Abstract
The hydroxyapatite (HAp) powder preparation process was optimized to fabricate inositol phosphate-HAp (IP6-HAp) cement with enhanced mechanical properties. Starting HAp powders were synthesized via a wet chemical process. The effect of the powder preparation process on the morphology, crystallinity, median particle size, and specific surface area (SSA) of the cement powders was examined, together with the mechanical properties of the resulting cement specimens. The smallest crystallite and median particle sizes, and the highest SSA were obtained from ball-milling of as-synthesized HAp powder under wet conditions and then freeze-drying. IP6-HAp cement fabricated with this powder had a maximum compressive strength of 23.1 ± 2.1 MPa. In vivo histological studies using rabbit models revealed that the IP6-HAp cements were directly in contact with newly formed and host bones. Thus, the present chelate-setting HAp cement is promising for application as a novel paste-like artificial bone.
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References
LeGeros RZ, LeGeros JP. Dense hydroxyapatite. In: Hench LL, Wilson J, editors. An introduction to bioceramics. Singapore: World Scientific; 1993. p. 139–80.
Neo M, Nakamura T, Ohtsuki C, Kokubo T, Yamamuro T. Apatite formation on three kinds of bioactive material at an early stage in vivo: a comparative study by transmission electron microscopy. J Biomed Mater Res. 1993;27:999–1006.
Kawata M, Uchida H, Itatani K, Okada I, Koda S, Aizawa M. Development of porous ceramics with well-controlled porosities and pore sizes from apatite fibers and their evaluations. J Mater Sci Mater Med. 2004;15:817–23.
Oonishi H, Hench LL, Wilson J, Sugihara F, Tsuji E, Kushitani S, Iwaki H. Comparative bone growth behavior in granules of bioceramic materials of various sizes. J Biomed Mater Res. 1999;44:31–43.
Monma H, Kanazawa T. The hydration of α-tricalcium phosphate. Yogyo-Kyokai-Shi. 1976;84:209–13.
Brown WE, Chow LC. Dental restorative cement pastes. US Patent No. US4518430; 1985.
Chow LC, Takagi S. Calcium phosphate hydroxyapatite precursor and methods for making and using the same. US Patent No. US5522893; 1996.
Miyamoto Y, Ishikawa K, Takechi M, Toh T, Yuasa T, Nagayama M, Suzuki K. Histological and compositional evaluations of three types of calcium phosphate cements when implanted in subcutaneous tissue immediately after mixing. J Biomed Mater Res. 1999;48:36–42.
Aizawa M, Haruta Y, Okada I. Development of novel cement processing using hydroxyapatite particles modified with inositol phosphate. Arch BioCeram Res. 2003;3:134–8.
Horiguchi Y, Yoshikawa A, Oribe K, Aizawa M. Fabrication of chelate-setting hydroxyapatite cements from four kinds of commercially-available powder with various shape and crystallinity and their mechanical property. J Ceram Soc Jpn. 2008;116:50–5.
Dao TH. Polyvalent cation effects on myo-inositol hexakis dihydrogenphosphate enzymatic dephosphorylation in dairy wastewater. J Environ Qual. 2003;32:694–701.
Alcázar-Román AR, Bolger TA, Wente SR. Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle1. J Biol Chem. 2010;285:16683–92.
Martin CJ, Evans WJ. Phytic acid-metal ion interactions. II. The effect of pH on Ca(II) binding. J Inorg Biochem. 1986;27:17–30.
Hirabayashi S, Hirasawa T, Kohno A, Usui H. Studies on primers combined chelating agent with metallic ion for tooth-bonding I. Effects of primer combined phytic acid and tin(II) fluoride. J Jpn Soc Dent Mater Dev. 1993;12:435–44.
Mosekilde L, Mosekilde L. Normal vertebral body size and compressive strength: relations to age and to vertebral and iliac trabecular bone compressive strength. Bone. 1986;7:207–12.
Akao M, Aoki H, Kato K. Mechanical properties of sintered hydroxyapatite for prosthetic applications. J Mater Sci. 1981;16:809–12.
Lu H, Qu Z, Zhou Y. Preparation and mechanical properties of dense polycrystalline hydroxyapatite through freeze-drying. J Mater Sci Mater Med. 1998;9:583–7.
Hansen NM, Felix R, Bisaz S, Fleisch H. Aggregation of hydroxyapatite crystals. Biochim Biophys Acta. 1976;451:549–59.
Marković S, Veselinović L, Lukić MJ, Karanović L, Bračko I, Ignjatović N, Uskoković D. Synthetical bone-like and biological hydroxyapatites: a comparative study of crystal structure and morphology. Biomed Mater. 2011;6:045005.
Kannan S, Rocha JHG, Ventura JMG, Lemos AF, Ferreira JMF. Effect of Ca/P ratio of precursors on the formation of different calcium apatitic ceramics–an X-ray diffraction study. Scr Mater. 2005;53:1259–62.
Ganesan K, Epple M. Calcium phosphate nanoparticles as nuclei for the preparation of colloidal calcium phytate. New J Chem. 2008;32:1326–30.
Ishikawa K, Asaoka K. Estimation of ideal mechanical strength and critical porosity of calcium phosphate cement. J Biomed Mater Res. 1995;29:1537–43.
Duckworth W. Discussion of Ryshkewitch paper by Winston Duckworth. J Am Ceram Soc. 1953;36:68.
Barralet JE, Gaunt T, Wright AJ, Gibson IR, Knowles JC. Effect of porosity reduction by compaction on compressive strength and microstructure of calcium phosphate cement. J Biomed Mater Res. 2002;63:1–9.
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The present study was supported in part by a Grant for Practical Application of University R&D Results under the Matching Fund Method from the New Energy and Industrial Technology Development Organization (NEDO), Japan.
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Konishi, T., Horiguchi, Y., Mizumoto, M. et al. Novel chelate-setting calcium-phosphate cements fabricated with wet-synthesized hydroxyapatite powder. J Mater Sci: Mater Med 24, 611–621 (2013). https://doi.org/10.1007/s10856-012-4834-9
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DOI: https://doi.org/10.1007/s10856-012-4834-9