Abstract
A method for the simultaneous analysis of nucleosides and nucleotides in infant formula using reversed-phase liquid chromatography–tandem mass spectrometry is described. This approach is advantageous for compliance testing of infant formula over other LC-MS methods in which only nucleotides or nucleosides are measured. Following sample dissolution, protein was removed by centrifugal ultrafiltration. Chromatographic analyses were performed using a C18 stationary phase and gradient elution of an ammonium acetate/bicarbonate buffer, mass spectrometric detection and quantitation by a stable isotope-labelled internal standard technique. A single laboratory validation was performed, with spike recoveries of 80.1–112.9 % and repeatability relative standard deviations of 1.9–7.2 %. Accuracy as bias was demonstrated against reference values for NIST1849a certified reference material. The method has been validated for the analysis of bovine milk-based, soy-based, caprine milk-based and hydrolysed milk protein-based infant formulae.
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The financial assistance of Fonterra Co-operative Group Limited and the Tertiary Education Commission in providing an Enterprise Scholarship is gratefully acknowledged.
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Gill, B.D., Indyk, H.E. & Manley-Harris, M. Analysis of nucleosides and nucleotides in infant formula by liquid chromatography–tandem mass spectrometry. Anal Bioanal Chem 405, 5311–5319 (2013). https://doi.org/10.1007/s00216-013-6935-9
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DOI: https://doi.org/10.1007/s00216-013-6935-9