Abstract
DNA content was estimated by flow cytometry in seedlings and in vitro clones of six species: Oenothera paradoxa, Inula verbascifolia ssp. aschersoniana, Rubus chamaemorus, Solidago virgaurea, S. graminifolia and Pueraria lobata. With the exception of P. lobata, there was no difference in genome sizes between seedlings and in vitro cultured plants from any species, indicative that they maintain their genetic stability during in vitro culture. This confirms the usefulness of tissue culture for production of certified plant material to obtain herbal medicines.
This is a preview of subscription content, log in via an institution to check access.
References
Bajaj, Y.P.S., Furmanowa, M., Olszowska, O.: Biotechnology of the micropropagation of medicinal and aromatic plants.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry, Medicinal and Aromatic Plants. Vol. 4. Pp. 60–103. Springer-Verlag, Berlin-Heidelberg 1988.
Bennett, M.D., Bhandol, P., Leitch, I.J.: Nuclear DNA amounts in angiosperms and their modern uses-807 new estimates.-Ann. Bot. 86: 859–909, 2000.
Canter, P.H., Thomas, H., Ernst, E.: Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology.-Trends Biotechnol. 23: 180–185, 2005.
Kubaláková, M., Doležel, J., Lebeda, A.: Ploidy instability of embryogenic cucumber (Cucumis sativus L.) callus culture.-Biol. Plant. 38: 475–480, 1996.
Loureiro, J., Capelo, A., Brito, G., Rodriguez, E., Silva, S., Pinto, G., Santos, C.: Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry.-Biol. Plant. 51: 7–14, 2007.
Lysák, M.A., Doležel, J.: Estimation of nuclear DNA content in Sesleria (Poaceae).-Caryologia 52: 123–132, 1998.
Price, H.J., Hodnett, G., Johnston, J.S.: Sunflower (Helianthus annuus) leaves contain compounds that reduce nuclear propidium iodide fluorescence.-Ann. Bot. 86: 929–934, 2000.
Skrzypczak, L., Wesołowska, M., Thiem, B., Budzianowski, J.: Solidago L. species (Goldenrod): in vitro regeneration and biologically active secondary metabolites.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Medicinal and Aromatic Plants. Vol. 43. Pp. 384–403. Springer-Verlag, Berlin-Heidelberg 1999.
Sliwinska, E., Zielinska, E., Jedrzejczyk, I.: Are seeds suitable for flow cytometric estimation of plant genome size?-Cytometry 64A: 72–79, 2005.
Sliwinska, E., Zimny, J., Drozdowska, L.: [Instability of ploidy level during regeneration of transformed and nontransformed tobacco (Nicotiana tabacum L.)].-Biotechnologia 3: 260–266, 2003. [In Polish.]
Thiem, B.: Micropropagation of cloudberry (Rubus chamaemorus L.) by initiation of axillary shoots.-Acta Soc. Bot. Pol. 70: 11–16, 2001.
Thiem, B.: In vitro propagation of isoflavone-producing Pueraria lobata (Willd) Ohwi.-Plant Sci. 165: 1123–1128, 2003.
Thiem, B., Skrzypczak, L., Lamer-Zarawska, E.: Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites.-Acta Soc. Bot. Pol. 68: 15–20, 1999.
Thiem, B., Sliwinska, E.: Flow cytometric analysis of nuclear DNA content in cloudberry (Rubus chamaemorus L.) in vitro cultures.-Plant Sci. 164: 129–134, 2003.
Thiem, B., Wesołowska, M., Cis, M.: In vitro culture of Inula verbascifolia ssp. aschersoniana and production of partenolide.-Herba Polon. 49: 37–43, 2003.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sliwinska, E., Thiem, B. Genome size stability in six medicinal plant species propagated in vitro . Biol Plant 51, 556–558 (2007). https://doi.org/10.1007/s10535-007-0121-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-007-0121-x