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Comparison of vitrification and slow cooling for umbilical tissues

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Abstract

The tissue cryopreservation maintains the cellular metabolism in a quiescence state and makes the conservation possible for an indefinite period of time. The choice of an appropriate cryopreservation protocol is essential for maintenance of cryopreserved tissue banks. This study evaluated 10 samples of umbilical cord, from which small fragments of tissue (Wharton’s jelly and cord lining membrane) were subjected to two protocols of cryopreservation: slow cooling and vitrification. The samples were frozen for a period of time ranging from 5 to 78 days. The efficiency of cryopreservation was evaluated by testing cell viability, histological analysis, cell culture, cytogenetic analysis and comparison with the results of the fresh samples. The results showed that the slow cooling protocol was more efficient than the vitrification for cryopreservation of umbilical cord tissue, because it has caused fewer changes in the structure of tissue (edema and degeneration of the epithelium) and, despite the significant decrease cell viability compared to fresh samples, the ability of cell proliferation in vitro was preserved in most samples. In conclusion, this study showed that it is possible to cryopreserve small fragments of tissue from the umbilical cord and, to obtain viable cells capable of proliferation in vitro after thawing, contributing to the creation of a frozen tissue bank.

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Authors and Affiliations

  1. Laboratory of Animal and Human Genetics, Department of General Biology, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Lilian Da-Croce & Ana Lúcia Brunialti Godard

  2. Sector of Cytogenetics, Department of Genetics, Hermes Pardini Institute, Belo Horizonte, Minas Gerais, Brazil

    Lilian Da-Croce, Greicy Helen Ribeiro Gambarini-Paiva & Patrícia Caroline Angelo

  3. Department of Pathological Anatomy and Legal Medicine, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Eduardo Alves Bambirra

  4. Department of Pathological Anatomy, Hermes Pardini Institute, Belo Horizonte, Minas Gerais, Brazil

    Eduardo Alves Bambirra

  5. Department of Ginecology and Obstetrics, Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Antônio Carlos Vieira Cabral

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  1. Lilian Da-Croce
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  2. Greicy Helen Ribeiro Gambarini-Paiva
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  3. Patrícia Caroline Angelo
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  4. Eduardo Alves Bambirra
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  6. Ana Lúcia Brunialti Godard
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Correspondence to Greicy Helen Ribeiro Gambarini-Paiva.

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Da-Croce, L., Gambarini-Paiva, G.H.R., Angelo, P.C. et al. Comparison of vitrification and slow cooling for umbilical tissues. Cell Tissue Bank 14, 65–76 (2013). https://doi.org/10.1007/s10561-012-9301-9

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  • DOI: https://doi.org/10.1007/s10561-012-9301-9

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