Skip to main content

Advertisement

SpringerLink
Log in

Operating conditions optimization for (+)-terrein production in a stirred bioreactor by Aspergillus terreus strain PF-26 from marine sponge Phakellia fusca

  • Short Communication
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

Terrein is a fungal metabolite with application values in the fields of medicine, cosmetology, and agriculture. However, mass production of single configuration terrein is still a big challenge. In this study, operating factors such as inoculation, agitation speed, aeration rate, pH control, and nutrient feeding were preliminarily optimized to improve the (+)-terrein production in the 5-L stirred bioreactor from the marine sponge-derived fungus Aspergillus terreus PF-26. Spore inoculation, low agitation speed, and aeration rate were proved to be suitable for A. terreus PF-26 to produce (+)-terrein in the stirred bioreactor. At 50 rpm agitation speed and 0.33 vvm aeration rate, 2.68 g/L (+)-terrein was achieved by feeding twofold concentrated maltose and glucose medium on the sixth day and controlling pH at 4.5 from the fourth day. This study lays foundation for the mass production of (+)-terrein by the marine filamentous A. terreus strain PF-26 in the stirred bioreactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

References

  1. Raistrick H, Smith G (1935) Studies in the biochemistry of micro-organisms: the metabolic products of Aspergillus terreus Thom. A new mould metabolic product-terrein. Biochem J 29:606–611

    CAS  Google Scholar 

  2. Ghisalberti EL, Narbey MJ, Rowland CY (1990) Metabolites of Aspergillus terreus antagonistic towards the take-all fungus. J Nat Prod 53:520–522

    Article  CAS  Google Scholar 

  3. Arakawa M, Someno T, Kawada M, Ikeda D (2008) A new terrein glucoside, a novel inhibitor of angiogenin secretion in tumor angiogenesis. J Antibiot (Tokyo) 61:442–448

    Article  CAS  Google Scholar 

  4. Park SH, Kim DS, Kim WG, Ryoo IJ, Lee DH, Huh CH, Youn SW, Yoo ID, Park KC (2004) Terrein: a new melanogenesis inhibitor and its mechanism. Cell Mol Life Sci 61:2878–2885

    Article  CAS  Google Scholar 

  5. Kim DS, Cho HJ, Lee HK, Lee WH, Park ES, Youn SW, Park KC (2007) Terrein, a fungal metabolite, inhibits the epidermal proliferation of skin equivalents. J Dermatol Sci 46:65–68

    Article  CAS  Google Scholar 

  6. Lee JC, Yu MK, Lee R, Lee YH, Jeon JG, Lee MH, Jhee EC, Yoo ID, Yi HK (2008) Terrein reduces pulpal inflammation in human dental pulp cells. J Endodont 34:433–437

    Article  Google Scholar 

  7. Lee YH, Lee NH, Bhattarai G, Oh YT, Yu MK, Yoo ID, Jhee EC, Yi HK (2010) Enhancement of osteoblast biocompatibility on titanium surface with Terrein treatment. Cell Biochem Funct 28:678–685

    Article  Google Scholar 

  8. Kolb HC, Martin H, Hoffmann R (1990) A total synthesis of racemic and optically active terrein (trans-4,5-dihydroxy-3-[(E)-1-propenyl]-2-cyclopenten-1-one). Tetrahedron Asymmetry 1:237–250

    Article  CAS  Google Scholar 

  9. Lee S, Kim WG, Kim E, Ryoo IJ, Lee HK, Kim JN, Jung SH, Yoo ID (2005) Synthesis and melanin biosynthesis inhibitory activity of (±)-terrein produced by Penicillium sp. 20135. Bioorg Med Chem Lett 15:471–473

    Article  CAS  Google Scholar 

  10. Phattanawasin P, Pojchanakom K, Sotanaphun U, Piyapolrungroj N, Zungsontiporn S (2007) Weed growth inhibitors from Aspergillus fischeri TISTR 3272. Nat Prod Res 21:1286–1291

    Article  CAS  Google Scholar 

  11. Bhadury P, Mohammad BT, Wright PC (2006) The current status of natural products from marine fungi and their potential as anti-infective agents. J Ind Microbiol Biotechnol 33:325–337

    Article  CAS  Google Scholar 

  12. Cai M, Zhou X, Lu J, Fan W, Niu C, Zhou J, Sun X, Kang L, Zhang Y (2011) Enhancing aspergiolide: a production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresour Technol 102:3584–3586

    Article  CAS  Google Scholar 

  13. Tang YJ, Zhang W, Zhong JJ (2009) Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and Ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum. Bioresour Technol 100:1852–1859

    Article  CAS  Google Scholar 

  14. Li H, Xu H, Li S, Feng X, Xu H, Ouyang P (2011) Effects of dissolved oxygen and shear stress on the synthesis and molecular weight of welan gum produced from Alcaligenes sp. CGMCC2428. Process Biochem 46:1172–1178

    Article  CAS  Google Scholar 

  15. Yin Y, Gao Q, Zhang F, Li Z (2012) Medium optimization for the high yield production of single (+)-terrein by Aspergillus terreus strain PF26 derived from marine sponge Phakellia fusca. Process Biochem 47:887–891

    Article  CAS  Google Scholar 

  16. Ding B, Yin Y, Zhang FL, Li ZY (2011) Recovery and phylogenetic diversity of culturable fungi associated with marine sponges Clathrina luteoculcitella and Holoxea sp. in the South China Sea. Mar Biotechnol (NY) 13:713–721

    Article  CAS  Google Scholar 

  17. Li ZY, Liu Y (2006) Marine sponge Craniella austrialiensis-associated bacterial diversity revelation based on 16S rDNA library and biologically active Actinomycetes screening, phylogenetic analysis. Lett Appl Microbiol 43:410–416

    Article  CAS  Google Scholar 

  18. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  19. Elibol M, Moreira AR (2005) Optimizing some factors affecting alkaline protease production by a marine bacterium Teredinobacter turnirae under solid substrate fermentation. Process Biochem 40:1951–1956

    Article  CAS  Google Scholar 

  20. Jüsten P, Paul GC, Nienow AW, Thomas C (1998) Dependence of penicillium chrysogenum growth, morphology, vacuolation, and productivity in fed-batch fermentations on impeller type and agitation intensity. Biotechnol Bioeng 59:762–775

    Article  Google Scholar 

  21. Gibbs PA, Seviour RJ, Schmid F (2000) Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol 20:17–48

    Article  CAS  Google Scholar 

  22. Zhou W, Holzhauer-Rieger K, Dors M, Schügerl K (1992) Influence of dissolved oxygen concentration on the biosynthesis of cephalosporin C. Enzyme Microb Technol 14:848–854

    Article  CAS  Google Scholar 

  23. Seo MJ, Kim MJ, Lee HH, Kim SR, Kang BW, Park JU, Rhu EJ, Choi YH, Jeong YK (2010) Initial acidic pH is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, Laetiporus sulphureus var. miniatus JM 27. J Microbiol 48:881–884

    Article  CAS  Google Scholar 

  24. Zou X, Guo X, Sun M (2009) pH Control strategy in a shaken minibioreactor for polysaccharide production by medicinal mushroom Phellinus linteus and its anti-hyperlipemia activity. Bioproc Biosyst Eng 32:277–281

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the High-Tech Research and Development Program of China (2011AA09070203).

Author information

Authors and Affiliations

  1. Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People’s Republic of China

    Bailiang Xu, Ying Yin, Fengli Zhang, Zhiyong Li & Lianyun Wang

Authors
  1. Bailiang Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ying Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fengli Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiyong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lianyun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zhiyong Li or Lianyun Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, B., Yin, Y., Zhang, F. et al. Operating conditions optimization for (+)-terrein production in a stirred bioreactor by Aspergillus terreus strain PF-26 from marine sponge Phakellia fusca . Bioprocess Biosyst Eng 35, 1651–1655 (2012). https://doi.org/10.1007/s00449-012-0735-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-012-0735-z

Keywords

Search

Navigation