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Effect of nitrate on anaerobic azo dye reduction

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Abstract

The aim of the study was to investigate the effect of nitrate on anaerobic color removal efficiencies. For this aim, anaerobic–aerobic sequencing batch reactor (SBR) fed with a simulated textile effluent including Remazol Brilliant Violet 5R azo dye was operated with a total cycle time of 12 h, including anaerobic (6 h) and aerobic cycles (6 h). Microorganism grown under anaerobic phase of the reactor was exposed to different amounts of competitive electron acceptor (nitrate) and performance of the system was determined by monitoring color removal efficiency, nitrate removal, nitrite formation and removal, oxidation reduction potential, color removal rate, chemical oxygen demand (COD), specific anaerobic enzyme (azo reductase) and aerobic enzyme (catechol 1,2 dioxygenase), and formation and removal of aromatic amines. Variations of population dynamics of microorganisms exposed to various amount of nitrate were identified by denaturing gradient gel electrophoresis (DGGE). It was found that nitrate has adverse effect on anaerobic color removal efficiency and color removal was achieved after denitrification process was completed. It was found that nitrate stimulates the COD removal efficiency and accelerates the COD removal in the first hour of anaerobic phase. About 90 % total COD removal efficiencies were achieved in which microorganism exposed to increasing amount of nitrate. Population dynamics of microorganisms exposed to various amount of nitrate were changed and diversity was increased.

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References

  1. Lourenço ND, Novais JM, Pinheiro HM (2001) Effect of some operational parameters on textile dye biodegradation in a sequential batch reactor. J Biotechnol 89:163–174

    Article  Google Scholar 

  2. Shaw C, Carliell CM, Wheatley AD (2002) Anaerobic/aerobic treatment of coloured textile effluents using sequencing batch reactors. Water Res 36:1993–2001

    Article  CAS  Google Scholar 

  3. Carliell CM, Barclay SJ, Naidoo N (1995) Microbial decolourasition of a reactive azo dye under anaerobic conditions. Water SA 21:61–69

    CAS  Google Scholar 

  4. Gottlieb A, Shaw C, Smith A, Wheatley A, Forsythe S (2003) The toxicity of textile reactive azo dyes after hydrolysis and decolourisation. J Biotechnol 101:49–56

    Article  CAS  Google Scholar 

  5. Haug W, Schmidt A, Nortemann B, Hempel DC, Stolz A, Knackmuss HJ (1991) Mineralization of the sulfonated azo dye mordant yellow 3 by a 6-aminophtalene-2-sulfonate-degrading bacterial consortium. Appl Environ Microbiol 57:3144–3149

    CAS  Google Scholar 

  6. Hu TL (1998) Degradation of azo dye RP2B by Pseudomonas luteola. Water Sci Technol 38:299–306

    CAS  Google Scholar 

  7. Kudlich M, Bishop P, Knackmuss HJ, Stolz A (1996) Simultaneous anaerobic and aerobic degradation of the sulfonated azo dye Mordant Yellow 3 by immobilized cells from a naphthalenesulfonate-degrading mixed culture. Appl Microbiol Biotechnol 46:597–603

    Article  CAS  Google Scholar 

  8. Seshadri S, Bishop PL, Agha AM (1994) Anaerobic/aerobic treatment of selected azo dyes in wastewater. Waste Manage 14:127–137

    Article  CAS  Google Scholar 

  9. Zaoyan Y, Ke S, Guangliang S, Fan Y, Jinshan D, Huanian M (1992) Anaerobic–aerobic treatment of a dye wastewater by combination of RBC with activated sludge. Water Sci Technol 26:2093–2096

    CAS  Google Scholar 

  10. Van der Zee FP, Villaverde S (2005) Combined anaerobic aerobic treatment of azo dyes: a short review of bioreactor studies. Water Res 39:1425–1440

    Article  Google Scholar 

  11. Çınar Ö, Kertmen S, Yaşar M, Demiröz K, Yigit NÖ, Kitis M (2008) Effect of cycle time on biodegradation of azo dye in sequencing batch reactor. Process Safety Environ Prot 86:455–460

    Article  Google Scholar 

  12. Chang JS, Chien C, Lin YC, Lin PJ, Ho JY, Hu TL (2001) Kinetic characteristics of bacterial azo-dye decolorization by Pseudomonas luteola. Water Res 35(12):2841–2850

    Article  CAS  Google Scholar 

  13. Çınar Ö, Demiröz K, Kanat G, Uysal Y, Yaman C (2009) The effect of oxygen on anaerobic color removal of azo dye in sequencing batch reactor. Clean 37(8):657–662

    Google Scholar 

  14. Ramalho PA, Cardoso MH, Cavaco-Paulo A, Ramalho MT, (2004) Characterization of azo reduction activity in a novel ascomycete yeast strain. Appl Environ Microbiol 2279–2288

  15. Carliell CM, Barclay SJ, Shaw C, Wheatley AD, Buckley CA (1998) The effect of salts used in textile dyeing on microbial decolourisation of a reactive azo dye. Environ Technol 19:1133–1137

    Article  CAS  Google Scholar 

  16. Dos Santos AB, Brauna CHC, Mota S, Cervantes FJ (2008) Effect of nitrate on the reduction of Reactive Red 2 by mesophilic anaerobic sludge. Water Sci Techonol 57:1067–1071

    Article  Google Scholar 

  17. Pearce CI, Christie R, Boothman C (2006) Reactive azo dye reduction by Shewanella Strain J18 143. Biotechnol Bioeng 95:692–703

    Article  CAS  Google Scholar 

  18. Lourenço ND, Novais JM, Pinheiro HM (2000) Reactive textile dye colour removal in a sequencing batch reactor. Water Sci Technol 42:321–328

    Google Scholar 

  19. Wuhrmann K, Mechsner K, Kappeler T (1980) Investigation on rate-determining factors in the microbial reduction of azo dyes. Appl Microbiol Biotechnol 9:325–338

    Article  CAS  Google Scholar 

  20. Clesceri LS, Greenberg AE, Eaton AD (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association and Water Environment Federation, Washington DC

    Google Scholar 

  21. Garrigos MC, Reche F, Marin ML (2002) Determination of aromatic amines formed from azo colorants in toy product. J Chromatogr 976:309–317

    Article  CAS  Google Scholar 

  22. Kolomytseva MP, Baskunov BP, Golovleva LA (2007) Intradiol pathway of para-cresol conversion by Rhodococcus Opacus 1CP. J Biotechnol 2:886–893

    Article  CAS  Google Scholar 

  23. Russ R, Rau J, Stolz A (2000) The function of cytoplasmic flavin reductases in the reduction of azo dyes by bacteria. Appl Environ Microbiol 66(4):1429–1434

    Article  CAS  Google Scholar 

  24. Daniels L, Hanson RS, Philips JA (1994) Chemical analysis in methods for general and molecular bacteriology. In: Gerhart P (ed) American Society For Microbiology, Washington, DC, pp 512–554

  25. Su Y, Zhang Y, Wang J, Zhou J, Lu X, Lu H (2009) Enhanced bio-decolorization of azo dyes by co-immobilized quinone-reducing consortium and anthraquinone. Bioresour Technol 100:2982–2987

    Article  CAS  Google Scholar 

  26. Dubin P, Wright KL (1975) Reduction of azo food dyes in cultures of Proteus vulgaris. Xenobiotica 5(9):563–571

    Article  CAS  Google Scholar 

  27. Dos Santos AB, Cervantes FJ, Van Lier JB (2007) Review paper on current technologies for decolourisation of textile wastewaters perspectives for anaerobic biotechnology. Bioresour Technol 98:2369–2385

    Article  Google Scholar 

  28. Ong SA, Toorisaka E, Hirata M, Hano T (2008) Granular activated carbon biofilm configured sequencing batch reactor treatment of C.I. Acid Orange 7. Dyes Pigments 76:142–146

    Article  Google Scholar 

  29. Panswand T, Techovanich A, Anotai J (2001) Comparison of dye wastewater treatment by normal anoxic plus anaerobic/aerobic SBR activated sludge process. Water Sci Technol 43:355–362

    Google Scholar 

  30. Kapdan IK, Özturk R (2005) Effect of parameters on color and cod removal performance of SBR: sludge age and initial dyestuff concentration. J Hazard Mater 123:217–222

    Article  CAS  Google Scholar 

  31. Panswand T, Luangdilok W (2000) Decolorization of reactive dyes with different molecular structures under different environmental conditions. Water Res 34(17):4177–4184

    Article  Google Scholar 

  32. Daizong C, Li G, Zhao D, Gu X, Wang C, Zhao M (2012) Purification and characterization of an azoreductase from Escherichia coli CD-2 possessing quinone reductase activity. Process Biochem 47:544–549

    Article  Google Scholar 

  33. Waghmode TR, Kurade MB, Khandare RV, Govindwar SP (2011) A sequential aerobic/microaerophilic decolorization of sulfonated mono azo dye Golden Yellow HER by microbial consortium GG-BL. Int Biodeterior Biodegrad 65:1024–1034

    Article  CAS  Google Scholar 

  34. Wang CL, You SL, Wang SL (2006) Purification and characterization of a novel catechol 1,2-dioxygenase from Pseudomonas aeruginosa with benzoic acid as a carbon source. Process Biochem 41:1594–1601

    Article  CAS  Google Scholar 

  35. Çınar Ö (2002) Factors influencing biodegradation of benzoate by denitrifying bacterial enrichment cultures. PhD Dissertation Clemson University, Clemson SC

  36. Altenschmidt U, Oswald B, Steiner E et al (1993) New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and a 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp. J Bacteriol 175:4851–4858

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Environmental Engineering, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, 46060-9, Turkey

    Kevser Cirik & Özer Çinar

  2. Department of Environmental Engineering, Süleyman Demirel University, Isparta, 32260, Turkey

    Mehmet Kitiş

  3. Department of Genetics and Bioengineering, International University of Sarajevo, Hrasnicka Cesta 15, 71210, Sarajevo, Bosnia and Herzegovina

    Özer Çinar

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  1. Kevser Cirik
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  2. Mehmet Kitiş
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  3. Özer Çinar
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Correspondence to Özer Çinar.

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Cirik, K., Kitiş, M. & Çinar, Ö. Effect of nitrate on anaerobic azo dye reduction. Bioprocess Biosyst Eng 36, 69–79 (2013). https://doi.org/10.1007/s00449-012-0762-9

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  • DOI: https://doi.org/10.1007/s00449-012-0762-9

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