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The effect of He-Ne and Ga-Al-As laser light on the healing of hard palate mucosa of mice

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Abstract

Low-level laser therapy (LLLT) has been used to accelerate wound healing, yet questions remain concerning its therapeutic applications. This study aimed to compare the healing efficacy of helium-neon (He-Ne) red light (laser) and gallium aluminum arsenide (Ga-Al-As) infrared lasers at two different doses on hard palate wounds. In a randomized controlled study, 75 adult male mice were divided into five groups of 15 each, after undergoing identical surgical procedures; a control group, with no laser irradiation; HD1 and HD2 groups, treated with He-Ne laser (wavelengths 632.8 nm, power 5 mW, and spot size 0.02 cm2) at doses of 4 J/cm2 and 7.5 J/cm2 respectively; and GD1 and GD2 groups, treated with Ga-Al-As laser (wavelengths 830 nm, peak power 25 mW, and spot size 0.10 cm2) at the doses of 4 J/cm2 and 7.5 J/cm2, respectively. Five animals from each group were killed on the third, seventh, and 14 days after surgery, and biopsies were made for histological analysis. On the 3rd and 7th day after the surgery, the number of polymorphonuclear cells (PMN) in HD1, HD2, GD1, and GD2 groups was significantly lower than that of the control group. On the 7th and 14th day, the fibroblasts and new blood vessels counts and collagen density fibers in HD1, HD2, GD1, and GD2 groups were also significantly higher than that of the control groups, and the fibroblast counts and collagen density fibers in HD1 and HD2 groups were higher than that of the GD1 and GD2 groups. LLLT with He-Ne laser compared to Ga-Al-As laser has a positive healing effect on hard palate gingival wounds in mice regardless of the radiation dose.

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Authors and Affiliations

  1. Iran Center for Dental Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Farahnaz Fahimipour

  2. Department of Oral and Maxillofacial Radiology, University of Connecticut School of Dental Medicine, Farmington, CT, USA

    Mina Mahdian

  3. Department of Periodontics, Shahid Beheshti University of Medical Science, Tehran, Iran

    Behzad Houshmand

  4. Department of Endodontic, Shahid Beheshti University of Medical Science, Tehran, Iran

    Mohammad Asnaashari

  5. Department of Industrial Management, Yazd University, Yazd, Iran

    Alireza Naser Sadrabadi

  6. Amirkabir University of Technology, Tehran, Iran

    Seid Emadadin Najafi Farashah

  7. Shahed University, Tehran, Iran

    Seid Mohsen Mousavifard

  8. Department of Oral and Maxillofacial Surgery, Iran Center for Dental Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Arash Khojasteh

  9. 1475 Folsom St, Apt 1026, Boulder, CO, 80302, USA

    Mina Mahdian

Authors
  1. Farahnaz Fahimipour
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  2. Mina Mahdian
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  3. Behzad Houshmand
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  4. Mohammad Asnaashari
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  5. Alireza Naser Sadrabadi
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  6. Seid Emadadin Najafi Farashah
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  7. Seid Mohsen Mousavifard
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  8. Arash Khojasteh
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Correspondence to Mina Mahdian.

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Fahimipour, F., Mahdian, M., Houshmand, B. et al. The effect of He-Ne and Ga-Al-As laser light on the healing of hard palate mucosa of mice. Lasers Med Sci 28, 93–100 (2013). https://doi.org/10.1007/s10103-012-1060-0

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  • DOI: https://doi.org/10.1007/s10103-012-1060-0

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