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Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation

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Abstract

Summary

Green tea polyphenols (GTP) are promising agents for preventing bone loss. GTP supplementation sustained microarchitecture and improved bone quality via a decrease in inflammation. Findings suggest a significant role for GTP in skeletal health of patients with chronic inflammation.

Introduction

This study evaluated whether GTP can restore bone microstructure along with a molecular mechanism in rats with chronic inflammation. A 2 [placebo vs. lipopolysaccharide (LPS)]× 2 [no GTP vs. 0.5% GTP (w/v) in drinking water] factorial design was employed.

Methods

Female rats were assigned to four groups: placebo, LPS, placebo + GTP, and LPS + GTP for 12 weeks. Efficacy was evaluated by examining changes in bone microarchitecture using histomorphometric and microcomputed tomographic analyses and by bone strength using the three-point bending test. A possible mechanism was studied by assessing the difference in tumor necrosis factor-α (TNF-α) expression in tibia using immunohistochemistry.

Results

LPS lowered trabecular volume fraction, thickness, and bone formation in proximal tibia while increasing osteoclast number and surface perimeter in proximal tibia and eroded surface in endocortical tibial shafts. GTP increased trabecular volume fraction and number in both femur and tibia and periosteal bone formation rate in tibial shafts while decreasing trabecular separation in proximal tibia and eroded surface in endocortical tibial shafts. There was an interaction between LPS and GTP in trabecular number, separation, bone formation, and osteoclast number in proximal tibia, and trabecular thickness and number in femur. GTP improved the strength of femur, while suppressing TNF-α expression in tibia.

Conclusion

In conclusion, GTP supplementation mitigated deterioration of bone microarchitecture and improved bone integrity in rats with chronic inflammation by suppressing bone erosion and modulating cancellous and endocortical bone compartments, resulting in a larger net bone volume. Such a protective role of GTP may be due to a suppression of TNF-α.

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Acknowledgements

This study was supported by the Laura W. Bush Institute for Women’s Health and National Institutes of Health/National Center for Complementary and Alternative Medicine grant R21AT003735 (CLS) and the National Institutes of Health/National Cancer Institute grant CA90997 (JSW).

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Department of Pathology, Texas Tech University Health Sciences Center, BB 198, 3601 4th Street, Lubbock, TX, 79430-9097, USA

    C.-L. Shen, C. Samathanam, R. Y. Dagda, M.-C. Chyu & D. M. Dunn

  2. Laura W. Bush Institute for Women’s Health, Texas Tech University Health Sciences Center, Lubbock, TX, USA

    C.-L. Shen

  3. Applied Bench Core Laboratory, Winthrop-University Hospital, Mineola, NY, USA

    J. K. Yeh

  4. USDA ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, USA

    J. J. Cao

  5. Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA

    B. J. Stoecker

  6. Department of Mechanical Engineering, Texas Tech University, Lubbock, TX, USA

    M.-C. Chyu

  7. Graduate Healthcare Engineering Option, Texas Tech University, Lubbock, TX, USA

    M.-C. Chyu

  8. Department of Environmental Health Science, University of Georgia, Athens, Athens, GA, USA

    J.-S. Wang

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  1. C.-L. Shen
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  2. J. K. Yeh
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  3. C. Samathanam
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  9. J.-S. Wang
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Correspondence to C.-L. Shen.

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Shen, CL., Yeh, J.K., Samathanam, C. et al. Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation. Osteoporos Int 22, 327–337 (2011). https://doi.org/10.1007/s00198-010-1209-2

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  • DOI: https://doi.org/10.1007/s00198-010-1209-2

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