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Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea

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Abstract

Publicly available genomic tools help researchers integrate information and make new discoveries. In this paper, we describe the development of immortal mapping populations of rapid cycling, self-compatible lines, molecular markers, and linkage maps for Brassica rapa and B. oleracea and make the data and germplasm available to the Brassica research community. The B. rapa population consists of 160 recombinant inbred (RI) lines derived from the cross of highly inbred lines of rapid cycling and yellow sarson B. rapa. The B. oleracea population consists of 155 double haploid (DH) lines derived from an F1 cross between two DH lines, rapid cycling and broccoli. A total of 120 RFLP probes, 146 SSR markers, and one phenotypic trait (flower color) were used to construct genetic linkage maps for both species. The B. rapa map consists of 224 molecular markers distributed along 10 linkage groups (A1–A10) with a total distance of 1125.3 cM and a marker density of 5.7 cM/marker. The B. oleracea genetic map consists of 279 molecular markers and one phenotypic marker distributed along nine linkage groups (C1–C9) with a total distance of 891.4 cM and a marker density of 3.2 cM/marker. A syntenic analysis with Arabidopsis thaliana identified collinear genomic blocks that are in agreement with previous studies, reinforcing the idea of conserved chromosomal regions across the Brassicaceae.

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Acknowledgments

We would like to thank Dr. Maria Laura Federico for her critical reading of the manuscript and Dr. Carlos Quiros and two anonymous referees for their helpful and constructive comments which have improved the contents of this manuscript. The authors would also like to thank Robert Vogelzang and Amy Van der Voort for their technical support.

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Authors and Affiliations

  1. Agri aquaculture Nutritional Genomic Center (CGNA), Plant Biotechnology Unit (UBP), INIA-Carillanca, P.O. Box 58-D, Temuco, Chile

    Federico Luis Iniguez-Luy

  2. Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI, 53706, USA

    Federico Luis Iniguez-Luy

  3. Department of Plant Agriculture, University of Guelph, Crop Science Building, Guelph, ON, N1G 2W1, Canada

    Lewis Lukens

  4. USDA-ARS, 2700 Savannah Highway, Charleston, SC, 29414, USA

    Mark W. Farnham

  5. Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI, 53706, USA

    Richard M. Amasino

  6. Seminis Vegetables Seeds, 36437 State Highway 16, Woodland, CA, 95695, USA

    Thomas C. Osborn

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  1. Federico Luis Iniguez-Luy
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  2. Lewis Lukens
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  3. Mark W. Farnham
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  4. Richard M. Amasino
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  5. Thomas C. Osborn
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Correspondence to Federico Luis Iniguez-Luy.

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Communicated by C. Quiros.

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Iniguez-Luy, F.L., Lukens, L., Farnham, M.W. et al. Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea . Theor Appl Genet 120, 31–43 (2009). https://doi.org/10.1007/s00122-009-1157-4

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