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Two genes conferring resistance to Pythium stalk rot in maize inbred line Qi319

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Abstract

Stalk rots are destructive diseases in maize around the world, and are most often caused by the pathogen Pythium, Fusarium and other fungi. The most efficient management for controlling stalk rots is to breed resistant cultivars. Pythium stalk rot can cause serious yield loss on maize, and to find the resistance genes from the existing germplasm is the basis to develop Pythium-resistance hybrid lines. In this study, we investigated the genetic resistance to Pythium stalk rot in inbred line Qi319 using F2 and F2:3 population, and found that the resistance to Pythium inflatum in Qi319 was conferred by two independently inherited dominant genes, RpiQI319-1 and RpiQI319-2. Linkage analysis uncovered that the RpiQI319-1 co-segregated with markers bnlg1203, and bnlg2057 on chromosome 1, and that the RpiQI319-2 locus co-segregated with markers umc2069 and bnlg1716 on chromosome 10. The RpiQI319-1 locus was further mapped into a ~500-kb interval flanked by markers SSRZ33 and SSRZ47. These results will facilitate marker-assisted selection of Pythium stalk rot-resistant cultivars in maize breeding. To our knowledge, this is the first report on the resistance to P. inflatum in the inbred line Qi319, and is also the first description of two independently inherited dominant genes conferring the resistance of Pythium stalk rot in maize.

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Acknowledgments

We thank Hai Wang, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, for revision and helpful comments on the manuscript. This work was funded by the China Agriculture Research System (Maize) from the Ministry of Agriculture of China (CARS-02), the Public Welfare Special Fund from Institute of Crop Science, Chinese Academy of Agricultural Sciences (2060302-2-13), and the Agricultural Science and Technology Innovation Program from Chinese Academy of Agricultural Sciences.

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Authors and Affiliations

  1. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

    Feng-Jing Song, Can-Xing Duan, Hong-Jie Li, Zhen-Dong Zhu, Su-Li Sun, Xiao-Fei Wu & Xiao-Ming Wang

  2. Chinese Academy of Science, A Joint Research Center of Molecular Breeding for Northern Japonica Rice, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China

    Ming-Gang Xiao

  3. College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao, China

    Bao-Tao Liu

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  1. Feng-Jing Song
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  2. Ming-Gang Xiao
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  4. Hong-Jie Li
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  9. Xiao-Ming Wang
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Correspondence to Xiao-Ming Wang.

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Communicated by J. Lai.

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Song, FJ., Xiao, MG., Duan, CX. et al. Two genes conferring resistance to Pythium stalk rot in maize inbred line Qi319. Mol Genet Genomics 290, 1543–1549 (2015). https://doi.org/10.1007/s00438-015-1019-5

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  • DOI: https://doi.org/10.1007/s00438-015-1019-5

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