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Ornamental plants and thrips populations associated with tomato spotted wilt virus in Greece

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Abstract

A survey was conducted in order to record the ornamental plants that are hosts of tomato spotted wilt virus (TSWV) and impatiens necrotic spot virus (INSV) in Greece. Polyclonal antibodies prepared against the N protein of a Greek isolate of TSWV fromGerbera jamesonii (GR-34) were used. Leaf samples were taken from plants showing typical symptoms of tospovirus infection such as chlorotic and necrotic rings on the leaves and malformation and necrosis of the flowers. The samples were tested by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using polyclonal antibodies to the N proteins of TSWV and INSV (NL-07). ELIS A-positive samples were mechanically transmitted to plants ofPetunia hybrida, Nicotiana rustica andN. benthamiana to confirm infection. Although none of the samples was found infected with INSV, TSWV presence was recorded in 42 botanical species that belong to 40 genera in 27 families. Among them the speciesBeloperone guttata, Coleus barbatus, Impatiens petersiana andLilium auratum are reported for the first time as hosts of TSWV, whereasBegonia sp.,Catharanthus roseus Celosia cristata, Dianthus chinensis, Fuchsia hybrida andStephanotis floribunda are found as new hosts of the virus in Greece. Thrips collected from TSWV-infected plants were in most cases identified asFrankliniella occidentalis, except from plants ofDendranthema sp. andDianthus caryophyllus whereThrips tabaci individuals were also identified. Different percentages of transmitters were noticed when the thrips populations collected from TSWV-infected ornamental hosts were tested for transmission of TSWV.

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References

  1. Avrameas, S. (1969) Coupling of enzymes to proteins with glutaraldehyde.Immunochemistry 6:43–52.

    Article  PubMed  CAS  Google Scholar 

  2. Chatzivassiliou, E.K., Katis, N.I. and Peters, D. (1998) Transmission of tomato spotted wilt virus (TSWV) byThrips tabaci grown on tobacco or non tobacco crops.Proc. 4th Int. Symp. on Tospovirus and Thrips in Floral and Vegetable Crops (Wageningen, the Netherlands), pp. 59–62.

  3. Chatzivassiliou, E., Livieratos, J., Avgelis, A., Katis, N. and Lykouressis, D. (1996) Occurrence of tomato spotted wilt virus in vegetable and ornamental crops in Greece.Acta Hortic. 431:49–54.

    Google Scholar 

  4. Chatzivassiliou, E.K., Nagata, T., Katis, N.I. and Peters, D. (1999) The transmission of tomato spotted wilt tospovirus (TSWV) byThrips tabaci Lind. (Thysanoptera: Thripidae) populations originating from leek.Plant Pathol. 48:700–706.

    Article  Google Scholar 

  5. Clark, M.E and Adams, A.N. (1977) Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses.J. Gen. Virol. 34:475–483.

    Article  PubMed  CAS  Google Scholar 

  6. Corts, I., Livieratos, I.C., Dercks, A., Peters, D. and Kormelink, R. (1998) Molecular and serological characterisation of iris yellow spot virus, anew distinct tospovirus species.Phytopathology 88:1276–1282.

    Article  Google Scholar 

  7. De Avila, A.C., De Haan, P., Kitajima, E.W., Kormelink, R., Resende, R. de O., Goldbach, R.W. and Peters, D. (1992) Characterisation of a distinct isolate of tomato spotted wilt virus (TSWV) fromImpatiens sp. in the Netherlands.J. Phytopathol. 134:133–151.

    Article  Google Scholar 

  8. Goldbach, R.W. and Peters, D. (1994) Possible causes of the emergence of Tospovirus diseases.Sem. Virol. 5:113–120.

    Article  Google Scholar 

  9. Jenser, G. (1982) Tripszek—Thysanoptera.in: Magyarorszag Allatvilaga [Fauna Hungariae]. Vol. 13. Akademiai Kiado, Budapest, Hungary.

    Google Scholar 

  10. Katis, N. and Avgelis, A. (1991) [Tomato spotted wilt virus: New threat for tomato and pepper crops in Northern Greece.]Abstr. 15th Scientific Meeting of the Greek Society of Horticulture (Thessaloniki, Greece), p. 69 (in Greek).

  11. Lindorf, M.B. (1931) Further studies of the transmission of the pineapple yellow spot virus byThrips tabaci.Phytopathology 21:999.

    Google Scholar 

  12. Lindorf, M.B. (1932) Transmission of the pineapple yellow-spot virus byThrips tabaci.Phytopathology 22:301–324.

    Google Scholar 

  13. Livieratos, I.C., Lykouressis, D. and Katis, N.I. (1994) [Studies on the genusTospovirus in Northern Greece.]Abstr. 7th. Panhellenic Phytopathological Congr. (Athens, Greece), p. 41 (in Greek).

  14. Matteoni, J.A. and Allen, W.R. (1989) Symptomatology of tomato spotted wilt virus infection in florist’s chrysanthemum.Can. J. Plant Pathol. 11:373–380.

    Google Scholar 

  15. Murphy, F.A., Fauquet, G.M., Bishop, D.H.L., Ghabrial, S.A., Jarvis, A.W., Martelli, G.P., Mayo, M.A. and Summers, M.D. (1995) Virus Taxonomy. Sixth Rep. Int. Committee on Taxonomy of Viruses.Arch. Virol. Suppl. 10:1–586.

    Google Scholar 

  16. Palmer, J.M., Mound, L.A. and Heaume, G.J. (1992)in: Betts, C.R. [Ed.] HIE Guides to Insects of Importance to Man: Thysanoptera. CAB International, Wallingford, UK.

    Google Scholar 

  17. Peters, D. (1998) An updated list of plant species susceptible to tospoviruses. pp. 107–110.in: Peters, D. and Goldbach, R.W. [Eds.] Recent Progress in Tospovirus and Thrips Research. Wageningen Agricultural University, Wageningen, the Netherlands.

    Google Scholar 

  18. Priesner, H. (1964) Ordnung Thysanoptera. Akademie-Verlag, Berlin, Germany.

    Google Scholar 

  19. Sakimura, K. (1962) The present status of thrips-borne viruses. pp. 33–40.in: Maramorosch, K. [Ed.] Biological Transmission of Disease Agents. Academic Press, New York, NY.

    Google Scholar 

  20. Selman, I.W., Brierley, G.F., Pegg, G.F. and Hill, T.A. (1961) Changes in the free amino acids and amides in tomato plants inoculated with tomato spotted wilt virus.Ann. Appl. Biol. 49:601–615.

    Article  CAS  Google Scholar 

  21. Strassen, R. zur (1986)Frankliniella occidentalis (Pergande, 1895) ein nordamerikanischer Fransenflugler (Thysanoptera) als neuer Bewohnereuropaischer Gewachshauser. Nachrichtenbl.Dtsch. Pflanzenschutzdienst. (Stuttgart) 38(6):86–88.

    Google Scholar 

  22. Tsakiridis, J.P.and Gooding, G.V. Jr. (1972) Tomato spotted wilt virus in Greece.Phytopathol. Mediterr. 11:42–47.

    Google Scholar 

  23. Ullman, D.E., Cho, J.J., Mau, R.F.L., Hunter, W.B., Westcott, D.M. and Custer, D.M. (1992) Thrips- tomato spotted wilt virus interaction: morphological, behavioral and cellular components influencing thrips transmission.Adv. Dis. Vector Res. 9:195–240.

    Google Scholar 

  24. Vaira, A.M., Roggero, P., Luisoni, E., Masenga, V., Milne, R.G. and Lisa, V. (1993) Characterization of two tospoviruses in Italy: tomato spotted wilt and impatiens necrotic spot.Plant Pathol. 42:530–542.

    Article  CAS  Google Scholar 

  25. Van deWetering, F., Goldbach, R. and Peters, D. (1996) Tomato spotted wilt virus ingestion by first instar larvae ofFrankliniella occidentalis is a prerequisite for transmission.Phytopathology 86:900–905.

    Article  Google Scholar 

  26. Volvas, C. and Avgelis, A. (1994) Infezione da TSWV Tospovirus su poinodoro e peperone nell isola di Creta.Inf. Fitopatol. 1:55–56.

    Google Scholar 

  27. Wijkamp, I. and Peters, D. (1993) Determination of the median latent period of two tospoviruses inFrankliniella occidentalis, using a novel leaf disk assay.Phytopathology 82:986–991.

    Article  Google Scholar 

  28. Yudin, L.S., Tabashnik, B.E., Cho, J.J. and Mitchell, W.C. (1988) Colonization of weeds and lettuce by thrips (Thysanoptera: Thripidae).Environ. Entomol. 17:522–526.

    Google Scholar 

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Author notes

  1. I. Livieratos

    Present address: Dept. of Biology, Imperial College of Science, Technology & Medicine, SW7 2A2, London, UK

Authors and Affiliations

  1. Plant Pathology Laboratory, Faculty of Agriculture, Aristotle University of Thessaloniki, 540 06, Thessaloniki, Greece

    E. K. Chatzivassiliou, I. Livieratos & N. I. Katis

  2. Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary

    G. Jenser

Authors
  1. E. K. Chatzivassiliou
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  2. I. Livieratos
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  3. G. Jenser
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  4. N. I. Katis
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Correspondence to E. K. Chatzivassiliou.

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Chatzivassiliou, E.K., Livieratos, I., Jenser, G. et al. Ornamental plants and thrips populations associated with tomato spotted wilt virus in Greece. Phytoparasitica 28, 257–264 (2000). https://doi.org/10.1007/BF02981804

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  • DOI: https://doi.org/10.1007/BF02981804

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