Abstract
The antioxidative protection during the C3-CAM shift induced by water stress was investigated in the temperate succulent Sedum album L. The C3-CAM shift was characterized in terms of CO2 exchange, titratable acidity and phosphoenolpyruvate carboxylase activity. Well-watered plants displayed C3-like patterns of gas exchange and exhibited a mild day-night acid fluctuation indicating that those plants were performing CAM-cycling metabolism. Imposed drought highly stimulated CAM cycling, decreasing the net CO2 uptake during the day, eliminating net CO2 efflux at night and stimulating tissue acid fluctuations. As water deficit developed, chlorophyll fluorescence measurements showed a decrease in the Fv/Fm ratio, indicating that photoinhibition could follow after severe drought. Protection might be performed by the increased activity of enzymes involved in the destruction of free radicals and oxidants, but their response depended on the water status of the plant. Ascorbate peroxidase and superoxide dismutase activities increased in plants subjected to mild stress but declined during severe water stress. Catalase activity, however, was quite stable under mild water stress and was clearly inhibited under severe water stress. At this stage, glutathione reductase and monodehydroascorbate reductase seemed to be very important in the protection against oxidants, both increasing considerably their activities under severe water stress. Even if recycling has been shown to alleviate photoinhibition, our results clearly demonstrate that antioxidative enzymes play an important role in the protection of plants from oxidants during the C3-CAM shift induced by water stress.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams WW, Osmond CB (1988) Internal CO2 supply during photosynthesis of sun and shade grown CAM plants in relation to photoinhibition. Plant Physiol 86:117–123
Baisak R, Rana D, Acharya PB, Kar M (1994) Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol 35:489–495
Beauchamp C, Fridovich I (1974) Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. Anal Biochem 44:276–287
Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Bjorkman O, Powles SB (1984) Inhibition of photosynthetic reactions under water stress: interaction with light level. Planta 161:490–504
Bolhlar-Nordenkamp HR, Long SP, Baker NR, Oquist G, Schreiber U, Lechner EG (1989) Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation. Funct Ecol 3:497–515
Borland AM, Griffiths H (1990) The regulation of CAM and respiratory recycling by water supply and light regime in the C3-CAM intermediate Sedum telephium. Funct Ecol 4:33–39
Borland AM, Griffiths H (1992) Properties of phosphoenolpyruvate carboxylase and carbohydrate accumulation in the C3-CAM intermediate Sedum telephium L. grown under different light and watering regimes. J Exp Bot 43:353–361
Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Castillo FJ, Greppin H (1986) Balance between anionic and cationic extracellular peroxidase activities in S. album leaves after ozone exposure. Analysis by high-performance liquid chromatography. Physiol Plant 68:201–208
Castillo FJ, Greppin H (1988) Extracellular ascorbic acid and enzyme activities related to ascorbic acid metabolism in S. album L. leaves after ozone exposure. Environ Exp Bot 28:231–238
Chameides WL (1989) The chemistry of ozone deposition to plant leaves: role of ascorbic acid. Environ Sci Technol 23:595–599
Chen GX, Asada K (1992) Inactivation of ascorbate peroxidase by thiols require hydrogen peroxide. Plant Cell Physiol 33:117–123
Chowdhury SR, Choudhuri MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiol Plant 65:503–507
Demming-Adams B, Adams WW (1992) Photoprotection and other responses to high light stress. Annu Rev Plant Physiol Plant Mol Biol 43:599–626
Demming-Adams B, Adams WW, Winter K, Meyer A, Screiber U, Pereira JS, Kruger A, Czygan FC, Lange OL (1989) Photochemical efficiency of photosystem II, photon yield of O2 evolution, photosynthetic capacity and carotenoid composition during the midday depression of net CO2 uptake in Arbutus unedo growing in Portugal. Planta 177:377–387
Earnshaw MJ, Carver KA, Lee JA (1985) Changes in leaf water potential and CAM in Sempervivum montanum and Sedum album in response to water availability in the field. Oecologia 67:486–492
Evans JR, Caemmerer S von, Adams WW (eds) Ecology of photosynthesis in sun and shade. CSIRO, Melbourne
Feierabend J, Schaan C, Hertwig B (1992) Photoinactivation of catalase occurs under both high- and low-temperature stress conditions and accompanies photoinhibition of photosystem II. Plant Physiol 100:1554–1561
Griffiths H (1988) Crassulacean acid metabolism: a reappraisal of physiological plasticity in form and function. Adv Bot Res 15:43–92
Griffiths H, Ong BL, Avadhani PN, Goh CJ (1989) Recycling of respiratory CO2 during Crassulacean acid metabolism: alleviation of photoinhibition in Pyrrosia piloselloides. Planta 179: 115–122
Groenhof AC, Bryant JA, Etherington JR (1988) Photosynthetic changes in the inducible CAM plant Sedum telephium L. following the imposition of water stress. II. Changes in the activity of phosphoenolpyruvate carboxylase. Ann Bot 62:187–192
Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395
Irigoyen JJ, Emerich DW, Sánchez-Díaz M (1992) Alfalfa leaf senescence induced by drought stress: photosynthesis, hydrogen peroxide metabolism, lipid peroxidation and ethylene evolution. Physiol Plant 84:67–72
Lee HSJ, Griffiths H (1987) Induction and repression of CAM in Sedum telephium L. in response to photoperiod and water stress. J Exp Bot 38:834–841
Long SP, Humphries S, Falkowski PG (1994) Photoinhibition of photosynthesis in nature. Annu Rev Plant Physiol Plant Mol Biol 45:633–662
Martin CE, Higley M, Wang WZ (1988) Recycling of CO2 via Crassulacean acid metabolism in the rock outcrop succulent Sedum pulchellum Michx. (Crassulaceae). Photosynth Res 18:337–343
Powles SB (1984) Photoinhibition of photosynthesis induced by visible light. Annu Rev Plant Physiol 35:15–44
Salin ML (1988) Toxic oxygen species and protective systems of the chloroplast. Physiol Plant 72:681–689
Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
Sgherri CLM, Navari-Izzo F (1995) Sunflower seedlings subjected to increasing water deficit stress: oxidative stress and defense mechanisms. Physiol Plant 93:25–30
Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58
Smith TL, Eickmeier WG (1983) Limited photosynthetic plasticity in Sedum pulchellum Michx. Oecologia 56:374–380
Ting IP (1985) Crassulacean acid metabolism. Annu Rev Plant Physiol 36:595–622
Ting IP, Hann J, Holbrook NM, Putz FE, Sternberg L, Price D, Goldstein G (1987) Photosynthesis in hemiepiphytic species of Clusia and Ficus. Oecologia 74:339–346
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Castillo, F.J. Antioxidative protection in the inducible CAM plant Sedum album L. following the imposition of severe water stress and recovery. Oecologia 107, 469–477 (1996). https://doi.org/10.1007/BF00333937
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00333937