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Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells

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Abstract

This study investigates the feasibility of processed human amnion (HAM) as a substrate for chondrogenic differentiation of mesenchymal stem cells (MSCs). HAM preparations processed by air drying (AD) and freeze drying (FD) underwent histological examination and MSC seeding in chondrogenic medium for 15 days. Monolayer cultures were used as control for chondrogenic differentiation and HAMs without cell seeding were used as negative control. Qualitative observations were made using scanning electron microscopy analysis and quantitative analyses were based on the sulfated glycosaminoglycans (GAG) assays performed on day 1 and day 15. Histological examination of HAM substrates before seeding revealed a smooth surface in AD substrates, while the FD substrates exhibited a porous surface. Cell attachment to AD and FD substrates on day 15 was qualitatively comparable. GAG were significantly highly expressed in cells seeded on FD HAM substrates. This study indicates that processed HAM is a potentially valuable material as a cell-carrier for MSC differentiation.

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Acknowledgments

This study was supported by E-Science grant from Ministry of Science, Technology and Innovation (12-02-03-2017) and FRGS FQQ002/2007A.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Sik-Loo Tan, Cheh-Chin Tai & T. Kamarul

  2. Department of Obstetric and Gynaecology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Sofiah Sulaiman

  3. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Belinda Pingguan-Murphy

  4. School of Medicine and Health Sciences, Monash University, Sunway Campus, 46150, Subang Jaya, Selangor, Malaysia

    L. Selvaratnam

Authors
  1. Sik-Loo Tan
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  2. Sofiah Sulaiman
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  3. Belinda Pingguan-Murphy
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  4. L. Selvaratnam
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  5. Cheh-Chin Tai
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  6. T. Kamarul
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Corresponding author

Correspondence to T. Kamarul.

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Tan, SL., Sulaiman, S., Pingguan-Murphy, B. et al. Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells. Cell Tissue Bank 12, 59–70 (2011). https://doi.org/10.1007/s10561-009-9164-x

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  • DOI: https://doi.org/10.1007/s10561-009-9164-x

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