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Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism

  • Original Paper
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Biologia Plantarum

Abstract

Identifying important regulative elements and pathways related to dwarfism in cotton is a major challenge in cotton breeding. Isobaric tags for relative and absolute quantitation-based proteomics of stem terminal buds from upland cotton (Gossypium hirsutum L.) dwarf line LA-1 and high near-isogenic line LH-1 was performed. Moreover, transcriptional expression of differentially-expressed proteins (DEPs) belonging to phytohormone signal transduction, hormone biosynthesis, and ubiquitin system were analysed using quantitative real-time polymerase chain reaction. A total of 4 849 proteins were identified from LA-1 and LH-1, 697 of which showed differential accumulations. Most of the DEPs have catalytic, binding, and transporter activity and are involved in metabolism- and protein processing-related pathways. In particular, seven DEPs, including two gibberellin (GA) receptors, three cytokinin (CK) receptors, CKoxidase, and CK-N-glucosyltransferase were up-regulated in LA-1, and GA20-oxidase was down-regulated in LH-1. Our results suggest that the DELLA-independent GAsignalling pathway is the primary cause of dwarfism in LA-1 and indicate that CKresponse element 1-2, GA-insensitive dwarf, CKoxidase, and GA3-β-dioxygenase are potential indicators of dwarf cotton. The profiling of DEPs may offer a valuable resource for cotton breeding.

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Abbreviations

ABA:

abscisic acid

ACN:

acetonitrile

CK:

cytokinin

CKX:

cytokinin oxidase

DEPs:

differentially-expressed proteins

GA:

gibberellin

iTRAQ:

isobaric tags for relative and absolute quantitation

MS/MS:

tandem mass spectrometry

NIL:

near-isogenic line

RT-qPCR:

reverse transcription quantitative PCR

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Authors and Affiliations

  1. College of Orient Science and Technology, Hunan Agricultural University, Changsha, 410128, P.R. China

    X. Tu

  2. College of Agronomy, Hunan Agricultural University, Changsha, 410128, P.R. China

    X. Tu, J. Li & A. Liu

  3. College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, P.R. China

    Q. Wang

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  1. X. Tu
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  2. J. Li
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  3. Q. Wang
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  4. A. Liu
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Correspondence to Q. Wang or A. Liu.

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Acknowledgements: This work was supported by grants from the National Natural Science Foundation of China (31571593 and 31301081), the specialized research fund for the doctoral program of higher education (20124320110001), and the youth scientific fund of the Hunan Agricultural University (14QN02). The first two authors contributed equally to this work.

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Tu, X., Li, J., Wang, Q. et al. Quantitative proteomic analysis of upland cotton stem terminal buds reveals phytohormone-related pathways associated with dwarfism. Biol Plant 61, 106–114 (2017). https://doi.org/10.1007/s10535-016-0644-0

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  • DOI: https://doi.org/10.1007/s10535-016-0644-0

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