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Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus

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Abstract

Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae. Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted.

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Authors and Affiliations

  1. National Institute of Agrobiological Sciences, Owashi, Tsukuba, Ibaraki, 305-8634, Japan

    Nobuhiko Nakashima & Yuri Nakamura

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  1. Nobuhiko Nakashima
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  2. Yuri Nakamura
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Correspondence to Nobuhiko Nakashima.

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Nakashima, N., Nakamura, Y. Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus. Arch Virol 153, 1955–1960 (2008). https://doi.org/10.1007/s00705-008-0208-5

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  • DOI: https://doi.org/10.1007/s00705-008-0208-5

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