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Foliar δ15N is affected by foliar nitrogen uptake, soil nitrogen, and mycorrhizae along a nitrogen deposition gradient

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Abstract

Foliar nitrogen isotope (δ15N) composition patterns have been linked to soil N, mycorrhizal fractionation, and within-plant fractionations. However, few studies have examined the potential importance of the direct foliar uptake of gaseous reactive N on foliar δ15N. Using an experimental set-up in which the rate of mycorrhizal infection was reduced using a fungicide, we examined the influence of mycorrhizae on foliar δ15N in potted red maple (Acer rubrum) seedlings along a regional N deposition gradient in New York State. Mycorrhizal associations altered foliar δ15N values in red maple seedlings from 0.06 to 0.74 ‰ across sites. At the same sites, we explored the predictive roles of direct foliar N uptake, soil δ15N, and mycorrhizae on foliar δ15N in adult stands of A. rubrum, American beech (Fagus grandifolia), black birch (Betula lenta), and red oak (Quercus rubra). Multiple regression analysis indicated that ambient atmospheric nitrogen dioxide (NO2) concentration explained 0, 69, 23, and 45 % of the variation in foliar δ15N in American beech, red maple, red oak, and black birch, respectively, after accounting for the influence of soil δ15N. There was no correlation between foliar δ13C and foliar %N with increasing atmospheric NO2 concentration in most species. Our findings suggest that total canopy uptake, and likely direct foliar N uptake, of pollution-derived atmospheric N deposition may significantly impact foliar δ15N in several dominant species occurring in temperate forest ecosystems.

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Acknowledgments

This research was funded by a Kieckhefer Adirondack Fellowship and by the Cornell NSF-IGERT in Biogeochemistry and Environmental Biocomplexity, Award #DGE 0221658 to D. Vallano. We thank J. Lavallae, R. Doyle-Morin, D. Lombardozzi, and K. Wagner for field support. G. Lovett provided invaluable guidance, A. Eller assisted in statistical analyses and J. Arcate helped initiate The New York Botanical Garden sampling. Special thanks to those who have assisted us with this project at the following sites: Adirondack Ecological Center, Fordham University’s Louis Calder Center, the Cary Institute for Ecosystem Studies, Mianus River Gorge Preserve, Clarence Fahnestock State Park, The New York Botanical Garden, and Inwood Hill Park.

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  1. Dena M. Vallano

    Present address: American Association for the Advancement of Science, 1200 New York Avenue Northwest, Washington, DC, 20005, USA

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  1. Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, 14853-2701, USA

    Dena M. Vallano & Jed P. Sparks

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Correspondence to Dena M. Vallano.

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Communicated by Hermann Heilmeier.

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Vallano, D.M., Sparks, J.P. Foliar δ15N is affected by foliar nitrogen uptake, soil nitrogen, and mycorrhizae along a nitrogen deposition gradient. Oecologia 172, 47–58 (2013). https://doi.org/10.1007/s00442-012-2489-3

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