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Enzyme-based formulations for decontamination: current state and perspectives

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Abstract

Development of noncorrosive, cost-effective, environmentally benign, and broad-spectrum antimicrobial formulations is necessary for clinical, industrial, and domestic purposes. Many current decontaminating formulations are effective, but they require the use of strong oxidizing agents or organic solvents that have deleterious effects on human health and the surrounding environment. The emergence of antibiotic-resistant pathogens has motivated researchers to develop enzyme-based self-decontaminating formulations as alternatives to such chemical decontamination approaches. Hydrolytic and oxidative enzymes can be used to deactivate pathogens, including bacteria, spores, viruses, and fungi. Laccases, haloperoxidases, and perhydrolases catalyze the generation of biocidal oxidants, such as iodine, bromine, hypohalous acid (e.g., HOCl or HOBr), and peracetic acid. These oxidants have broad-spectrum antimicrobial activity. Due to the multi-pathway action of these oxidants, it has proven extremely difficult for microbes to gain resistance. Thus far, few examples have been reported on enzyme-based antimicrobial formulations. For these reasons, various enzyme-containing antimicrobial formulations are highlighted in this review.

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Acknowledgments

This work was supported by the U.S. Army Corps of Engineers under contracts W913T-10-2-0006 and W913T-11-R-0033.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Navdeep Grover, Ravi S. Kane & Jonathan S. Dordick

  2. Department of Chemical Engineering, Benjamin M. Statler College of Engineering and Mineral Resources, West Virginia University, Morgantown, WV, 26506, USA

    Cerasela Zoica Dinu

  3. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, 2213 Center for Biotechnology and Interdisciplinary Studies, 110 8th Street, Troy, NY, 12180, USA

    Jonathan S. Dordick

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  1. Navdeep Grover
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  2. Cerasela Zoica Dinu
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  3. Ravi S. Kane
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  4. Jonathan S. Dordick
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Correspondence to Jonathan S. Dordick.

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Grover, N., Dinu, C.Z., Kane, R.S. et al. Enzyme-based formulations for decontamination: current state and perspectives. Appl Microbiol Biotechnol 97, 3293–3300 (2013). https://doi.org/10.1007/s00253-013-4797-x

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