Summary
We have undertaken a systematic study to test the transformation of various species of gram-negative bacteria using the electroporation method. The data obtained show very clearly that a great variety of gram-negative bacteria — 15 different species belonging to 11 different genera — including freshly isolated wild-type strains can be transformed efficiently by use of the electric-field mediated transformation technique. These include species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, photosynthetic bacteria and strains for which transformation could not be achieved, up to now, by other methods.
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Communicated by J.W. Lengeler
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Wirth, R., Friesenegger, A. & Fiedler, S. Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation. Mol Gen Genet 216, 175–177 (1989). https://doi.org/10.1007/BF00332248
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DOI: https://doi.org/10.1007/BF00332248