Abstract
The objective of this study was to examine the distribution of rDNA loci in the genus Glycine Willd. by fluorescent in situ hybridization (FISH) using the internal transcribed spacer (ITS) region of nuclear ribosomal DNA as a probe. The hybridized rDNA probe produced two distinct yellow signals on reddish chromosomes representing two NORs in 16 diploid (2n=40) species. Aneudiploid (2n=38) and aneutetraploid (2n=78) Glycine tomentella Hayata also exhibited two rDNA sites. However, the probe hybridized with four chromosomes as evidenced by four signals in two diploid species (Glycine curvata Tind. and Glycine cyrtoloba Tind.) and tetraploid (2n=80) G. tabacina (Labill.) Benth. and G. tomentella. Synthesized amphiploids (2n=80) of Glycine canescens F. J. Herm. (2n=40) and the 40-chromosome G. tomentella also showed four signals. This study demonstrates that the distribution of the rDNA gene in the 16 Glycine species studied is highly conserved and that silence of the rDNA locus may be attributed to amphiplasty during diploidization and speciation.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 10 October 2000 / Accepted: 6 December 2000
Rights and permissions
About this article
Cite this article
Singh, R., Kim, H. & Hymowitz, T. Distribution of rDNA loci in the genus Glycine Willd.. Theor Appl Genet 103, 212–218 (2001). https://doi.org/10.1007/s001220100591
Issue Date:
DOI: https://doi.org/10.1007/s001220100591