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French regional surveillance program of carbapenemase-producing Gram-negative bacilli: results from a 2-year period

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Abstract

In February 2011, the CARB-LR group was created as a sentinel laboratory-based surveillance network to control the emergence of carbapenem-resistant Gram-negative bacilli (CR GNB) in a French Southern Region. We report the epidemiological results of a 2-year study. All the Gram-negative bacilli isolates detected in the different labs (hospital and community settings) of a French Southern Region and with reduced susceptibility to ertapenem and/or imipenem were characterised with regard to antibiotic resistance, bla genes content, repetitive sequence-based polymerase chain reaction (rep-PCR) profiles and multilocus sequence typing (MLST). A total of 221 strains were analysed. Acinetobacter baumannii was the most prevalent carbapenemase-producing bacteria, with a majority of OXA-23 producers (n = 37). One isolate co-produced OXA-23 and OXA-58 enzymes. Klebsiella pneumoniae was the most frequent carbapenemase-producing Enterobacteriaceae (CPE) (OXA-48 producer: n = 29, KPC producer: n = 1), followed by Escherichia coli (OXA-48 producer: n = 8, KPC producer: n = 1) and Enterobacter cloacae (OXA-48 producer, n = 1). One isolate of Pseudomonas aeruginosa produced a VIM-1 carbapenemase. A clonal diversity of carbapenemase-producing K. pneumoniae and E. coli was noted with different MLSTs. On the other hand, almost all OXA-23-producing A. baumannii strains belonged to the widespread ST2/international clone II. The link between the detection of CR GNB and a foreign country was less obvious, suggesting the beginning of a local cross-transmission. The number of CR GNB cases in our French Southern Region has sharply increased very recently due to the diffusion of OXA-48 producers.

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Acknowledgements

INSERM U1047 is supported by INSERM and Université de Montpellier 1.

Conflict of interest

The authors report no conflicting interests.

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Authors and Affiliations

  1. Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes Cedex 09, 30029, France

    A. Pantel & J.-P. Lavigne

  2. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, Nîmes, France

    A. Pantel, A. Boutet-Dubois, A. Sotto & J.-P. Lavigne

  3. Laboratoire de Biologie Polyvalente, CH Alès-Cévennes, Alès, France

    A. Boutet-Dubois

  4. Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier, France

    H. Jean-Pierre & H. Marchandin

  5. Service des Maladies Infectieuses et Tropicales, CHU Carémeau, Nîmes, France

    A. Sotto

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Correspondence to J.-P. Lavigne.

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CARB-LR group: Fabrice Amiel (Bioaxiome, Nîmes), Jeremy Bayette (Biomed34, St Thibéry), Brigitte Compan (Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier), Jean-Marie Courrège (Laboratoire Polyvalent, CH Bagnols-sur-Cèze), Marie-Noëlle Didelot-Rousseau (Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier), Anne Gaschet (Laboratoire de Biologie Polyvalente, Hôpital St Jean, Perpignan), Sylvain Godreuil (Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier), Brigitte Lamy (Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier), Anne-Laure Michon (Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier), Pierre Mion (LaboSud OC Biologie, Montpellier), Eugénie Mirouse (LaboSud OC Biologie, Béziers), Isabelle Pagès (LaboSud OC Biologie, Montpellier), Jocelyne Paillisson (LaboSud OC Biologie, Montpellier), Rémy Fournier (LaboSud OC Biologie, Montpellier), Bruno Poirey (Unibio, Nîmes).

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Pantel, A., Boutet-Dubois, A., Jean-Pierre, H. et al. French regional surveillance program of carbapenemase-producing Gram-negative bacilli: results from a 2-year period. Eur J Clin Microbiol Infect Dis 33, 2285–2292 (2014). https://doi.org/10.1007/s10096-014-2189-5

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