work has been partially supported by the Fondazione di Venezia within the DICE project. Anna Meneghello is acknowledged for helpful discussions.

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Authors and Affiliations

  1. Chemistry and Industrial Chemistry Department, Università di Pisa, 56126, Pisa, Italy

    Fabio Lisi

  2. Division of Materials Science and Engineering, CSIRO, Private Bag 33, Clayton South MDC, VIC, 3169, Australia

    Fabio Lisi, Dario Buso, Anita J. Hill & Paolo Falcaro

  3. Associazione CIVEN—Nano Fabrication Facility, Via delle Industrie 9, 30175, Marghera, Venice, Italy

    Davide Carta, Stefano Costacurta & Paolo Falcaro

  4. Department of Statistics, Università Ca’ Foscari di Venezia, San Giobbe, Cannareggio 873, 30121, Venice, Italy

    Laura Villanova & Irene Poli

  5. European Centre for Living Technology, Ca’ Minich, San Marco 2940, 30124, Venice, Italy

    Laura Villanova & Irene Poli

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  1. Fabio Lisi
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  2. Davide Carta
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  3. Laura Villanova
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  4. Irene Poli
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  5. Dario Buso
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  6. Stefano Costacurta
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  8. Paolo Falcaro
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Correspondence to Paolo Falcaro.

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Lisi, F., Carta, D., Villanova, L. et al. Influence of the relative humidity on aminosilane molecular grafting properties. J Sol-Gel Sci Technol 60, 246–253 (2011). https://doi.org/10.1007/s10971-011-2492-x

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  • DOI: https://doi.org/10.1007/s10971-011-2492-x

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