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Characterization of a planctomycete associated with the marine dinoflagellate Prorocentrum micans Her

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Abstract

During attempts to obtain axenic the cultures of the marine dinoflagellate Prorocentrum micans, a microorganism with peculiar features was isolated. This contaminant resisted the physical and antibiotic treatments performed. Subsequent characterization showed that in agar plates this microorganism develops round granular pink colonies. It is a salt-dependent mesophilic and chemoheterotrophic Gram negative bacterium with a rod to ovoid shape, presenting cell motility in young cultures. Cell division occurs by cell budding. The bacterium forms aggregates with a variable number of cells that are stacked by fibrillar glycoproteic material, the holdfast. A tuft of numerous short glycoproteic fimbriae emerges from one pole of the cell. Preeminent granular inclusions, also of glycoproteic nature, are present in the cytoplasm. Several structural and compositional aspects of the cell envelope and cytoplasm are provided. The production of fibrillar material and the existence of the polar appendages suggest that this microorganism should occur in aquatic environments bound to substrates and could be associated with P. micans in natural marine habitats. Based on the characteristics displayed, this microorganism is a member of the Planctomycetes, order Planctomycetales.

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Acknowledgments

I am grateful to Mrs M. Andrea Costa for excellent technical assistance and to Damien Devos for his encouragement and helpful comments. My special thoughts go to Ana Maria Parente, my supervisor during my PhD that shared with me the discovery of this fascinating group of bacteria.

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Authors and Affiliations

  1. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/nº, 4169-007, Porto, Portugal

    Olga Maria Lage

  2. CIMAR/CIIMAR—Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal

    Olga Maria Lage

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Correspondence to Olga Maria Lage.

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10482_2013_9991_MOESM1_ESM.tif

Online resource 1 Cell section showing details of the cell envelope; (CW) cell wall, (CM) cytoplasmic membrane, (P) periplasm, (F) fimbriae

10482_2013_9991_MOESM2_ESM.tif

Online resource 2 Electron micrographs of thin sections of IP1 labeled after Thiéry procedure. (H) holdfast, (F) fimbriae, (Gl) glycogen-like reserve bodies

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Lage, O.M. Characterization of a planctomycete associated with the marine dinoflagellate Prorocentrum micans Her. Antonie van Leeuwenhoek 104, 499–508 (2013). https://doi.org/10.1007/s10482-013-9991-4

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