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Raman spectroscopy of parathyroid tissue pathology

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Abstract

Primary hyperparathyroidism (HPT) in 80% of patients is due to a solitary parathyroid adenoma, while in 20% multigland pathology exists, usually hyperplasia [Scott-Coombes, Surgery, 21(12):309–312, 2003]. Despite recent advances in minimally invasive parathyroidectomy, better preoperative localisation techniques and intraoperative parathyroid hormone (PTH) monitoring, a 4% failure rate [Grant CS, Thompson G, Farley D, Arch Surg, 140:47–479, 2005] persists making accurate differentiation between adenomas and hyperplasia of prime importance. We investigated the ability of Raman spectroscopy to accurately differentiate between parathyroid adenomas and hyperplasia. Raman spectra were measured at defined points on the parathyroid tissue sections using a bench-top microscopy system. Multivariate analysis of the spectra was carried out to construct a diagnostic algorithm correlating spectral results with the histopathological diagnosis. A total of 698 spectra were analysed. Principal-component (PCA)-fed linear discriminant analysis (LDA) used to construct a diagnostic algorithm. Detection sensitivity for parathyroid adenomas was 95% and hyperplasia was 93%. These preliminary results indicate that Raman spectroscopy is potentially an excellent tool to differentiate between parathyroid adenomas and hyperplasia.

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Acknowledgements

Dept. of Pathology Gloucestershire Royal Hospital for their help and support.

Dr Sare Paul Professor of Pathology (Retd) Madras Medical College, India.

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Authors and Affiliations

  1. Department of Breast and Endocrine Surgery, Gloucestershire Royal Hospital, Great Western Road, Gloucester, GL1 3NN, UK

    Kaustuv Das & Clare Fowler

  2. Biophotonics Research Group, Gloucestershire Royal Hospital, Gloucester, UK

    Nicholas Stone & Catherine Kendall

  3. Dept. of Pathology, Gloucestershire Royal Hospital, Gloucester, GL1 3NN, UK

    J. Christie-Brown

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  1. Kaustuv Das
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  2. Nicholas Stone
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  3. Catherine Kendall
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  4. Clare Fowler
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  5. J. Christie-Brown
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Correspondence to Nicholas Stone.

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Das, K., Stone, N., Kendall, C. et al. Raman spectroscopy of parathyroid tissue pathology. Lasers Med Sci 21, 192–197 (2006). https://doi.org/10.1007/s10103-006-0397-7

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  • DOI: https://doi.org/10.1007/s10103-006-0397-7

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