Abstract
In this paper, a novel chitosan-g-(-O-methyl poly (ethylene glycol))-g-(-N-Tat peptide) (CS-mPEG-Tat) copolymer was synthesized. The synthesized intermediates and final products were characterized and confirmed by Fourier transform infrared spectrum, 1H nuclear magnetic resonance spectrum, and X-ray diffraction, respectively. The particle sizes, size distributions, and zeta potentials can also be determined by dynamic light scattering. Agarose gel electrophoresis study showed effective DNA-binding ability of CS-mPEG-Tat. In vitro cytotoxicity assay indicated that CS-mPEG-Tat copolymers were low toxic and cell compatible as the polymer concentration was smaller than 5 mg/ml. This work provides a facile approach to prepare biocompatible PEG-peptide-chitosan copolymer nanoparticles with controllable performances. In conclusion, the obtained CS-mPEG-Tat copolymer might be attractive cationic polymers for nonviral gene therapy.
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Acknowledgments
This research has received financial support from the National Natural Science Foundation of China (no. 81273471, 81001357 and 30901868), the Doctoral Program of Higher Education of China (no. 20090181120114), the Fundamental Research Funds for the Central Universities (no. 2010SCU23008), and the Science Program of the Health Office of Sichuan Province, China (no. 090342).
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Li, B., Ma, Q., He, G. et al. Synthesis and characterization of a novel methoxy poly(ethylene glycol)-Tat peptide-chitosan copolymers. Colloid Polym Sci 291, 1319–1327 (2013). https://doi.org/10.1007/s00396-012-2861-1
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DOI: https://doi.org/10.1007/s00396-012-2861-1