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Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat

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Abstract

Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A–5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4At–3At translocation.

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Acknowledgements

We thank W. John Raupp for editing of the manuscript, Duane Wilson for excellent technical assistance, and Dr. Sunish Sehgal for help with the sequencing. This research was supported by grants from the Kansas Wheat Commission and the Kansas Crop Improvement Association. This is contribution number 13-029-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, USA.

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  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    Tatiana V. Danilova, Bernd Friebe & Bikram S. Gill

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  1. Tatiana V. Danilova
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Correspondence to Bernd Friebe.

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Danilova, T.V., Friebe, B. & Gill, B.S. Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Chromosoma 121, 597–611 (2012). https://doi.org/10.1007/s00412-012-0384-7

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