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Increased ethanol production during growth of electric-field stimulated Kluyveromyces marxianus IMB3 during growth on lactose-containing media at 45°C

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Summary

Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional β-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

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Authors and Affiliations

  1. Biotechnology Research Group, School of Applied Biological and Chemical Sciences, University of Ulster, BT52 1SA, Coleraine, Co. Londonderry, Northern Ireland

    J. Simpson, D. Brady, A. Rollan, N. Barron, L. McHale & A. P. McHale

Authors
  1. J. Simpson
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  2. D. Brady
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  3. A. Rollan
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  4. N. Barron
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  5. L. McHale
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  6. A. P. McHale
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Simpson, J., Brady, D., Rollan, A. et al. Increased ethanol production during growth of electric-field stimulated Kluyveromyces marxianus IMB3 during growth on lactose-containing media at 45°C. Biotechnol Lett 17, 757–760 (1995). https://doi.org/10.1007/BF00130364

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  • DOI: https://doi.org/10.1007/BF00130364

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