Abstract
During peroxidation, phosphatidyl ethanolamine and phosphatidyl serine formed fluorescent chromophores with maximum emission at 435 nm and maximum excitation at 365 nm. The development of fluorescence was related to formation of thiobarbituric acid reactive substance during lipid peroxidation. This relationship was studied by reacting dipalmityl phosphatidyl ethanolamine with the oxidation products of the methyl esters of arachidonic, linolenic and linoleic fatty acids. Reaction parameters affecting the development of lipid-extractable fluorescent chromophores are: the production of peroxidation products, especially malonaldehyde, from autoxidation of polyunsaturated fatty acids; the length of time these products react; and the availability of reactive amino groups on the phospholipids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Packer, L., D.W. Deamer and R.L. Heath, Advan. Gerontol. Res. 2:77 (1967).
Tappel, A.L., “N.Y. Academy of Sciences Conference on Vitamin E, 1971,” Ann. N.Y. Acad. Sci. 203:12 (1972).
Fleischer, S., and G. Rouser, JAOCS 43:588 (1965).
Lillard, D., and E.A. Day, Ibid. 41:549 (1965).
Dahle, L.K., E.G. Hill and R.T. Holman, Arch. Biochem. Biophys. 98:253 (1962).
Tarladgis, B.G., and B.M. Watts, JAOCS 37:403 (1960).
Crawford, D.L., T.C. Yu and R.O. Sinnhuber, Agr. Food Chem. 14:182 (1966).
Crawford, D.L., T.C. Yu and R.O. Sinnhuber, J. Food Sci. 32:332 (1967).
Chio, K.S., and A.L. Tappel, Biochemistry 8:2821 (1969).
Chio, K.S., and A.L. Tappel, Ibid. 8:2827 (1969).
Dillard, C.J., and A.L. Tappel, Lipids 6:715 (1971).
Kwon, T.W., and B.M. Watts, J. Food Sci. 28:627 (1963).
Derivician, D.G., Progr. Biophys. Mol. Biol. 14:263 (1964).
Bangham, A.D., Ibid. 18:29 (1968).
Chio, K.S., U. Reiss, B. Fletcher and A.L. Tappel, Science 166:1535 (1969).
Keeney, M., in “Lipids and Their Oxidation,” Edited by H.W. Schultz, E.A. Day and R.O. Sinnhuber, Avi Publishing Co., Inc., Connecticut, 1962, p. 79.
Kwon, T.W., and H.S. Olcott, J. Food Sci. 31:552 (1966).
Kwon, T.W., and B.M. Watts, Ibid. 29:294 (1964).
Corliss, G.A., and L.R. Dugan, Jr., Lipids 5:846 (1970).
Menzel, D.B., Ibid. 2:83 (1967).
Sawicki, E., T.W. Stanley and H. Johnson, Anal. Chem. 35:199 (1963).
Tappel, A.L., in “Pathological Aspects of Cell Membranes,” Vol. 1, Edited by B.F. Trump and A. Arstila, Academic Press, New York, In press, 1973.
Bidlack, W.R., Ph.D. thesis, University of California, Davis, 1972.
Author information
Authors and Affiliations
About this article
Cite this article
Bidlack, W.R., Tappel, A.L. Fluorescent products of phospholipids during lipid peroxidation. Lipids 8, 203–207 (1973). https://doi.org/10.1007/BF02544636
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02544636