Abstract
The majority of ecotoxicological data are generated from standard laboratory-based experiments with organisms exposed in nonflowing systems using highly purified water, which contains very low amounts of dissolved organic matter and suspended particulates. However, such experimental conditions are not ecologically relevant. Thus, there is a need to develop more realistic approaches to determining toxicity, including both lethal and sublethal effects. This research provides information on the effect of natural water constituents, such as suspended particulates and dissolved organic matter, in river water (RW) on the chronic toxicity (7-day reproductive impairment) of the pesticides atrazine, chlorothalonil, and permethrin to the freshwater cladoceran Ceriodaphnia cf. dubia. Standard bioassays were conducted under standard laboratory and more environmentally realistic conditions (using RW). The 7-day IC25 (reproduction impairment) values of atrazine, chlorothalonil, and permethrin to C. cf. dubia ranged from 862.4 to >1000, 51.3 to 66.4, and 0.19 to 0.23 μg/L, respectively. Using the Globally Harmonized System of Classification and Labelling of Chemicals, atrazine is classified as moderately to highly toxic, whereas permethrin and chlorothalonil were both highly toxic. The presence of dissolved organic matter and suspended particles in natural RW did not significantly (p > 0.05) change the toxicity of any of the pesticides to C. cf. dubia compared with that tested in laboratory water (LW). For the tested pesticides, toxicity testing in LW provided an adequate estimate of the hazard posed.
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References
Akkanen J, Penttinen S, Haitzer M, Kukkonen JVK (2001) Bioavailability of atrazine, pyrene and benzo [a] pyrene in European river waters. Chemosphere 45:453–462
Baker EK et al (1996) Ecological and human health risk assessment of chemicals in sewage treatment plant dischages to the Hawkesbury Nepean River. Sydney Water Corporation, p 145
Bauld J, Evans W, Sandstorm M (1992) Groundwater quality under irrigated agriculture: Murray Basin, Southeastern Australia. In: International workshop on groundwater and environment Beijing, China Seismological Press, Beijing, pp 447–457
Bejarano AC, Chandler GT, Decho AW (2005) Influence of natural dissolved organic matter (DOM) on acute and chronic toxicity of the pesticides chlorothalonil, chlorpyrifos and fipronil on the meiobenthic estuarine copepod Amphiascus tenuiremis. J Exp Mar Biol Ecol 321:43–57
Birch G, Shotter N, Steetsel P (1998) The environmental status of Hawkesbury River sediments. Aust Geogr Stud 36:37–57
Bowmer KH, Korth W, Scott A, McCorkelle G, Thomas M (1998) Pesticide monitoring in the irrigation areas of south-western NSW, 1990–1995. Technical Report 17/98. CSIRO Land and Water, Australia, p 156
Caux PY, Kent RA, Fan GT, Stephenson GL (1996) Environmental fate and effects of chlorothalonil: a Canadian perspective. Crit Rev Environ Sci Technol 26:45–93
Clark JM, Brooks GM (1989) Neurotoxicology of pyrethroids: single or multiple mechanisms of action. Environ Toxicol Chem 8:361–372
Clark JM, Matsumura F (1982) Two different types of inhibitory effects of pyrethroids on nerve Ca− and Ca+ Mg ATpase in the squid, Loligo paelei. Pestic Biochem Physiol 4:232–238
Coats J, Symonik D, Bradbury S, Dyer S, Timson L, Atchison G (1989) Toxicology of synthetic pyrethroids in aquatic organisms: an overview. Environ Toxicol Chem 8:671–679
Davies PE, Cook LS, Barton JL (1994) Triazine herbicide contamination of Tasmanian streams: sources, concentrations and effects on biota. Aust J Mar Freshwater Res 45:209–226
DeLorenzo ME, Serrano L (2003) Individual and mixture toxicity of three pesticides, atrazine, chlorpyrifos, and chlorothalonil, to the marine phytoplankton species Dunaliella tertiolecta. J Environ Sci Health B B38:529–538
DeLorenzo ME, Serrano L, Chung KW, Hoguet J, Key PB (2006) Effects of the insecticide permethrin on three life stages of the grass shrimp, Palaemonetes pugio. Ecotoxicol Environ Saf 64:122–127
Forbes TL, Forbes VE (1993) Critique of the use of distribution-based extrapolation models in ecotoxicology. Funct Ecol 7:249–254
Forney DR, Davis DE (1981) Effects of low concentrations of herbicides on submersed aquatic plants. Weed Sci 29:677–685
Globally harmonized system of classification and labelling of chemicals (2009) Third revised edition. ST/SG/AC.10/30/Rev.3. United Nations, New York
Godard T, Fessard V, Huet S, Mourot A, Deslandes E, Pottier D et al (1999) Comparative in vitro and in vivo assessment of genotoxic effects of etoposide and chlorothalonil by the comet assay. Mutat Res 444:103–116
Gulley D, Boelter A, Bergman H (1991) Toxstat 3.3. Fish Physiology and Toxicology Laboratory, University of Wyoming, Laramie
Haitzer M, Hoss S, Traunspurger W, Steinberg C (1998) Effects of dissolved organic matter (DOM) on the bioconcentration of organic chemicals in aquatic organisms: a review. Chemosphere 37:1335–1362
Imgrund H (2003) Environmental fate of permethrin. California Department of Pesticide Regulation, Environmental Monitoring Branch, Sacramento
Julli M, Chapman J, Thompson GT (1990) Use of Australian cladocerans to generate life-cycle toxicity data. Environ Monit Assess 14:353–362
Kefford BJ, Palmer CG, Jooste S, Warne MS, Nugegoda D (2005) What is meant by 95 % of species? An argument for the inclusion of rapid tolerance testing. Hum Ecol Risk Assess 11:1025–1046
Leonard A, Hyne R, Lim R, Chapman J (1999) Effect of endosulfan runoff from cotton fields on macroinvertebrates in the Namoi River. Ecotoxicol Environ Saf 42:125–134
Mueller-Beilschmidt D (1990) Toxicology and environmental fate of synthetic pyrethroids. J pesticide reform 10. www.mindfully.org/Pesticide/Pyrethroids-Synthetic.htm. Accessed 21 Nov 2012
Muschal M (2000) Central and North West Regions Water Quality Program—1997/98 report on pesticide monitoring. CNR20000.004. NSW Department of Land Water Conservation, Newcastle
Muschal M (2001) Central and northwest regions water quality program (CNWRWQP)—1999/2000 report on pesticide monitoring. NSW Department of Land and Water Conservation, Parramatta
New South Wales Department of Environment and Planning (1983) Lower Colo studies: the biological and physical consequences of river sand extraction. A joint report prepared for Colo River action group. Hawkesbury Nepean valley report. NSW Department of Environment and Planning, Sydney
New South Wales Environmental Protection Agency (1998) Screening for volatile and semi-volatile organic compounds in wastes, soils, sediments and waters. Organic chemistry unit methods manual, NSW EPA, Sydney
Nikkila A, Paulasson M, Almgren K, Blankck H, Kukkonen JVK (2001) Atrazine uptake, elimination, and bio-concentration by periphyton communities and Daphnia magna: effects of dissolved organic carbon. Environ Toxicol Chem 20:1003–1011
Oris JT, Winner RW, Moore MV (1991) A four-day survival and reproduction toxicity test for Ceriodaphnia dubia. Environ Toxicol Chem 10:217–224
Phyu YL, Warne MSJ, Lim RP (2004) Toxicity of atrazine and molinate to the cladoceran Daphnia carinata and the effect of river water and bottom sediment on their bioavailability. Arch Environ Contam Toxicol 46:308–315
Phyu YL, Warne MSJ, Lim RP (2005a) Effect of river water, sediment and time on the toxicity and bioavailability of atrazine and molinate to the marine bacterim Vibrio fischeri (Microtox). Water Res 39:2738–2746
Phyu YL, Warne MSJ, Lim RP (2005b) The toxicity and bioavailability of atrazine and molinate to Chironomus tepperi larvae in laboratory and river water in the presence and absence of sediment. Chemosphere 58:1231–1239
Phyu YL, Warne MSJ, Lim RP (2005c) Toxicity and bioavailability of atrazine and molinate to the fresh water shrimp (Paratya australiensis) under laboratory and simulated field conditions. Ecotoxicol Environ Saf 60:113–122
Phyu YL, Warne MSJ, Lim RP (2006) Toxicity and bioavailability of atrazine and molinate to a freshwater fish (Melanotenia fluviatilis) under laboratory and simulated field conditions. Sci Total Environ 356:86–99
Phyu YL, Warne MSJ, Lim RP (2008) Assessing the biological relevance of exposing freshwater organisms to atrazine and molinate in environmentally realistic exposure test systems. Environ Toxicol Chem 27:420–424
Ray LE, Murray HE, Giam GS (1983) Organic pollutants in marine samples from Portland, Maine. Chemosphere 12:1031–1038
Reed K, Mereish K, Jensen B (1984) Quantitative relationships between structure and pharmokinetic parameters using molecular connectivity chi indices. 1. Substituted2-sulfapyridines. J Pharm Sci 73:237–240
Ritz C, Streibig J (2005) Bioassay analysis using R. J Stat Softw 12:1–22
Solomon KR, Baker DB, Richards RP, Dixon KR, Klaine SJ, La Point TW et al (1996) Ecological risk assessment of atrazine in North American surface waters. Environ Toxicol Chem 15:31–76
Sprague JB, Fogels A (1977) Watch the Y in bioassay. Proceedings of the 3rd aquatic toxicity workshop, Halifax, Nov. 2–3, 1976. Environmental Protection Service Technical Report, EPS-5-AR-77-1. Halifax, pp 107–118
Stamer JK, Zelt RB (1994) Organonitrogen herbicides in the lower Kansas River Basin. J Am Water Works Assoc 86:93–104
Stratton GW, Corke C (1981) Interaction of permethrin with Daphnia magna in the presence and absence of particulate material. Environ Pollut 24:135–144
Tomlin C (2007) The pesticide manual: a world compendium (14th ed). British Crop Protection Council and Royal Society of Chemistry, Bath
United States Environmental Protection Agency (1994) Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms (3rd edn). USEPA/600/4-91/002
Van Leuwen CJ, Hermens JLM (1995) Risk assessment of chemicals: an introduction. Kluwer, Dordrecht
Waterman TH (1961) Comparative physiology. In: Waterman TH (ed) The physiology of Crustacea, Academic Press, New York
Wheelock CE, Miller MJ, Phillips BM, Gee SJ, Jeerdema RS, Hammock BD (2005) Influence of container adsorption upon observed pyrethroid toxicity to Ceriodaphnia dubia and Hyalella azteca. Aquat Toxicol 74:47–52
Yang W, Spurlock R, Liu W, Gan J (2006) Inhibition of aquatic toxicity of pyrethroid insecticides by suspended sediment. Environ Toxicol Chem 25:1913–1919
Zhang H, Clapp C, Mingelgrin U, Koskinen W, Owdy R (1990) Complexation of atrazine by humic acid. American Society of Agronomics, San AntoAbstracts, Annual Meetings, San Antonioio
Acknowledgments
The authors gratefully acknowledge the Australian Research Council for research funding; the Institute for Water and Environmental and Resource Management for research support and laboratory facilities; and the Centre of Ecotoxicology, Ecotoxicology & Environmental Contaminants Section, Office of Environment & Heritage, for use of its laboratory facilities at Lidcombe.
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Phyu, Y.L., Palmer, C.G., Warne, M.S.J. et al. Assessing the Chronic Toxicity of Atrazine, Permethrin, and Chlorothalonil to the Cladoceran Ceriodaphnia cf. dubia in Laboratory and Natural River Water. Arch Environ Contam Toxicol 64, 419–426 (2013). https://doi.org/10.1007/s00244-012-9837-5
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DOI: https://doi.org/10.1007/s00244-012-9837-5