Abstract
The role of acyclic eicosanoids in the modulation of the plasticity of somatic cholinoreceptors by 15(S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (15-HETE) was investigated in identified RPa3 and LPa3 neurons ofHelix lucorum using the two-electrode voltage clamping technique. It was demonstrated that the inhibitor of lipoxygenases, nordihydroguaiaretic acid (2–20 μM), completely blocks the short- and long-latency effects of 15-HETE (2–20 μM) on the depth of damping of the inward current induced by rhythmic applications of acetylcholine (ACh) to the soma. It is concluded that the short-latency effect of 15-HETE on the plasticity of cholinoreceptors is determined by its inhibition mainly of 5-lipoxygenase, which leads to a reduction in the level of acyclic eicosanoids that are formed under the influence of this enzyme. The potentiation of the effects of the acyclic eicosanoids, whose synthesis is resumed in the second phase, probably underlies the direct longlatency modulatory effect of 15-HETE.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
B. I. Kotlyar and A. S. Pivovarov, “The molecular mechanism of the plasticity of the neuron during learning: the role of second messengers,”Zh. Vyssh. Nerv. Deyat.,39, No. 2, 195 (1989).
A. S. Pivovarov and E. I. Drozdova, “The identification of cholinoreceptors in the soma of RPa3 and LPa3 neurons of neurons of the common snail”Neirofiziologiya,24, No. 1, 77 (1992).
A. S. Pivovarov, E. I. Drozdova, Yu. Yu. Belosludtsev, et al., “Short- and long-latency effects of 15-hydroxyeicotetraenoic [sic] acid on the extinction of the acetylcholine-induced inward current of neurons of the common snail,”Byull. Éksp. Biol. Med.,112, No. 7, 3 (1991).
A. S. Pivovarov, E. I. Drozdova, and B. I. Kotlyar, “Arachidonic acid and its acyclic derivatives regulate the shortterm plasticity of cholinoreceptors of neurons of the common snail,”Zh. Vyssh. Nerv. Deyat.,41, No. 4, 796 (1991).
A. S. Pivovarov, E. I. Drozdova, D. A. Zabolotskii, and G. I. Myagkova, “Inhibitors of the lipoxygenases, the eicosapolyynoic acids, attenuate the short-term plasticity of the cholinoreceptors of neurons of the common snail,”Zh. Vyssh. Nerv. Deyat.,41, No. 6, 1215 (1991).
J. Axelrod, R. M. Burch, and C. L. Jelsema, “Receptor-mediated activation of phospholipase A2 via GTP-binding proteins: arachidonic acid and its metabolites as second messengers,”Trends Neurosci.,11, 117 (1988).
S. Bevan and J. N. Wood, “Arachidonic acid metabolites as second messengers,”Nature,328, 20 (1987).
C. Galli and A. Petroni, “Eicosanoids and the central nervous system,”Uppsala J. Med. Sci., Suppl. 48, 133 (1990).
A. S. Gukovskaya, H. Arias Pulido, V. V. Petrunyaka, et al., “Lipoxygenase inhibitors suppress intracellular calcium rise induced by ionomycin in rat thymocytes,”Cell Calcium,11, 539 (1990).
W. C. Hope, A. F. Welton, C. Fiedler-Nagy, et al., “In vitro inhibition of the biosynthesis of slow reacting substance of anaphylaxis (SRS-A) and lipoxygenase activity by quercetin,”Biochem. Pharmacol.,32, 367 (1983).
J. C. Louis, P. Basset, M. O. Revei, et al., “Opposite effects of arachidonic acid and of its hydroperoxides on brain soluble guanylate cyclase activity,”Neurochem. Int.,18, 131 (1991).
D. Piomelli and P. Greengard, “Lipoxygenase metabolites of arachidonic acid in neuronal transmembrane signalling,”Trends Pharmacol. Sci.,11, 367 (1990).
J. P. Robinson and D. A. Kendall, “No role for phospholipase A2 and protein kinase C in the potentiation by a-receptors of b-adrenergic-mediated cyclic AMP formation in rat brain,”J. Neurochem.,51, 542 (1989).
I. Rustenbeck and S. Lenzen, “Effects of lysophosphatidylcholine and arachidonic acid on the regulation of intracellular Ca2+ transport,”Naunyn-Schmied. Arch.,339, 37 (1989).
T. Shimizu and L. S. Wolfe, “Arachidonic acid cascade signal transduction,”J. Neurochem.,55, 1 (1990).
J. Strosznajder and M. Samochocki, “Carbachol-stimulated release of arachidonic acid and eicosanoids from brain cortex synaptoneurosomes of adult and aged rats,”Adv. Exptl. Med. Biol.,318, 251 (1992).
P. Vacher, J. McKenzie, and B. Dufy, “Arachidonic acid enhances cytosolic free Ca2+ concentration in CH3 pituitary cells,”Arch. Int. Physiol. Biochim.,98, 253 (1990).
J. Y. Vanderhoek, R. M. Bryant, and J. M. Bailey, “15-Hydroxy-5,8,11,13-eicosatetraenoic acid, a potent and selective inhibitor of platelet lipoxygenase,”J. Biol. Chem.,255, 5996 (1980).
J. Y. Vanderhoek, R. M. Bryant, and J. M. Bailey, “Inhibition of leukotriene biosynthesis by the leukocyte product 15-hydroxy-5,8,11,13-eicosatetraenoic acid,”J. Biol. Chem.,255, 10064 (1980).
Additional information
Department of Higher Nervous Activity, M. V. Lomonosov Moscow State University. Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti imeni I. P. Pavlova, Vol. 44, No. 4-5, pp. 806–811, July–October, 1994.
Rights and permissions
About this article
Cite this article
Pivovarov, A.S., Egido-Villareal, W. The influence of an inhibitor of lipoxygenases on the modulation of the plasticity of cholinoreceptors by 15-hete. Neurosci Behav Physiol 25, 483–487 (1995). https://doi.org/10.1007/BF02359276
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02359276