Skip to main content
SpringerLink
Log in

Visualization of how light changes affect ion movement in rice plants using a real-time radioisotope imaging system

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

We developed a real-time radioisotope imaging system (RRIS) technique that can nondestructively visualize the element absorption and transport process in plants, using not only positron emitters but also commercially available radioisotopes. In this study, we applied RRIS to analyze light effects on ion movement in rice plants. As tracers, 28Mg, 32P, and 45Ca were used. During the first 5 h, dark/light cycle of 3/7 min were set up; the RRIS needs dark conditions during capturing. When the light was ceased 5 h after supplying each tracer, 32P transport from root to shoot decreased immediately. In contrast, 28Mg and 45Ca transport did not change with light conditions. These results suggest that the P transport is dependent on water flow, whereas Mg and Ca transport are independent of water flow.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Lazar T, Taiz L, Zeiger E (2003) Plant physiology. 3rd edn. Ann. Bot. Doi: 10.1093/aob/mcg079

  2. Sakurai-Ishikawa J, Murai-Hatano M, Hayashi H, Ahamed A, Fukushi K, Matsumoto T, Kitagawa Y (2011) Transpiration from shoots triggers diurnal changes in root aquaporin expression. Plant Cell Environ. doi:10.1111/j.1365-3040.2011.02313.x

    Google Scholar 

  3. Fageria NK, Baligar VC, Clark R (2006) Physiology of crop production. CRC Press, Boca Raton

    Google Scholar 

  4. Orlich G (1998) Analysis of the driving forces of phloem transport in Ricinus seedlings: sucrose export and volume flow are determined by the source. Planta. doi:10.1007/s004250050399

    Google Scholar 

  5. Biddulph O, Cory R (1957) An analysis of translocation in the phloem of the bean plant using Tho, P(32), And C(14). Plant Physiol 32:608–619

    Article  CAS  Google Scholar 

  6. Van Bel AJE (1976) Different mass transfer rates of labeled sugar and tritiated water in xylem vessels and their dependency on metabolism. Plant Physiol 57:911–914

    Article  Google Scholar 

  7. Ohya T, Tanoi K, Hamada Y, Okabe H, Rai H, Hojo J, Suzuki K, Nakanishi T (2008) An analysis of long-distance water transport in the soybean stem using (H2O)-O-15. Plant Cell Physiol 49:718–729

    Article  CAS  Google Scholar 

  8. Tanoi K, Hojo J, Nishioka M, Nakanishi T, Suzuki K (2005) New technique to trace [O-15]water uptake in a living plant with an imaging plate and a BGO detector system. J Radioanal Nucl Chem 263:547–552

    Article  CAS  Google Scholar 

  9. Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM (2016) Visualization of mineral elements uptake and the dynamics of photosynthates in arabidopsis by a newly developed real-time radioisotope imaging system (RRIS). Plant Cell Physiol. doi:10.1093/pcp/pcw056

    Google Scholar 

  10. Marschner H (1995) Mineral nutrition of higher plants. Academic Press, New York

    Google Scholar 

  11. Kobayashi NI, Sugita R, Nobori T, Tanoi K, Nakanishi TM (2016) Tracer experiment using 42K+ and 137Cs+ revealed the different transport rates of potassium and caesium within rice roots. Funct Plant Biol. doi:10.1071/FP15245

    Google Scholar 

  12. Tanoi K, Kobayashi NI, Saito T, Iwata N, Hirose A, Ohmae Y, Iwata R, Suzuki H, Nakanishi TM (2013) Application of 28 Mg to the kinetic study of Mg uptake by rice plants. J Radioanal Nucl. doi:10.1007/s10967-012-2219-7

    Google Scholar 

  13. Sugita R, Kobayashi NI, Hirose A, Tanoi K, Nakanishi TM (2014) Evaluation of in vivo detection properties of 22Na, 65Zn, 86Rb, 109Cd and 137Cs in plant tissues using real-time radioisotope imaging system. Phys Med Biol. doi:10.1088/0031-9155/59/4/837

    Google Scholar 

  14. Kanno S, Yamawaki M, Ishibashi H, Kobayashi NI, Hirose A, Tanoi K, Nussaume L, Nakanishi TM (2012) Development of real-time radioisotope imaging systems for plant nutrient uptake studies. Philos Trans R Soc B. doi:10.1098/rstb.2011.0229

    Google Scholar 

  15. Hirose A, Yamawaki M, Kanno S, Igarashi S, Sugita R, Ohmae Y, Tanoi K, Nakanishi TM (2013) Development of a C-14 detectable real-time radioisotope imaging system for plants under intermittent light environment. J Radioanal Nucl. doi:10.1007/s10967-012-2130-2

    Google Scholar 

  16. Karley AJ, White PJ (2009) Moving cationic minerals to edible tissues: potassium, magnesium, calcium. Curr Opin Plant Biol 12:291–298

    Article  CAS  Google Scholar 

  17. Biddulph O, Nakayama FS, Cory R (1961) Transpiration stream & ascension of calcium. Plant Physiol 36:429–436

    Article  CAS  Google Scholar 

  18. Matsuo T, Kumazawa K, Ishii R, Ishihara K, Hirata H (1995) Science of the rice plant, vol 2. Physiology, Food and Agriculture Policy Research Center, Tokyo

    Google Scholar 

Download references

Acknowledgements

This work was partly supported by JSPS KAKENHI Grant Number 15H02469 to T. M. Nakanishi, and 15k18761 to R. Sugita; the Japan Science and Technology Agency (JST) [PRESTO] (# 15665950) to K. Tanoi.

Author information

Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113–8657, Japan

    Ryohei Sugita, Natsuko I. Kobayashi, Atsushi. Hirose, Keitaro Tanoi & Tomoko M. Nakanishi

  2. Cyclotron and Radioisotope Center (CYRIC), Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan

    Ren Iwata

  3. National Institute of Radiological Sciences, 4-9-1, Anagawa, Inage-ku, Chiba-shi, Chiba, 263-8555, Japan

    Hisashi Suzuki

  4. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Keitaro Tanoi

Authors
  1. Ryohei Sugita
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Natsuko I. Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Atsushi. Hirose
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ren Iwata
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hisashi Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Keitaro Tanoi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tomoko M. Nakanishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Keitaro Tanoi.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sugita, R., Kobayashi, N.I., Hirose, A. et al. Visualization of how light changes affect ion movement in rice plants using a real-time radioisotope imaging system. J Radioanal Nucl Chem 312, 717–723 (2017). https://doi.org/10.1007/s10967-017-5193-2

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-017-5193-2

Keywords

Search

Navigation