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Lime pretreatment of switchgrass

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Abstract

Lime (calcium hydroxide) was used as a pretreatment agent to enhance the enzymatic digestibility of switchgrass. After studying many conditions, the recommended pretreatment conditions are: time = 2 h, temperature = 100°C and 120°C, lime loading = 0.1 g Ca(OH)2/g dry biomass, water loading = 9 mL/g dry biomass. Studies on the effect of particle size indicate that there was little benefit of grinding below 20 mesh; even coarse particles (4–10 mesh) digested well. Using the recommended pretreatment conditions, the 3-d reducing sugar yield was five times that of untreated switchgrass, the 3-d total sugar (glucose + xylose) yield was seven times, the 3-d glucose yield was five times, and the 3-d xylose yield was 21 times. A material balance study showed that little glucan (approx 10%) was solubilized as a result of the lime pretreatment, whereas about 26% of xylan and 29% of lignin became solubilized.

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References

  1. Klyosov, A. A. (1986),Appl. Biochem. Biotechnol.12, 249–300.

    Article  Google Scholar 

  2. Fan, L. T., Lee, Y.-H., and Gharpuray, M. M. (1982),Adv. Biochem. Eng. 23, 157–187.

    CAS  Google Scholar 

  3. Kong, R., Engler, C. R. and Soltes, E. J. (1992),Appl. Biochem. Biotechnol. 34, 23–35.

    Article  Google Scholar 

  4. Lin, K. W., Ladisch, M. R., Schaefer, D. M., Noller, C. H., Lechtenberg, V., and Tsao, G. T. (1981),AIChE Symposium Series 207, vol.77, 102–106.

    CAS  Google Scholar 

  5. Fan, L. T., Gharpuray, M. M., and Lee, Y.-H., (1987), inCellulose Hydrolysis, Springer-Verlag, Berlin, pp. 52–69.

    Google Scholar 

  6. Chang, M. M., Chou, T. Y. C., and Tsao, G. T. (1982),Adv. Biochem. Eng. 20, 15–42.

    Google Scholar 

  7. Weil, J., Westgate, P., Kohlmann, K., and Ladisch, M. R. (1994),Enzyme Microb. Technol. 16, 1002–1004.

    Article  CAS  Google Scholar 

  8. Jackson, M. G. (1977),Anim. Feed Sci. Technol. 2, 105–130.

    Article  Google Scholar 

  9. Anderson, D. C. and Ralston, A. T. (1973),J. Anim. Sci. 37, no. 1, 148–152.

    CAS  Google Scholar 

  10. Lesoing, G., Klopfenstein, T., Rush, I., and Ward, J. (1981),J. Anim. Sci. 51, no. 2, 263–269.

    Google Scholar 

  11. Clunningham, R. L. and Carr, M. E. (1984),Biotechnol. Bioeng. Symp. 14, 95–103.

    Google Scholar 

  12. Peters, M. S. and Timmerhaus, K. D. (1991), inPlant Design and Economics for Chemical Engineers, McGraw-Hill, New York, NY, p. 816.

    Google Scholar 

  13. Holtzapple, M. T., Jun, J.-H., Ashok, G., Patibandla, S. L., and Dale, B. E. (1991),Appl. Biochem. Biotechnol. 28, 59–74.

    Google Scholar 

  14. Holtzapple, M. T., Lundeen, J. E., Sturgis, R., Lewis, J. E., and Dale, B. E. (1992),Appl. Biochem. Biotechnol. 34, 5–21.

    Google Scholar 

  15. Nagwani, M. (1992), M.S. thesis, Texas A&M University, College Station, Texas.

    Google Scholar 

  16. Abou-Raya, A. K., Abou-Hussein, E. R. M., Ghoneim, A., Raafat, M. A., and Mohamed, A. A. (1964),J. Anim. Prod. U. A. R. 4, no. 1, 55–65.

    Google Scholar 

  17. Waller, J. C. and Klopfenstein, T. (1975),J. Anim. Sci. 41, no. 1, 242–425 (abstract).

    Google Scholar 

  18. Owen, E. and Nwadukwe, B. S. (1980),Anim. Prod. 30, 489 (abstract).

    Google Scholar 

  19. Djajanegara, A., Molina, B. T., and Doyle, P. T. (1984),Anim. Feed Sci. Technol. 12, 141–150.

    Article  Google Scholar 

  20. Oliveros, B. A., Britton, R. A., and Klopfenstein, T. J. (1993),Anim. Feed Sci. Technol. 44, 59–72.

    Article  CAS  Google Scholar 

  21. Gharib, F. H., Meiske, J. C., Goodrich, R. D., and Serafy, A. M. (1975),J. Anim. Sci. 40, no. 4, 734–742.

    CAS  Google Scholar 

  22. Rounds, W., Klopfenstein, T., Waller, J., and Messersmith, T. (1976),J. Anim. Sci. 43, no. 2, 478–482.

    CAS  Google Scholar 

  23. Wilkinson, J. M. and Santillana, R. G. (1978),Anim. Feed Sci. Technol. 3, 117–132.

    Article  CAS  Google Scholar 

  24. Paterson, J. A., Stock, A. R., and Klopfenstein, T. J. (1980), inCalcium Hydroxide Treatment of Crop Residues, Nebraska Beef Cattle Rep., EC 80–218, 21.

  25. Ibrahim, M. N. M. and Pearce, G. R. (1983),Agricultural Wastes 5, 135–156.

    Article  CAS  Google Scholar 

  26. Playrte, M. J. (1984),Biotechnol. Bioeng. 26, 42–433.

    Google Scholar 

  27. Winugroho, M., Ibrahim, M. N. M., and Pearce, G. R. (1984),Agricultural Wastes 9, 87–89.

    Article  CAS  Google Scholar 

  28. Owen, E., Klopfenstein, T., and Urio, N. A. (1984), inStraw and Other Fibrous By-products as Feed, Sundstøl, F. and Owen, E., eds., Elsevier Science Publishers B. V., Amsterdam, Netherlands, pp. 248–275.

    Google Scholar 

  29. Felix, A., Hill, R. A., and Diarra, B. (1990),Anim. Prod. 51, 47–61.

    Google Scholar 

  30. Mohlenbrock, R. H. (1973), inThe Illustrated Flora of Illinois: Grasses Panicum to Danthonia, Feffer & Simons, Inc., London and Armsterdam, pp. 67, 68.

    Google Scholar 

  31. Gould, F. W. (1968), inGrass Systematics, McGraw-Hill, New York, p. 214.

    Google Scholar 

  32. Chemical Analysis & Testing Standard Procedure, National Renewable Energy Laboratory.

  33. Miller, G. L. (1959),Anal. Chem. 31, 426–428.

    Article  CAS  Google Scholar 

  34. Holtzapple, M. T., Ripley, E. P., and Nikolaou, M. (1994),Biotechnol. Bioeng. 44, 1122–1131.

    Article  CAS  Google Scholar 

  35. Burr, B., Chang, V. S., Holtzapple, M. T., and Davidson, R. (1996), inDevelopment of Alternative Pretreatment and Biomass Fractionation Processes: Lime Pretreatment, Final Report, Part II, Subcontract XAW-3-11181-03, National Renewable Energy Laboratory.

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Chang, V.S., Burr, B. & Holtzapple, M.T. Lime pretreatment of switchgrass. Appl Biochem Biotechnol 63, 3–19 (1997). https://doi.org/10.1007/BF02920408

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