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Desmids and Biofilms of Freshwater Wetlands: Development and Microarchitecture

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Abstract

Freshwater wetlands constitute important ecosystems, and their benthic, attached microbial communities, including biofilms, represent key habitats that contribute to primary productivity, nutrient cycling, and substrate stabilization. In many wetland biofilms, algae constitute significant parts of the microbial population, yet little is known about their activities in these communities. An analysis of wetland biofilms from the Adirondack region of New York (USA) was performed with special emphasis on desmids, a group of evolutionarily advanced green algae commonly found in these habitats. Desmids constituted as much as 23.7% of the total algal and cyanobacterial flora of the biofilms during the July and August study periods. These algae represented some of the first eukaryotes to colonize new substrates, and during July their numbers correlated with fluctuations in general biofilm parameters such as biofilm thickness and dry weight as well as total carbohydrate. Significant numbers of bacteria were associated with both the EPS sheaths and cell wall surfaces of the desmids. Colonization of new substrates and development of biofilms were rapid and were followed by various fluctuations in microbial community structure over the short- and long-term observations. In addition to desmids, diatoms, filamentous green algae and transient non-motile phases of flagellates represented the photosynthetic eukaryotes of these biofilms.

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References

  1. Aubert, H, Brook, AJ, Shephard, KL (1989) Measurement of the adhesion of a desmid to a substrate. Br Phycol J 24: 293–295

    Article  Google Scholar 

  2. Baranguet, C, van Beusekom, SAM, Veuger, B, Neu, TR, Manders, EMM, Sinke, JJ, Admiraal, W (2004) Studying undisturbed autorophic biofilms: still a technical challenge. Aquat Microb Ecol 34: 1–9

    Article  Google Scholar 

  3. Baylson, FA, Stevens, BW, Domozych, DS (2001) Composition and synthesis of the pectin and protein components of the cell wall of Closterium acerosum (Chlorophyta). J Phycol 37: 796–809

    Article  CAS  Google Scholar 

  4. Ben-Ari, E (1999) Not just slime. BioScience 49: 689–695

    Article  Google Scholar 

  5. Bott, TL (1996) Algae in microscopic food webs. In: Stevenson, RJ, Bothwell, ML, Lowe, RL (Eds.) Algal Ecology: Freshwater Benthic Ecosystems. Academic Press, New York, pp 574–608

    Google Scholar 

  6. Brook, AJ (1981) The Biology of Desmids. Botanicals Monographs, vol 16. Blackwell Scientific Publications, Oxford, pp 276

    Google Scholar 

  7. Brook, AJ, Ellis, W (1987) The feeding of amoebae on desmids. Microscopy 35: 537–540

    Google Scholar 

  8. Browder, JA, Gleason, PJ, Swift, DR (1994) Periphyton in the Everglades: spatial variation, environmental correlates, and ecological implications. In: David, SM, Ogden, JC (Eds.) Everglades. The Ecosystem And Restoration. St. Lucie Press, Delray Beach, FL, pp 379–416

    Google Scholar 

  9. Burkholder, JM, Sheath, RG (1984) The seasonal distribution, abundance and diversity of desmids (Chlorophyta) in a softwater, north temperate stream. J Phycol 20: 159–172

    Article  Google Scholar 

  10. Coesel, PFM (1997) The edibility of Staurastrum chaetoceros and Cosmarium abbreviatum (Desmidiaceae) for Daphnia galeata/hyaline and the role of desmids in aquatic food chains. Aquat Ecol 31: 73–78

    Article  Google Scholar 

  11. Coesel, PFM (1994) On the ecological significance of a cellular mucilaginous envelope in planktic desmids. Algological Studies 73: 65–74

    Google Scholar 

  12. Costerton, JW (2000) Biofilms in the millennium: musings from a peak at Xanadu. In: Allison, DA, Gilbert, P, Lippin-Scott, HM, Wilson, M (Eds.) Community Structure and Cooperation in Biofilms. Cambridge University Press, New York, pp 329–344

    Google Scholar 

  13. Davey, ME, O’Toole, GA (2000) Microbial biofilm from ecology to molecular genetics. Microbiol Mol Biol Rev 64: 847–867

    Article  PubMed  CAS  Google Scholar 

  14. Devito, KJ, Dillon, P (1993) Importance of runoff and winter anoxia to the P and N dynamics of a beaver pond. Can J Fish Aquat Sci 50: 2222–2234

    Article  CAS  Google Scholar 

  15. Devito, KJ, Hill, AR (1997) Sulphate dynamics in relation to groundwater-surface water interactions in headwater wetlands of the southern Canadian Shield. Hydrol Process 11: 485–500

    Article  Google Scholar 

  16. Dodds, WK (2002) Freshwater ecology: concepts and environmental applications. Academic Press, San Diego, pp 569

    Google Scholar 

  17. Domozych, DS, Kort, S, Benton, S, Yu, T (2005) The extracellular polymeric substance of the green alga Penium margaritaceum and its role in biofilm formation. Biofilms 2: 1–16

    Article  Google Scholar 

  18. Domozych, CR, Plante K, Blais, P, Paliulis, L, Domozych, DS (1993) Mucilage processing and secretion in the green alga Closterium. I. Cytology and biochemistry. J Phycol 29: 650–659

    Article  Google Scholar 

  19. Domozych, DS, Domozych, CR (1993) Mucilage processing and secretion in the green alga Closterium. II. Ultrastructure and immunochemistry. J Phycol 29: 659–667

    Article  Google Scholar 

  20. Dubois, M, Gilles, KA, Hamilton, JK, Rebers, PA, Smith, F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356

    Article  CAS  Google Scholar 

  21. Edgar, LA, Pickett-Heaps, JD (1984) Diatom locomotion. In: Round, FE, Chapman, DJ (Eds.) Progress in Phycological Research. Vol. 3. Biopress Ltd. Bristol, UK, pp 47–88

    Google Scholar 

  22. Fisher, MM, Wilcox, LW (1996) Desmid–bacterial associations in Sphagnum-dominated Wisconsin peatlands. J Phycol 32: 543–549

    Article  Google Scholar 

  23. Fisher, MM, Wilcox, LW, Graham, LE (1998a) Molecular characterization of epiphyte bacterial communities on charophycean green algae. Appl Environ Microbiol 64: 4384–4389

    PubMed  CAS  Google Scholar 

  24. Fisher, MM, Wilcox, LW, Graham, LE (1998b) Bacterial numbers and activities in two northern Wisconsin Sphagnum bogs. Microb Ecol 36: 259–269

    Article  PubMed  Google Scholar 

  25. Gaur, JP, Rai, LC (2001) Heavy metal tolerance in algae. In: Rai, LC, Gaur, JP (Eds.) Algal Adaptation to Environmental Stresses. Springer, New York, pp 363–388

    Google Scholar 

  26. Gerrath, JF (2003) Conjugating green algae and desmids. In: Wehr, JD, Sheath, RG (Eds.) Freshwater Algae of North America. Ecology and Classification Academic Press, New York, pp 353–381

    Google Scholar 

  27. Gerrath, JF (1993) The biology of desmids: a decade of progress. In: Round, FE, Chapman, DJ (Eds.) Progress in Phycological Research, Vol. 9, Biopress, Bristol, pp 79–192

    Google Scholar 

  28. Goldsborough, LG, Robinson, GGC (1996) Pattern in wetlands. In: Stevenson, RJ, Bothwell, ML, Lowe, RL (Eds.) Algal Ecology: Freshwater Benthic Ecosystems. Academic Press, New York, pp 353–381

    Google Scholar 

  29. Graham, LE, Graham, JM, Wilcox, LW (2006) Plant Biology. Pearson/Prentice-Hall, Upper Saddle River, New Jersey, pp 670

    Google Scholar 

  30. Graham, LE, Wilcox, LW (2000) Algae. Prentice Hall, New Jersery, pp 640

    Google Scholar 

  31. Hader, DP (1982) Effects of inhibitors on photomovement in desmids. Arch Microbiol 129: 168–172

    Article  Google Scholar 

  32. Hader, DP (1981) Coupling of photomovement and photosynthesis in desmids. Cell Motil 2: 73–82

    Article  Google Scholar 

  33. Hader, DP, Wenderoth, K (1986) Light induced electrical potential changes and motility in desmids. Gen Phys Biophys 5: 173–178

    CAS  Google Scholar 

  34. Hoagland, KD, Rosowski, JR, Gretz, MR, Roemer, SC (1993) Diatom extracellular polymeric substrates: function, fine structure, chemistry, and physiology. J Phycol 29: 537–566

    Article  CAS  Google Scholar 

  35. Jensen, A (1978) Chlorophyll and carotenoids. In: Hellebust, JA, Craigie, JS (Eds.) Handbook of Phycological Methods. Physiological and Biochemical Methods. Cambridge University Press, Cambridge, UK

    Google Scholar 

  36. Kalff, J (2002) Limnology. Inland water ecosystems. Prentice-Hall, Upper Saddle River, New Jersey, pp 592

    Google Scholar 

  37. Keevil, CW, Godfree, A, Holt, D, Dow, C (1999) Biofilms in the aquatic environment. Royal Society of Chemistry, Cambridge, UK, pp 242

    Google Scholar 

  38. Klarer, DM, Millie, DF (1992) Aquatic macrophytes and algae at Old Woman Creek estuary and other great lakes coastal wetlands. J Great Lakes Res 18: 6322–633

    Article  Google Scholar 

  39. Komarek, J (2003) Coccoid and colonial cyanobacteria. In: Wehr, JD, Sheath, RE (Eds.) Freshwater Algae of North America. Elsevier Science, New York, pp 59–116

    Google Scholar 

  40. Lamberti, GA (1996) The role of periphyton in benthic food webs. In: Stevenson, RJ, Bothwell, ML, Lowe, RL (Eds.) Algal Ecology: Freshwater Benthic Ecosystems. Academic Press, New York, pp 533–573

    Google Scholar 

  41. Ledger, ME, Hildrew, AG (1998) Temporal and spatial variation in the epilithic biofilm of an acid stream. Freshw Biol 40: 655–670

    Article  Google Scholar 

  42. Leppard, GG (1995) The characterization of algal and microbial mucilages and their aggregates in aquatic ecosystems. Sci Total Environ 165: 103–121

    Article  PubMed  CAS  Google Scholar 

  43. Linde, J, Morse, L, Domozych, DS (2001) Calmodulin and its role in the secretory apparatus of the desmid Closterium. Int J Plant Sci 162: 15–27

    Article  CAS  Google Scholar 

  44. Lorch, D (1986) Desmids and heavy metals. I. Uptake of lead by cultures and isolated cell walls of selected species. Beiheft zur Nova Hedwigia 56: 105–118

    Google Scholar 

  45. Lorch, D (1978) Manganese: uptake and influence on growth and morphogenesis of selected species. Arch Hydrobiol 84: 166–179

    CAS  Google Scholar 

  46. McCormick, PV, O’Dell, MB, Shuford III, RBE, Backus, JG, Kennedy, WC (2001) Periphyton responses to experimental phosphorus enrichment in a subtropical wetland. Aquat Bot 71: 119–139

    Article  CAS  Google Scholar 

  47. McCormick, PV, Shuford, RBE, Backus, JG, Kennedy, WC (1998) Spatial and seasonal patterns of periphyton biomass and productivity in the northern Everglades, Florida, USA. Hydrobiologia 362: 185–208

    Article  Google Scholar 

  48. McCormick, PV, Rawlik, PS, Lurding, K, Smith, EP, Sklar, FH (1996) Periphyton–water quality relationships along a nutrient gradient in the northern Florida Everglades. J North Am Benthol Soc 15: 433–449

    Article  Google Scholar 

  49. Mitsch, WJ, Gosselink, JG (1993) Wetlands. Van Norstrand-Reinhold, New York

    Google Scholar 

  50. Muller-Schwarze, D, Sun, L (2003) The Beaver: Natural History of a Wetlands Engineer. Cornell University Press, Ithaca, New York

    Google Scholar 

  51. Naiman, RJ, Johnston, CA, Kelly, JC (1988) Alteration of North American streams by beaver. BioScience 38: 753–762

    Article  Google Scholar 

  52. Neu, TR, Woefl, S, Lawrence, JR (2004) Three-dimensional differentiation of photo-autotrophic biofilm constituents by multi-channel laser scanning microscopy (single-photon and two-photon excitation). J Microbiol Methods 56: 161–172

    Article  PubMed  CAS  Google Scholar 

  53. Neu, TR, Lawrence, JR (1997) Development and structure of microbial biofilms in river water studied by confocal laser scanning microscopy. FEMS Microb Ecol 24: 11–25

    Article  CAS  Google Scholar 

  54. Oertel, A, Aichinger, N, Hochreiter, R, Thalhamer, J, Lutz-Meindl, U (2004) Analysis of mucilage secretion and excretion in Micrasterias (Chlorophyta) by means of immunoelectron microscopy and digital time lapse photography. J Phycol 40: 711–720

    Article  Google Scholar 

  55. Parsek, MR, Fuqua, C (2003) Biofilms 2003: emerging themes and challenges in studies of surface-associated microbial life. J Bacteriol 186: 4427–4440

    Article  CAS  Google Scholar 

  56. Paulsen, BS, Vieira, AAH (1994) Structure of the capsular and extracellular polysaccharides produced by the desmid Spondylosium panduriforme (Chlorophyta). J Phycol 30: 638–641

    Article  CAS  Google Scholar 

  57. Prescott, GW, Bicudo, C, Vinyard, WC (1982) A synopsis of North American desmids. Part II. Desmidicaceae: Placodermae. Section 4. University of Nebraska Press, Lincoln, pp 700

    Google Scholar 

  58. Prescott, GW, Croasdale, HT, Vinyard, WC, Bicudo, C (1981) A synopsis of North American desmids. Part II. Desmidicaceae: Placodermae. Section 3. University of Nebraska Press, Lincoln, pp 720

    Google Scholar 

  59. Prescott, GW, Croasdale, HT, Vinyard, WC (1977) A synopsis of North American desmids. Part II. Desmidicaceae: Placodermae. Section 2. University of Nebraska Press, Lincoln, pp 413

    Google Scholar 

  60. Prescott, GW, Croasdale, HT, Vinyard, WC (1975) A synopsis of North American desmids. Part II. Desmidicaceae: Placodermae. Section 1. University of Nebraska Press, Lincoln, pp 275

    Google Scholar 

  61. Prescott, GW, Croasdale, HT, Vinyard, WC (1972) Desmidiales, Part 1. Saccodermae, Mesotaeniaceae. North American flora, Vol. II, Chap. 6, of Nebraska Press, Lincoln, p 84

  62. Rolaufs, P, Hering, D, Lohse, S (2001) Composition, invertebrate community and productivity of a beaver dam in comparison to other stream habitat types. Hydrobiologia 459: 201–212

    Article  Google Scholar 

  63. Shannon, CE, Weaver, W (1963) The Mathematical Theory of Communication 2University of Illinois Press, Urbana, pp 226

    Google Scholar 

  64. Spijkerman, E, Coesel, PFM (1998) Alkaline phosphatase activity in two planktonic desmid species and the possible role of an extracellular envelope. Freshw Biol 39: 503–513

    Article  CAS  Google Scholar 

  65. Spijkerman, E, Coesel, PFM (1997) Growth kinetic parameters of two planktonic desmid species under fluctuating phosphorus conditions in continuous flow culture. J Plank Res 19: 1899–1912

    Article  CAS  Google Scholar 

  66. Sutherland, IW (2001a) The biofilm matrix—an immobilized but dynamic microbial environment. Trends Microbiol 9: 222–227

    Article  PubMed  CAS  Google Scholar 

  67. Sutherland, IW (2001b) Biofilm exopolysaccahrides: a strong and sticky framework. Microbiology 147: 3–9

    PubMed  CAS  Google Scholar 

  68. Updegraff, K, Pastor, J, Bridgham, SD, Johnston, CA (1995) Environmental and substrate controls over carbon and nitrogen wetlands. Ecol Appl 5: 151–163

    Article  Google Scholar 

  69. Vicre, M, Santaella, C, Blanchet, S, Gateau, A, Driouich, A (2005) Root border-like cells of Arabidopsis. Microscopical characterization and role in the interaction with Rhizobacteria. Plant Physiol 138: 998–1008

    Article  PubMed  CAS  Google Scholar 

  70. Watanabe, MM, Mayama, S, Hiroki, M, Nozaki, H (2000) Biomass, species composition and diversity of epipelic algae in mire pools. Hydrobiologia 421: 91–102

    Article  Google Scholar 

  71. Watnick, P, Kilter, R (2000) Biofilm, city of microbes. J Bacteriol 182: 2675–2679

    Article  PubMed  CAS  Google Scholar 

  72. Wehr, JD (2003) Freshwater habitats of algae. In: Wehr, JD, Sheath, RG (Eds.) Freshwater Algae of North America. Ecology and Classification. Academic Press, New York, pp 11–57

    Google Scholar 

  73. Wimpenny, JWT (2000) An overview of biofilms as function communities. In: Allison, DG (Ed.) SGM Symposium 59: Community Structure and Cooperation in Biofilms. Cambridge University Press, Cambridge, UK, pp 1–24

    Google Scholar 

  74. Woelkerling, WJ, Gough, SB (1976) Wisconsin desmids. I. Aufwuchs and plankton communities of selected acid bogs, alkaline bogs, and closed bogs. Hydrobiologia 48: 209–232

    Article  Google Scholar 

  75. Woelkerling, WJ, Gough, SB (1976) Wisconsin desmids. III. Desmid community composition and distribution in relation to lake type and water chemistry. Hydrobiologia 51: 3–11

    Article  Google Scholar 

  76. Wright, JP, Jones, CG, Flecker, AS (2002) An ecosystem engineer, the beaver, increase in species richness at the landscape level. Oecologia 132: 96–101

    Article  Google Scholar 

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Acknowledgements

Special thanks to Kelly Dempsey-Little for her help in this project. Part of this study was supported by NSF grant 0419131 and Faculty Development Grants from Skidmore College.

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  1. Department of Biology, Skidmore College, 815 North Broadway, Saratoga Springs, New York, 12866, USA

    David S. Domozych & Catherine Rogers Domozych

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Domozych, D.S., Domozych, C.R. Desmids and Biofilms of Freshwater Wetlands: Development and Microarchitecture. Microb Ecol 55, 81–93 (2008). https://doi.org/10.1007/s00248-007-9253-y

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