Abstract
Small heat shock proteins (sHSPs) are crucial components of the plant response to heat shock. We identified and analyzed eight sHSP genes of Capsella bursa-pastoris to better understand the ability of this species to adapt. Eight genes were initially cloned and sequenced from the mature embryo cDNA pool. They belong to the cytosolic I (CI), cytosolic II (CII), and cytosolic III (CIII) subfamilies. One CI sHSP gene was homologous to that of C. rubella. Sequence analysis using 3′ RACE revealed that there are two or more variable 3′-untranslated regions (UTRs) in these sHSP transcripts. The transcriptional levels of the eight sHSP genes were analyzed in different organs and developmental stages via qRT-PCR. Eight genes were significantly up-regulated in young leaves exposed to heat stress at 42 °C, and also showed differential responses to ABA treatment. We also compared expression of these genes with corresponding Arabidopsis sHSP genes and found some differences between the two species.
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Abbreviations
- sHSP:
-
small heat shock protein
- qRT-PCR:
-
quantitatuve reverse transcriptase — polymerase chain reaction
- RACE:
-
rapid amplification of cDNA ends
- 3′ UTR:
-
3′ untranslated region
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Acknowledgments: This work was supported by the State Key Basic Research and Development Plan of China (2007CB108700), the National Natural Science Foundation of China (31070204, 30570112) and the Chinese 111 Project.
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Tao, P., Liu, L. & Wang, J.B. Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris . Biol Plant 56, 648–656 (2012). https://doi.org/10.1007/s10535-012-0239-3
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DOI: https://doi.org/10.1007/s10535-012-0239-3