Abstract
The diffusion coefficients of lysozyme and alpha-amylase were measured in the various polyethylene glycol (PEG) solutions. Obtained diffusion coefficients were studied with the viscosity coefficient of the solution. It was found that the diffusion process of the protein was suppressed with a factor of vγ, where ν is a relative viscosity coefficient of the PEG solution. The value of γ is −0.64 at PEG1500 for both proteins. The value increased to −0.48 at PEG8000 for lysozyme, while decreased to −0.72 for alpha-amylase. The equation of an approximate diffusion coefficient at certain PEG molecular weight and concentration was roughly obtained.
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Otalora F., Novella M.L., Gavira J. A., Thomas B. R., Garcia-Ruiz J. M.: Experimental Evidence for the Stability of the Depletion Zone Around a Growing Protein Crystal under Microgravity. Acta Cryst., 2001, D57, 412–417
McPherson A.: Crystallization of Biological Macromolecules. Cold Spring Harbor Lab. Press, 1999
Tanaka H., Inaka K., Sugiyama S., Takahashi S., Sano S., Sato M., Yoshitomi S.: Numerical Analysis of the Depletion Zone Formation Around a Growing Protein Crystal. Ann. N.Y. Acad. Sci., 2004, 1027, 10–19
Ninomiya K., Yamamoto T., Oheda T., Sato K., Sazaki G., Matsuura Y.: Morphology and Solubility of Multiple Crystal Forms of Taka-amylase A. J. Crystal Growth, 222, 311–316, 2001
Nakamura H., Fukuyama S., Yoshizaki I., Yoda S.: Diffusion Coefficient Measurement for Proteins by Interferometoric Method. Thermophysical Properties, 22, 259–261, 2001
Yoshizaki I., Nakamura H., Fukuyama S., Yoda S., Komatsu H.: Scientific Approach on the Optimization of Protein Crystallization Condition for Microgravity Experiments. Ann. N.Y. Acad. Sci., 2004, 1027, 28–47.
Garcia-Ruiz J.M., Moreno A.: Investigation on Protein Crystal Growth by the Gel Acupuncture Method. Acta Cryst. 1994, D50, 484–490
Tanaka H., Inaka K., Sugiyama S., Takahashi S., Sano S., Sato M., Yoshitomi S.: A Simplified Counter Diffusion Method Combined with a 1D simulation Program for Optimizing Crystallization Conditions. J. Synchrotron Rad. 2004, 11, 45–48
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Tanaka, H., Takahashi, S., Yamanaka, M. et al. Diffusion coefficient of the protein in various crystallization solutions: The key to growing high-quality crystals in space. Microgravity Sci. Technol 18, 91–94 (2006). https://doi.org/10.1007/BF02870387
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DOI: https://doi.org/10.1007/BF02870387