Abstract
In this paper we describe a novel optical fiber-based technology for analyzing cell migration. Cells were labeled with a membrane-bound fluorescent dye and distributed onto a polished optical fiber bundle. When a cell passes over one of the individual fibers in the bundle, the membrane-bound dye causes a large intensity increase, which stays for a given “residence time” until the cell departs from the fiber. Residence time increases significantly upon exposure to an antimigratory drug, indicating a decrease in cell migration. This approach provides a simple migration assay and does not require sophisticated tracking software. By using optical fiber bundles containing smaller individual fibers with higher spatial resolution, this approach was employed to develop a migration assay based on subcellular imaging. The subcellular imaging platform allows for rapid analysis of migratory potential, reducing experimental time from several hours in a standard assay to 5 min using this technology.
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References
Price JT, Bonovich MT, Kohn EC (1997) Crit Rev Biochem Mol Biol 32:175–253
Bolognese A, Correale G, Manfra M, Lavecchia A, Mazzoni O, Novellino E, Barone V, Pani A, Tramontano E, La Colla P, Murgioni C, Serra I, Setzu G, Loddo R (2002) J Med Chem 45:5205–5216
Rosenberg B (1980) In: Sprio T (ed) Nucleic acid–metal ion interactions, vol 1. Wiley, New York, pp 1–29
Boyden S (1962) J Exp Med 115:453–466
Yamaguchi J, Kusano KF, Masuo O, Kawamoto A, Silver M, Murasawa S, Bosch-Marce M, Masuda H, Losordo DW, Isner JM, Asahara T (2003) Circulation 107:1322–1328
Magdalena J, Millard TH, Machesky LM (2003) J Cell Sci 116:743–756
Liao GJ, Nagasaki T, Gundersen GG (1995) J Cell Sci 108:3473–3483
Dertinger SKW, Chiu DT, Jeon NL, Whitesides GM (2001) Anal Chem 73:1240–1246
Wegener J, Keese CR, Giaever I (2000) Exp Cell Res 259:158–166
Deacon SW, Serpinskaya AS, Vaughan PS, Fanarraga ML, Vernos I, Vaughan KT, Gelfand VI (2003) J Cell Biol 160:297–301
Mrksich M, Dike LE, Tien J, Ingber DE, Whitesides GM (1997) Exp Cell Res 235:305–313
Yousaf MN, Houseman BT, Mrksich M (2001) Angew Chem Int Edit 40:1093–1096
Chon JH, Vizena AD, Rock BM, Chaikof EL (1997) Anal Biochem 252:246–254
Fisher PR, Merkl R, Gerisch G (1989) J Cell Biol 108:973–984
Hoebeke J, Vannijen G, Debrabander M (1976) Biochem Biophys Res Commun 69:319–324
Debrabander MJ, Vandeveire RML, Aerts FEM, Borgers M, Janssen PAJ (1976) Cancer Res 36:905–916
Schliwa M, Honer B (1993) Trends Cell Biol 3:377–380
Vasiliev JM, Gelfand IM, Domnina LV, Ivanova OY, Komm SG, Olshevsk LV (1970) J Embryol Exp Morph 24:625–640
Goldman RD (1971) J Cell Biol 51:752–762
Terasaki M, Chen LB, Fujiwara K (1986) J Cell Biol 103:1557–1568
Heggeness MH, Simon M, Singer SJ (1978) P Natl Acad Sci USA 75:3863–3866
Collot M, Louvard D, Singer SJ (1984) P Natl Acad Sci USA Biol 81:788–792
Freshney R (1994) Culture of animal cells: a nanual of basic techniques. Wiley, New York
Gilchrest BA, Nemore RE, Maciag T (1980) Cell Biol Int Rep 4:1009–1016
Chigusa Y, Fujiwara K, Hattori Y, Matsuda Y (1986) Optoelectron-Devices 1:203–216
Mogi M, Yoshimura K (1989) P Soc Photo-Opt Inst 1067:172–181
Walt DR (1998) Acc Chem Res 31:267–278
Honig MG, Hume RI (1986) J Cell Biol 103:171–187
Parish CR (1999) Immunol Cell Biol 77:499–508
Elowitz MB, Levine AJ, Siggia ED, Swain PS (2002) Science 297:1183–1186
Mukherjee S, Soe TT, Maxfield FR (1999) J Cell Biol 144:1271–1284
Vale RD, Schnapp BJ, Reese TS, Sheetz MP (1985) Cell 40:449–454
Debrabander M, Nuydens R, Geerts H, Hopkins CR (1988) Cell Motil Cytoskel 9:30–47
Herr FB, Heath MC (1982) Exp Mycol 6:15–24
Grigoriev IS, Chernobelskaya AA, Vorobjev IA (1999) Biol Membrany 16:21–41
Matteoni R, Kreis TE (1987) J Cell Biol 105:1253–1265
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This initial work was supported by a grant from the National Institutes of Health, Institute of General Medical Sciences.
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DiCesare, C., Biran, I. & Walt, D.R. Individual cell migration analysis using fiber-optic bundles. Anal Bioanal Chem 382, 37–43 (2005). https://doi.org/10.1007/s00216-005-3142-3
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DOI: https://doi.org/10.1007/s00216-005-3142-3