Summary
Intermittant long-term stimulation of fast rabbit muscles up to 28 days with a frequency pattern resembling that of a slow muscle (10 Imp/sec) led to a slowing of the time course of contraction already during the first week. There was an increase of tetanic tension as well. The observed rearrangement of activities of key enzymes of energy supplying metabolism was found to occur sequentially. Decreases of extramitochondrial enzymes of glycogenolysis (phosphorylase), glycolysis (triosephosphate dehydrogenase, lactate dehydrogenase) and energy rich phosphate transfer were found initially together with a decrease of mitochondrial glycerolphosphate dehydrogenase. The isozyme pattern of lactate dehydrogenase was changed. Large initial increases were found in enzymes involved in glucose phosphorylation (hexokinase) and fatty acid activation (palmitoyl-CoA synthetase). Later an increase of key enzymes of the citric acid cycle (citrate synthase) and fatty acid oxidation (3-hydroxyacyl-CoA dehydrogenase) as well as ketone body utilization (3-ketoacid-CoA transferase) could be shown.
Histochemical staining and comparative activity determination of succinate dehydrogenase in single fibres revealed that the mosaic like fibre composition of the fast muscle was transformed into a more uniform population resembling that of a predominantly slow muscle.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnard, J., Edgerton, V. P., Furukawa, T., Peter, J. B.: Amer. J. Physiol.220, 410 (1971).
Barnard, J., Edgerton, V. R., Peter, J. B.: J. appl. Physiol.28, 762 (1970).
Bass, A., Brdiczka, D., Eyer, P., Hofer, S., Pette, D.: Europ. J. Biochem.10, 198 (1969).
Brewer, G. J.: In: Introduction to isozyme techniques. New York-London: Academic Press 1970.
Bücher, Th., Luh, W., Pette, D.: In: Handbuch der physiologisch- und pathologisch-chemischen Analyse. Eds. Hoppe-Seyler/Thierfelder, Bd. VI/A, S. 292–339. Berlin-Heidelberg-New York: Springer 1964.
Bücher, Th., Pette, D.: In: Verhandlungen der Deutschen Gesellschaft für innere Medizin, 71. Kongreß, S. 104–124. München: J. F. Bergmann 1965.
Buller, A. J., Eccles, J. C., Eccles, R. M.: J. Physiol. (Lond.)150, 417 (1960).
Drahota, Z., Gutmann, E.: Physiol. bohemoslov.12, 339 (1963).
Dubowitz, V.: J. Physiol. (Lond.)193, 481 (1967).
Flavell, R. B., Woodward, D. O.: Europ. J. Biochem.17, 284 (1970).
Golisch, G., Pette, D., Pichlmaier, H.: Europ. J. Biochem.16, 110 (1970).
Guth, L., Watson, P. K., Brown, W. C.: Exp. Neurol.20, 52 (1968).
Gutmann, E., Hàjek, I.: Physiol. bohemoslov.20, 205 (1971).
Helmreich, E., Danforth, W. H., Karpatkin, S., Cori, C. F.: In: Control of energy metabolism, pp. 299–312. Eds. B. Chance, R. W. Estabrook, J. R. Williamson. New York-London: Academic Press 1965.
Holloszy, J. O., Narahara, H. T.: J. biol. Chem.240, 3493 (1965).
Holloszy, J. O., Oscai, L. B.: Arch. Biochem. Biophys.130, 653 (1969).
Holloszy, J. O., Oscai, L. B., Molé, P. A., Don, I. J. In: Muscle metabolism during exercise, pp. 51–61. Eds. B. Pernow, and B. Saltin. New York-London: Plenum Press 1971.
Issekutz, B., Spitzer, J.: Proc. Soc. exp. Biol. (N.Y.)105, 21 (1960).
Kornberg, A., Pricer, W. E., Jr.: J. biol. Chem.204, 329 (1953).
Kubista, V., Kubistová, J., Pette, D.: Europ. J. Biochem.18, 553 (1971).
Maitra, P. K., Lobo, Z.: J. biol. Chem.246, 475 (1971).
Nolte, J., Pette, D.: In: Current problems in clinical biochemistry. 3. Recent advances in quantitative histo- and cytochemistry, pp. 54–60. Eds. U. C. Dubach and U. Schmidt, Bern-Stuttgart-Vienna: H. Huber 1971.
Nolte, J., Pette, D.: J. Histochem. Cytochem.20, 567, 577 (1972).
Pette, D.: Naturwissenschaften52, 597 (1965).
Pette, D.: In: Muscle metabolism during exercise, pp. 33–50. Eds. B. Pernow and B. Saltin. New York-London: Plenum Press 1971.
Pette, D., Bücher, Th.: Hoppe-Seyler's Z. physiol. Chem.331, 180 (1963).
Pette, D., Staudte, H. W.: In: Limiting factors of physical performance. Ed. J. Keul. Stuttgart: Thieme 1973.
Prewitt, M. A., Salafsky, B.: Amer. J. Physiol.213, 295 (1967);218, 69 (1970).
Reynarfarje, B.: J. Lab. clin. Med.61, 138 (1963).
Ruderman, N. B., Houghton, C. R. S., Hems, R.: Biochem. J.124, 639 (1971).
Salmons, S., Vrbová, G.: J. Physiol. (Lond.)201, 535 (1969).
Staudte, H. W., Pette, D.: Comp. Biochem. Physiol.41B, 533 (1972).
Vrbová, G.: J. Physiol. (Lond.)169, 513 (1966).
Wieme, R. J.: In: Studies on agar-gel electrophoresis. Brussels: Arscia 1959.
Williamson, D. H., Bates, M. W., Page, M. A., Krebs, H. A.: Biochem. J.121, 41 (1970).
Author information
Authors and Affiliations
Additional information
This study was supported by grants from Medical Research Council, Deutsche Forschungsgemeinschaft and Deutscher Sportbund.
Rights and permissions
About this article
Cite this article
Pette, D., Smith, M.E., Staudte, H.W. et al. Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit muscles. Pflugers Arch. 338, 257–272 (1973). https://doi.org/10.1007/BF00587391
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00587391