Abstract
An enzyme with a particular 1,4-β-xylanase activity was identified and purified from wheat-bran culture medium of an Aspergillus awamori strain. With oligonucleotides based on the N-terminal amino-acid sequence of the enzyme, the exlA gene of A. awamori, encoding 1,4-β-xylanase A, has been cloned. Based on the deduced amino-acid sequence, 1,4-β-xylanase A is produced as a 211 amino-acid-residue-long precursor, which is converted post-translationally into a 184-aa-residue-long mature protein. Transformation of the original A. awamori strain with multiple copies of the exlA gene resulted in a 40-fold overproduction of 1,4-β-xylanase A. The overproduced enzyme has the same biochemical and enzymological properties as the wild-type enzyme.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amado R, Neukom H (1985) In: New approaches to research on cereal carbohydrates. Elsevier Science Publishers, Amsterdam, The Netherlands, p 241
Barbesgaard P (1977) In: Smith JE, Pateman JA (eds) Genetics and physiology of Aspergillus. Br Mycol Soc Symp Series no. 1. Academic Press, London, pp 391–404
Bennett JW (1985) In: Timberlake WE (ed) Molecular genetics of filamentous fungi. Alan R Liss, New York, pp 345–366
Bennett JW, Lasure LL (1991) In: Bennett JW, Lasure LL (eds) More gene manipulation in fungi. Academic Press, San Diego, California, pp 441–458
Biely P, Mislovicova D, Toman R (1985) Anal Biochem 144:142–146
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) J Mol Biol 170:827–842
Gorcom RFM van, Hessing JGM, Maat J, Roza M, Verbakel JMA (1991) PCT International Patent application, WO/91/19872
Gurr SJ, Unkles SE, Kinghorn JR (1988) In: Kinghorn JR (ed) Gene structure in eukaryotic microbes. SGM Spec Publ Vol 23. IRL Press, Oxford, pp 93–139
Hananan D (1983) J Mol Biol 166:557–580
Hartingsveldt W van, Mattern IE, van Zeijl CMJ, Pouwels PH, van den Hondel CAMJJ (1987) Mol Gen Genet 206:71–75
Heijne G von (1983) J Biochem 133:17–21
Heijne G von, Abrahmsen L (1989) FEBS Lett 244:439–446
Hondel CAMJJ van den, Punt PJ, van Gorcom RFM (1991) In: Benett JW, Lasur LL (eds) More gene manipulation in fungi. Academic Press, San Diego, California, pp 396–428
Ito K, Iwashita K, Iwano K (1992a) Biosci Biotech Biochem 56: 1338–1340
Ito K, Ogasawara H, Sugimoto T, Ishikawa T (1992b) Biosci Biotech Biochem 56:547–550
Kolar M, Punt PJ, van den Hondel CAMJJ, Schwab H (1988) Gene 13:137–144
Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene 56:117–124
Punt PJ, Dingemanse MA, Jacobs-Meijsing BJM, Pouwels PH, van den Hondel CAMJJ (1988) Gene 69:49–57
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Verdoes JC, Punt PJ, Schrickx JM, van Verseveld HM, Stouthamer AH, van den Hondel CAMJJ (1993) Transgenic Res 2:84–92
Wernars K (1986) DNA-mediated transformation of the filamentous fungus Aspergillus nidulans. Thesis, Agricultural University, Wageningen, The Netherlands
Wong KKY, Tan LUL, Saddler JN (1988) Microbiol Rev 52:305–317
Yanisch-Perron C, Vieira J, Messing J (1985) Gene 33:103–119
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hessing, J.G.M., van Rotterdam, C., Verbakel, J.M.A. et al. Isolation and characterization of a 1,4-β-endoxylanase gene of A. awamori . Curr Genet 26, 228–232 (1994). https://doi.org/10.1007/BF00309552
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00309552