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Recent Advances in Virus Diagnosis

A Seminar in the CEC Programme of Co-ordination of Research on Animal Pathology, held at the Veterinary Research Laboratories, Belfast, Northern Ireland, September 22–23, 1983

  • Book
  • © 1984

Overview

Editors:
  1. M. S. McNulty

    1. Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland

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  2. J. B. McFerran

    1. Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Current Topics in Veterinary Medicine (CTVM, volume 29)

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Table of contents (17 chapters)

  1. Front Matter

    Pages I-VIII
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  2. Viral Diagnosis by Immunofluorescence

    • P. S. Gardner
    Pages 1-14

  3. Applications of Immunofluorescence in Veterinary Viral Diagnosis

    • M. S. McNulty, G. M. Allan
    Pages 15-26

  4. Tagging of Viral Antigens using Immunoperoxidase and Immunogold Techniques

    • R. Ducatelle, F. Castryck, J. Hoorens
    Pages 27-36

  5. Detection of BVD Virus in Viremic Cattle by an Indirect Immunoperoxidase Technique

    • A. Meyling
    Pages 37-46

  6. Viral Diagnosis by Electron Microscopy

    • June D. Almeida
    Pages 47-59

  7. Capture Assays for the Detection of Virus-Specific IgM Antibody

    • Richard S. Tedder
    Pages 60-71

  8. Detection and Identification of Papillomaviruses in Benign and Malignant Tumours of Cattle

    • M. Saveria Campo
    Pages 72-94

  9. Detection of Viral Nucleic Acids in Cell Cultures and in Clinical Specimens by Spot Hybridization

    • T. Hyypia, P. Stalhandske, R. Vainionpaa, P. Halonen, U. Pettersson
    Pages 95-100

  10. Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA

    • A. L. J. Gielkens, J. T. van Oirschot, F. W. van Schie, B. Toma
    Pages 101-114

  11. Protein Blotting: The Basic Method and its Role in Viral Diagnosis

    • A. J. Herring, J. M. Sharp
    Pages 115-124

  12. The Use of Polyacrylamide Gel Electrophoresis of Virus RNA in the Study of Rotavirus Infections

    • D. Todd, M. S. McNulty, G. M. Allan
    Pages 125-135

  13. Examination of Rotavirus from Bovine and Porcine Species by Western Blotting

    • Anette G. Boetner, Jon Askaa
    Pages 136-144

  14. Use of Monoclonal Antibodies in Virus Diagnosis

    • D. van Zaane
    Pages 145-156

  15. Bovine Leukosis — Recent Developments with use of Monoclonal Antibodies and Elisa Tests

    • D. Portetelle, C. Bruck, M. Mammerickx, A. Burny
    Pages 157-170

  16. Radioimmunoassay

    • Daniel Levy
    Pages 171-185

  17. Immunoelectrophoretic Techniques

    • K. Dalsgaard
    Pages 186-194

  18. Diagnostic use of CNS Antibodies

    • Kurt Danner
    Pages 195-210

  19. Back Matter

    Pages 211-213
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Keywords

About this book

The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES .

Editors and Affiliations

  • Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland

    M. S. McNulty, J. B. McFerran

Bibliographic Information

  • Book Title: Recent Advances in Virus Diagnosis

  • Book Subtitle: A Seminar in the CEC Programme of Co-ordination of Research on Animal Pathology, held at the Veterinary Research Laboratories, Belfast, Northern Ireland, September 22–23, 1983

  • Editors: M. S. McNulty, J. B. McFerran

  • Series Title: Current Topics in Veterinary Medicine

  • DOI: https://doi.org/10.1007/978-94-009-6039-8

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: ECSC, EEC, EAEC, Brussels-Luxembourg 1984

  • Softcover ISBN: 978-94-009-6041-1Published: 14 October 2011

  • eBook ISBN: 978-94-009-6039-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: 222

  • Topics: Veterinary Medicine/Veterinary Science, Medical Microbiology

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