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High-frequency 263 GHz PELDOR

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Abstract

Pulsed electron–electron double resonance (PELDOR/DEER) at high frequencies can provide information on the relative orientation of paramagnetic centres or spin labels, if those are rigidly oriented in a host biomolecule and experiments are performed with sufficient orientation selectivity. We present the first comparative PELDOR study at 263 and 94 GHz on a model RNA system containing rigid nitroxides. We show that at 263 GHz still considerable modulation depth is observed and orientation selectivity is significant, particularly in g x–g y plane of the nitroxides.

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Acknowledgments

This work has been supported by the Max Planck Society and the Deutsche Forschungsgemeinschaft (SFB 803). We thank Igor Gromov and Peter Höfer (Bruker BioSpin) for their collaborative work and support in implementing the E780 spectrometer, Snorri Th. Sigurdsson (University of Iceland) for providing the Çm spin label and Brigitta Angerstein (MPIbpc, Göttingen) for the help in sample preparation.

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Authors and Affiliations

  1. Research Group EPR Spectroscopy, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany

    Igor Tkach, Karin Halbmair & Marina Bennati

  2. Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany

    Claudia Höbartner

  3. Institute of Organic and Biomolecular Chemistry, Georg-August University of Göttingen, Tammannstr. 2, Göttingen, Germany

    Claudia Höbartner & Marina Bennati

Authors
  1. Igor Tkach
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  2. Karin Halbmair
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  3. Claudia Höbartner
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  4. Marina Bennati
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Correspondence to Igor Tkach.

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Tkach, I., Halbmair, K., Höbartner, C. et al. High-frequency 263 GHz PELDOR. Appl Magn Reson 45, 969–979 (2014). https://doi.org/10.1007/s00723-014-0581-z

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  • DOI: https://doi.org/10.1007/s00723-014-0581-z

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