Abstract
Expression of endothelial adhesion molecules is a key factor in localizing inflammatory processes. We have evaluated the time-course expression of E-selectin, ICAM-1, and VCAM-1 in rectal mucosae from coeliac patients (CD) with T-lymphocyte sensitization to wheat (gliadin) protein. Ten treated, eight untreated CD patients and five disease controls underwent a dynamic 4 h rectal gluten challenge. Seven treated CD patients had a 24 h challenge to evaluate the time-course changes. Coeliac tissue significantly increased VCAM-1 expression [p<0.001 (untreated and treated CD)] and E-selectin [p=0.01 (untreated CD only)]-expression compared to controls but not ICAM-1. VCAM-1 continued to increase up to 24 h, while E-selectin decreased after 4 h. These findings relate to an 8 h rise in CD3 (+) lymphocytes [p<0.05 (treated)] and a 4 h rise in neutrophils (p<0.005). This approach permits a novel study of the inflammatory response to a known immunogen in human patients.
Similar content being viewed by others
References
M. N. Marsh,Gluten, Major Histocompatibility Complex, and the Small Intestine. Gastroenterology102, 330–354 (1992).
D. E. Loft, M. N. Marsh, G. I. Sandle, P. T. Crowe, V. Garner, D. Gordon and R. Baker,Studies of Intestinal Lymphoid Tissue. XII. Epithelial Lymphocyte and Mucosal Responses to Rectal Gluten Challenge in Celiac Sprue. Gastroenterology97, 29–37 (1989).
T. A. Springer,Adhesion receptors of the immune system. Nature346, 425–434 (1990).
Y. Shimuzu, G. A. Van Seventer, K. J. Horgan, S. Shaw,Roles of adhesion molecules in T-cell recognition: Fundamental similarities between four integrins on resting human T cells (LFA-1, VLA-4, VLA-5, VLA-6) in expression, binding, and costimulation. Immunological Reviews114, 109–137 (1990).
C. J. Smart, A. Calabrese, D. J. Oakes, P. D. Howdle and L. K. Trejdosiewicz,Expression of the LFA-1 β2 integrin (CD11a/CD18) and ICAM-1 (CD54) in normal and coeliac small bowel mucosa. Scandinavian Journal of Immunology34, 299–305 (1991).
J. Norton, J. P. Sloane, N. Al-Saffar and D. O. Haskard,Expression of adhesion molecules in human intestinal graftversus-host disease. Clinical Experimental Immunology85, 109–113 (1991).
J. Spencer, P. G. Isaacson, T. T. MacDonald, A. J. Thomas and J. A. Walker-Smith,Gamma/delta T cells and the diagnosis of coeliac disease. Clinical Experimental Immunology85, 109–113 (1991).
R. P. Sturgess, J. C. Macartney, M. W. Makgoba, C. H. Hung, D. O. Haskard and P. J. Ciclitira,Differential upregulation of intercellular adhesion molecule-1 in coeliac disease. Clinical Experimental Immunology82, 489–492 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ensari, A., Ager, A., Marsh, M.N. et al. Observations of the time-course of the inflammatory response of rectal mucosa to gliadin challenge in gluten-sensitive subjects. Agents and Actions 38 (Suppl 2), C47–C50 (1993). https://doi.org/10.1007/BF01991133
Issue Date:
DOI: https://doi.org/10.1007/BF01991133