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Evaluation of corneal damage caused by iodine preparations using human corneal epithelial cells

  • Laboratory Investigation
  • Published:
Japanese Journal of Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To compare the cytotoxicity of povidone–iodine solution (PVP-I) with that of polyvinyl alcohol–iodine solution (PAI) for ophthalmic use.

Methods

Cells of the human corneal epithelial cell line HCE-T were exposed to various dilutions of PVP-I or PAI, and the cytotoxicity of these two antiseptics was evaluated. The relative toxicities of PVP-I and PAI were also investigated following the inactivation of iodine by achromatization with sodium thiosulfate.

Results

PVP-I and PAI had equivalent antiseptic effects, but the cytotoxicity of PVP-I diluted 16-fold was higher than that of PAI diluted 6-fold. Following inactivation of iodine with sodium thiosulfate, the cytotoxicity of PVP-I remained concentration dependent, whereas PAI exhibited a low toxicity that was similar to the effect of saline on cell viability. Exposure to lauromacrogol, a surfactant used in PVP-I, in solution at concentrations of 1–1000 mg/mL clearly resulted in corneal cytotoxicity. The PVP-I and PAI solutions had a pH value of 4.0 and 5.2, respectively. HCE-T cells were significantly more viable at pH 7 than at pH 1–6.

Conclusion

To avoid corneal damage, preoperative antisepsis of the surgical field should be accomplished with PAI diluted 6-fold, rather than with PVP-I diluted 16-fold. The toxicity of the iodine compound stems primarily from the available iodine concentration and partly from its pH, surfactant and osmolality. Further clinical investigations are required in order to determine the optimal concentrations for use.

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References

  1. Bratzler DW, Dellinger EP, Olsen KM, Perl TM, Auwaerter PG, Bolon MK, et al. Clinical practice guidelines for antimicrobial prophylaxis in surgery. Am J Health-Syst Pharm. 2013;70:233–7.

    Article  Google Scholar 

  2. Ciulla TA, Starr MB, Masket S. Bacterial endophthalmitis prophylaxis for cataract surgery: an evidence based update. Ophthalmology. 2002;109:13–24.

    Article  PubMed  Google Scholar 

  3. Inoue Y, Usui M, Ohashi Y, Shiota H, Yamazaki T. For the preoperative disinfection study group. Preoperative disinfection of the conjunctival sac with antibiotics and iodine compounds: a prospective randomized multicenter study. Jpn J Ophthalmol. 2008;52:151–61.

    Article  CAS  PubMed  Google Scholar 

  4. Inagaki K, Yamaguchi T, Ohde S, Deshpande GA, Kakinoki K, Ohkoshi K. Bacterial culture after three sterilization methods for cataract surgery. Jpn J Ophthalmol. 2013;57:74–9.

    Article  PubMed  Google Scholar 

  5. Ikuno Y, Sawa M, Tsujikawa M, Gomi F, Maeda N, Nishida K. Effectiveness of 1.25% povidone-iodine combined with topical levofloxacin against conjunctival flora in intravitreal injection. Jpn J Ophthalmol. 2012;56:497–501.

    Article  CAS  PubMed  Google Scholar 

  6. Nagai N, Murao T, Oe K, Ito Y, Okamoto N, Shimomura S. In vitro evaluation for corneal damages by anti-glaucoma combination eye drops using human corneal epithelial cell (HCE-T). Yakugaku Zasshi. 2011;131:985–91.

    Article  CAS  PubMed  Google Scholar 

  7. Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, et al. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Visual Sci. 1995;36:614–21.

    CAS  Google Scholar 

  8. Toropainen E, Ranta VP, Talvitie A, Suhonen P, Urtti A. Culture model of human corneal epithelium for prediction of ocular drug absorption. Invest Ophthalmol Visual Sci. 2001;42:2942–8.

    CAS  Google Scholar 

  9. Taliana L, Evans MD, Dimitrijevich SD, Steele JG. The influence of stromal contraction in a wound model system on corneal epithelial stratification. Invest Ophthalmol Visual Sci. 2001;42:81–9.

    CAS  Google Scholar 

  10. Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y, Ueno K. A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta. 1997;44:1299–305.

    Article  CAS  PubMed  Google Scholar 

  11. Isenberg SJ, Apt L, Campeas D. Ocular applications of povidone-iodine. Dermatology. 2002;204:92–5.

    Article  CAS  PubMed  Google Scholar 

  12. Iwasawa A, Nakamura Y. Cytotoxic effect and influence of povidone-iodine on wounds in guinea pig. Kansenshogaku zasshi. 2003;77:948–56.

    PubMed  Google Scholar 

  13. Iwasawa A, Nakamura Y. Antimicrobacterial effects and cytotoxicity of povidone-iodine preparation: influence of additional surfactant. Jpn J Environ infect. 2001;16:179–83.

    Google Scholar 

  14. Gonnering R, Edelhauser HF, Van Horn DL, Durant W. The pH tolerance of rabbit and human corneal endothelium. Invest Ophthalmol Visual Sci. 1979;18:373–90.

    CAS  Google Scholar 

  15. Edelhauser HF, Hanneken AM, Pederson HJ, Van Horn DL. Osmotic tolerance of rabbit and human corneal endothelium. Arch Ophthalmol. 1981;99:1281–7.

    Article  CAS  PubMed  Google Scholar 

  16. Wille H. Assessment of possible toxic effects of polyvinylpyrrolidone-iodine upon the human eye in conjunction with cataract extraction. An endothelial specular microscope study. Acta Ophthalmol. 1982;60:955–60.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Professional editing of the English language in this article was performed by Editage (Cactus Communications).

Conflicts of interest

Y. Shibata, None; Y. Tanaka, None; T. Tomita, None; T. Taogoshi, None; Y. Kimura, None; T. Chikama, None; K. Kihira, None.

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Authors and Affiliations

  1. Department of Pharmaceutical Services, Hiroshima University Hospital, 1-2-3 Kasumi, Minami-ku, Hiroshima, Hiroshima, 734-8551, Japan

    Yuuka Shibata, Takashi Tomita, Takanori Taogoshi, Yasuhiro Kimura & Kenji Kihira

  2. Faculty of Pharmaceutical Sciences, Hiroshima University, Hiroshima, Japan

    Yuta Tanaka

  3. Department of Ophthalmology and Visual Science, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

    Taiichiro Chikama

Authors
  1. Yuuka Shibata
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  2. Yuta Tanaka
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  3. Takashi Tomita
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  4. Takanori Taogoshi
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  5. Yasuhiro Kimura
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  6. Taiichiro Chikama
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  7. Kenji Kihira
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Corresponding author

Correspondence to Yuuka Shibata.

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Shibata, Y., Tanaka, Y., Tomita, T. et al. Evaluation of corneal damage caused by iodine preparations using human corneal epithelial cells. Jpn J Ophthalmol 58, 522–527 (2014). https://doi.org/10.1007/s10384-014-0348-y

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  • DOI: https://doi.org/10.1007/s10384-014-0348-y

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