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Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina

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Abstract

By taking advantage of large cell size of Chara corallina, we analyzed the membrane depolarization induced by decreased turgor pressure (Shimmen in J Plant Res 124:639–644, 2011). In the present study, the response to increased turgor pressure was analyzed. When internodes were incubated in media containing 200 mM dimethyl sulfoxide, their intracellular osmolality gradually increased and reached a steady level after about 3 h. Upon removal of dimethyl sulfoxide, turgor pressure quickly increased. In response to the increase in turgor pressure, the internodes generated a transient membrane depolarization at its nodal end. The refractory period was very long and it took about 2 h for full recovery after the depolarizing response. Involvement of protein synthesis in recovery from refractoriness was suggested, based on experiments using inhibitors.

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Abbreviations

APW:

Artificial pond water

APW(200 DMSO):

APW supplemented with 200 mM DMSO

CHI:

Cycloheximide

DMSO:

Dimethyl sulfoxide

EAB :

Potential difference between pools A and B

EG:

Ethylene glycol

Пi :

Intracellular osmolality

Πo :

Extracellular osmolality

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Acknowledgments

The authors thank Prof. Nobuaki Yanagihara (School of Medicine, University of Occupational and Environmental Health) for his kind supports of the present experiments.

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Authors and Affiliations

  1. Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297, Japan

    Teruo Shimmen

  2. School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishiku, Fukuoka, 807-8555, Japan

    Koreaki Ogata

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  1. Teruo Shimmen
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  2. Koreaki Ogata
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Correspondence to Teruo Shimmen.

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Shimmen, T., Ogata, K. Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina . J Plant Res 126, 439–446 (2013). https://doi.org/10.1007/s10265-012-0537-z

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  • DOI: https://doi.org/10.1007/s10265-012-0537-z

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