Skip to main content

Advertisement

SpringerLink
Log in

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

  • Original Article
  • Published:
Functional & Integrative Genomics Aims and scope Submit manuscript

Abstract

Controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. This is significant for eliciting disease-resistant features in crops through genetic engineering. The germins and germin-like proteins (GLPs) are known to be associated with plant and developmental stages. The 1107-bp Oryza sativa root GLP2 (OsRGLP2) gene promoter fused to a β-glucuronidase (GUS) reporter gene was transformed into potato plants through an Agrobacterium-mediated transformation. The OsRGLP2 promoter was activated in response to Fusarium solani (Mart.) Sacc. and Alternaria solani Sorauer. Quantitative real-time PCR results revealed 4–5-fold increase in promoter activity every 24 h following infection. There was a 15-fold increase in OsRGLP2 promoter activity after 72 h of F. solani (Mart.) Sacc. treatment and a 12-fold increase observed with A. solani Sorauer. Our results confirmed that the OsRGLP2 promoter activity was enhanced under fungal stress. Furthermore, a hyperaccumulation of H2O2 in transgenic plants is a clear signal for the involvement of OsRGLP2 promoter region in the activation of specific genes in the potato genome involved in H2O2-mediated defense response. The OsRGLP2 promoter evidently harbors copies of GT-I and Dof transcription factors (AAAG) that act in response to elicitors generated in the wake of pathogen infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Angelini R, Tisi A, Rea G, Chen MM, Botta M, Federico R, Cona A (2008) Involvement of polyamine oxidase in wound healing. Plant Physiol 146:162–177

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Banerjee AK, Prat S, Hannapel DJ (2006) Efficient production of transgenic potato (S. tuberosum L. andigena) plants via Agrobacterium tumefaciens mediated transformation. Plant Sci 170:732–738

    Article  CAS  Google Scholar 

  • Breen J, Bellgard M (2010) Germin-like proteins (GLPs) in cereal genomes: gene clustering and dynamic roles in plant defense. Funct Integr Genomics 10:463–476

    Article  CAS  PubMed  Google Scholar 

  • Chakravarty B, Wang-Pruski G (2010) Rapid regeneration of stable transformants in cultures of potato by improving factors influencing Agrobacterium mediated transformation. Adv Biosci Biotechnol 1:409–416

    Article  CAS  Google Scholar 

  • Chaouch S, Queval G, Vanderauwera S, Mhamdi A, Vandorpe M, LangloisMeurinne M, Van Breusegem F, Saindrenan P, Noctor G (2010) Genetic reversion of cell death in the Arabidopsis cat2 knockout mutant shows that peroxisomal H2O2 is coupled to biotic responses by isochorismate synthase 1 in a day length-related manner. Plant Physiol 153:1692–1705

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Cingel A, Vinterhalter B, Vinterhalter D, Ali-Dragosavac D, Smigocki A, Ninkovic S (2010) Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragaevka and cv. Jelica). Afr J Biotechnol 9:4644–4650

    CAS  Google Scholar 

  • Clarke HR, Davis JM, Wilbert SM, Bradshaw HD Jr, Gordon MP (1994) Wound induced and developmental activation of a poplar tree chitinase gene promoter in transgenic tobacco. Plant Mol Biol 25:799–815

    Article  CAS  PubMed  Google Scholar 

  • Delporte A, Sofie VH, Els JM, Damme V (2013) Qualitative and quantitative analysis of the Nictaba promoter activity during development in Nicotiana tabacum. Plant Physiol Biochem 67:162–168

    Article  CAS  PubMed  Google Scholar 

  • Dong X, Ji R, Guo X, Foster SJ, Chen H, Dong C, Liu Y, Hu Q, Liu S (2008) Expressing a gene encoding wheat oxalate oxidase enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus). Planta 228:331–340

    Article  CAS  PubMed  Google Scholar 

  • Hernandez-Garcia CM, Finer JJ (2013) Identification and validation of promoters and cis acting regulatory elements. Plant Sci 217–218:109–119

    PubMed  Google Scholar 

  • Huffaker A, Pearce G, Ryan C (2006) An endogenous peptide signal in Arabidopsis activates components of the innate immune response. Proc Natl Acad Sci U S A 103:10098–10103

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hung SH, Yu CW, Lin CH (2005) Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin 46:1–10

    CAS  Google Scholar 

  • Huttly A (2009) Reporter genes. Methods Mol Biol 478:39–69

    Article  PubMed  Google Scholar 

  • Kumar M, Chimote V, Singh R, Mishra GP, Nail PS, Pandey SK, Chakrabarti SK (2010) Development of Bt transgenic potatoes for effective control of potato tuber moth by using cry1Ab gene regulated by GBSS promoter. Crop Prot 29:121–127

    Article  CAS  Google Scholar 

  • Lee SC, Kim DS, Kim NH, Hwang BK (2007) Functional analysis of the promoter of the pepper pathogen-induced gene, CAPIP2, during bacterial infection and abiotic stresses. Plant Sci 172:236–245

    Article  CAS  Google Scholar 

  • Maghuly F, Khan MA, Fernandez EB, Druart P, Watillon B, Laimer M (2008) Stress regulated expression of the GUS-marker gene (uidA) under the control of plant calmodulin and viral 35S promoters in a model fruit tree rootstock: Prunus incisa × serrula. J Biotechnol 135:105–116

    Article  CAS  PubMed  Google Scholar 

  • Mahmood T, Hyder MZ, Naqvi SMS (2007) Cloning and sequence analysis of germin-like protein gene 2 promoter from Oryza sativa L. ssp indica. DNA Seq 18:26–32

    Article  CAS  PubMed  Google Scholar 

  • Mahmood T, Yasmin T, Haque MI, Naqvi SMS (2013) Characterization of a rice germin-like protein gene promoter. Genet Mol Res 12:360–369

    Article  CAS  Google Scholar 

  • Manosalva PM, Davidson RM, Liu B, Zhu X, Hulbert SH, Leung H, Leach JE (2009) A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice. Plant Physiol 149:286–296

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Matzke MA, Matzke AJM (1995) How and why do plants inactivate homologous (trans) genes? Plant Physiol 107:679–685

    PubMed Central  CAS  PubMed  Google Scholar 

  • Meiyalaghan S, Jacobs JME, Butler RC, Wratten SD, Conner AJ (2006) Expression of cry1Ac9 and cry9Aa2 genes under a potato light-inducible Lhca3 promoter in transgenic potatoes for tuber moth resistance. Euphytica 147:297–309

    Article  CAS  Google Scholar 

  • Munir F, Naqvi SMS, Mahmood T (2011) In vitro culturing and assessment of somaclonal variation of Solanum tuberosum var. desiree. Turk J Biochem 36:296–302

    CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Osusky M, Zhou G, Osuska L, Hancock RE, Kay WW, Misra S (2000) Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat Biotechnol 18:1162–1166

    Article  CAS  PubMed  Google Scholar 

  • Richards EJ (1997) Preparation of plant DNA using CTAB. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Short protocols in molecular biology, 3rd edn. John Wiley, New York, pp 10–11, 2

    Google Scholar 

  • Rosellini D (2011) Selectable marker genes from plants: reliability and potential. In Vitro Cell Dev Biol Plant 47:222–223

    Article  CAS  Google Scholar 

  • Singh HR, Deka M, Das S (2015) Enhanced resistance to blister blight in transgenic tea (Camellia sinensis [L.] O. Kuntze) by overexpression of class I chitinase gene from potato (Solanum tuberosum). Funct Integr Genomics 15:461–480

    Article  CAS  PubMed  Google Scholar 

  • Tai HH, Goyer C, Platt HW, De Koeyer D, Murphy A, Uribe P, Halterman D (2013) Decreased gene expression in tolerance versus resistance to Verticillium dahliae in potato. Funct Integr Genomics 13:367–378

    Article  CAS  PubMed  Google Scholar 

  • Tanabe N, Tamoi M, Shigeoka S (2015) The sweet potato RbcS gene (IbRbcS1) promoter confers high level and green tissue-specific expression of the GUS reporter gene in transgenic Arabidopsis. Gene 567:244–250

    Article  CAS  PubMed  Google Scholar 

  • Wu AM, Ling C, Liu JY (2006) Isolation of a cotton reversibly glycosylated polypeptide (GhRGP1) promoter and its expression activity in transgenic tobacco. J Plant Physiol 163:426–435

    Article  CAS  PubMed  Google Scholar 

  • Yang L, Li T, Zhang SC, Gao GL, Yang CW (2013) Characterization of the GLP13 gene promoter in Arabidopsis thaliana. Biol Plant 57:231–237

    Article  CAS  Google Scholar 

  • Yevtushenko DP, Sidorov VA, Romero R, Kay WW, Misra S (2004) Wound-inducible promoter from poplar is responsive to fungal infection in transgenic potato. Plant Sci 167:715–724

    Article  CAS  Google Scholar 

  • Yogendra KN, Pushpa D, Mosa KA, Kushalappa AC, Murphy A, Mosquera T (2014) Quantitative resistance in potato leaves to late blight associated with induced hydroxycinnamic acid amides. Funct Integr Genomics 14:285–298

    Article  CAS  PubMed  Google Scholar 

  • Zhang H, Jin J, Tang L, Zhao Y, Gu X, Gao G, Luo J (2011) Plant TFDB 2.0: update and improvement of the comprehensive plant transcription factor database. Nucleic Acids Res 48:1114–1117

    Article  Google Scholar 

Download references

Acknowledgments

We are thankful to the Higher Education Commission, Islamabad, Pakistan for providing financial assistance. We are grateful to the Centre for Integrative Legume Research—CILR lab, the University of Queensland, Australia for the qRT-PCR facility and to the Queensland Plant Pathology Herbarium, Australia for providing the potato-specific fungal strains.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

    Faiza Munir

  2. Center for Integrative Legume Research, School of Agriculture and Food Sciences, University of Queensland, Brisbane, Australia

    Satomi Hayashi

  3. School of Plant Biology, University of Western Australia, Perth, Australia

    Jacqueline Batley

  4. Department of Biochemistry, University of Arid Agriculture Rawalpindi, Punjab, Pakistan

    Syed Muhammad Saqlan Naqvi

  5. Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

    Tariq Mahmood

Authors
  1. Faiza Munir
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satomi Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacqueline Batley
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Syed Muhammad Saqlan Naqvi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tariq Mahmood
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tariq Mahmood.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Munir, F., Hayashi, S., Batley, J. et al. Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants. Funct Integr Genomics 16, 19–27 (2016). https://doi.org/10.1007/s10142-015-0463-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10142-015-0463-y

Keywords

Search

Navigation