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Determination of Biocompatibility

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Book cover Biocompatibility of Dental Materials

Abstract

In the first part of this chapter principles and relevant test methods for testing of dental material biocompatibility are described, critically analyzed and related to the clinical relevance of the generated data. Prior to market launch, a great variety of different test methods may be applied to evaluate the risks of dental materials and to ensure material compatibility. Dentists need to be familiar with the principles and, in particular, with the problems of these test methods in order to critically challenge manufacturer claims and to independently advise the patient.

In the second part of the chapter, diagnostic methods used to evaluate patient reactions to dental materials (individual material compatibility; e.g. in cases of suspected allergy) are critically reviewed, including so-called holistic methods.

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References

  1. American Dental Association: ADA status report on the occurrence of galvanic corrosion in the mouth and its potential effects. J Am Dent Assoc 115, 783–787 (1987).

    Google Scholar 

  2. Arenholt-Bindslev, D., Bleeg, H.: Characterization of two types of human oral fibroblasts with a potential application to cellular toxicity studies: tooth pulp fibroblasts and buccal mucosa fibroblasts. Int Endod J 23, 84–91 (1990).

    Google Scholar 

  3. Ashby, J., Tennant, R.W.: Definitive relationship among chemical structure, carcinogenicity and mutagenicity for 301 chemicals tested by the US NTP. Mutat Res 257, 229–306 (1991).

    Google Scholar 

  4. Bachmann, A., Lutz, F.: Schmelzsprünge durch die Sensibilitätsprüfung mit CO2 Schnee und Dichlor-difluormethan – Eine vergleichende in vivo Untersuchung. [Cracks in the dental enamel caused by sensitivity testing with CO2 snow and dichloro-difluoromethane – a comparative in vivo study] Schweiz Mschr Zahnheilk 86, 1042–1059 (1976).

    Google Scholar 

  5. Banis, R.: Energetische Testverfahren und angewandte Kinesiologie – ein kritischer Überblick. [Energetic test methods and applied kinesiology – a critical review] Erfahrungsheilkunde 45, 245–250 (1996).

    Google Scholar 

  6. Basketter, D.A., Selbie, E., Scholes, E.W., Lees, D., Kimber, I., Botham, P.A.: Results with OECD recommended positive control sensitizer in the maximization, Buehler and local lymph node assays. Food Chem Toxicol 31, 63–67 (1993).

    Google Scholar 

  7. Bender, I.B., Freedland, J.B.: Clinical considerations in the diagnosis and treatment of intra-alveolar root fractures. J Am Dent Assoc 107, 595–600 (1983).

    Google Scholar 

  8. Beltrani, V. S., Bernstein, I. L., Cohen, D. E., Fonacier, L.: Contact dermatitis: a practice parameter. Ann Allergy Asthma Immunol 97 (3, suppl 2), 1–38 (2006).

    Google Scholar 

  9. Bieger, W.P.: Immuntoxikologie der Metalle. Labordiagnostik der Quecksilber- und Dentalmetall-Sensibilisierung. [Immunotoxicology of metals. Laboratory diagnosis of mercury and dental alloy sensitization] Clin Lab 42, 243–255 (1996).

    Google Scholar 

  10. Bourke, J., Coulson, I., English, J.: Guidelines for care of contact dermatitis. Br J Dermatol 145, 877–885 (2001).

    Google Scholar 

  11. Brannström, M., Mattson, B., Torstenson, B.: Material techniques for lining composite resin restorations: a critical approach. J Dent 19, 71–79 (1991).

    Google Scholar 

  12. Braun, A.-R., Frankenberger, R., Krämer, N.: Clinical performance and margin analysis of Ariston pHc versus Solitaire I as posterior restorations after 1 year. Clin Oral Investig 5, 139–147 (2001).

    Google Scholar 

  13. Brehler, R., Becker, D., Merk, H.: MELISA – In-vitro-Test zum Nachweis einer Kontaktallergie? Eine Stellungnahme der Deutschen Kontaktallergie-Gruppe. [In-vitro test for detection of a contact allergy? Statement of the German Group for Contact Allergy] Der Hautarzt 5, 418–419 (1998).

    Google Scholar 

  14. Bruze, M., Conde-Salazar, L., Goossens, A., Kanerva, L., White, I.R.: Thoughts on sensitizers in a standard patch test series. The European Society of Contact Dermatitis. Contact Dermatitis 41 (5), 241–250 (1999).

    Google Scholar 

  15. Casarett and Doull’s Toxicology: The Basic Science of Poisons, 3rd edn. McMillan, London 1986.

    Google Scholar 

  16. Cohen, D.E.: Contact dermatitis: a quarter-century perspective. J Am Acad Dermatol 51 (1), 60–61 (2004).

    Google Scholar 

  17. Cooley, R.L., Stilley, J., Lubow, R.M.: Evaluation of a digital pulp tester. Oral Surg 58, 437–442 (1984).

    Google Scholar 

  18. Deutsche Gesellschaft für Zahn-, Mund- und Kieferheilkunde: „Komplementäre Verfahren“ in der Zahnheilkunde. Stellungnahme der DGZMK. [The so-called Complementary Methods in dentistry. Statement of the German Scientific Society for Dentistry] Dtsch Zahnärztl Z 52, 564–566 (1997).

    Google Scholar 

  19. Donath, K.: The diagnostic value of the new method for the study of undecalcified bones and teeth with attached soft tissue [Säge-Schliff (sawing and grinding) technique]. Pathol Res Pract 179, 631–633 (1985).

    Google Scholar 

  20. Drews, M., Geurtsen, W.: Experimentell-klinische Überprüfung der Korrelation zwischen Mundstrommessungen und subjektiven Beschwerden. [Experimental and clinical evaluation of the correlation between oral current measurements and subjective symptoms] Dtsch Zahnärztl Z 48, 704–706 (1993).

    Google Scholar 

  21. Dumsha, T., Sydiskis, R.: Cytotoxicity testing of a dentin bonding system. Oral Surg Oral Med Oral Pathol 59, 637–641 (1985).

    Google Scholar 

  22. Eisenberg, D. M., Kessler, R.C., Foster, C., Norlock, F.E., Calkins, D.R., Delbanco, T.L.: Unconventional medicine in the United States – prevalence, costs, and patterns of use. N Engl J Med 328, 246–252 (1993).

    Google Scholar 

  23. Eisenberg, D. M., Davis, R. B., Ettner, S. L., Appel, S., Wilkey, S., Van Rompay, M., et al.: Trends in alternative medicine use in the United States, 1990-1997: results of a follow up national survey. J Am Dent Assoc 280, 1569–1575 (1998).

    Google Scholar 

  24. Elger, C.E.: Statement. In: Institut der Deutschen Zahnärzte (Hrsg.): Amalgam – Pro and Contra. Deutscher Ärzte-Verlag, Köln 1992, pp 169–172.

    Google Scholar 

  25. Federspiel, K., Herbst, V.: Handbuch: Die andere Medizin: “Alternative“ Heilmethoden für Sie bewertet. [Handbook on the Other Medicine: Alternative Treatment Methods Evaluated ] Stiftung Warentest in Zusammenarbeit mit dem Verein für Konsumenteninformation. Stiftung Warentest, Berlin 2005, p 333.

    Google Scholar 

  26. Fentem, J. H., Botham, P. A.: ECVAM’s activities in validating alternative tests for skin corrosion and irritation. Altern Lab Anim 30, 61–67 (2002).

    Google Scholar 

  27. Ferracane, J.L.: Elution of leachable components from composites. J Oral Rehabil 21, 441–452 (1994).

    Google Scholar 

  28. Fouad, A.F., Walton, R.E., Rittman, B.R.: Healing of induced periapical lesions in ferret canines. J Endod 19, 123–129 (1993).

    Google Scholar 

  29. Frankild, S., Volund, A., Wahlberg, J.E., Andersen, K.E.: Comparison of the sensitivities of the Buehler test and the guinea pig maximization test for predictive testing of contact allergy. Acta Derm Venereol 80, 256–262 (2000).

    Google Scholar 

  30. Friedl, K.-H., Schmalz, G., Hiller, K.-A., Shams, M.: Resin-modified glass ionomer cements: fluoride release and influence on Streptococcus mutans growth. Eur J Oral Sci 105, 81–85 (1997).

    Google Scholar 

  31. Fuss, Z., Trowbridge, H., Bender, I.B., Rickoff, B., Sorin, S.: Assessment of reliability of electrical and thermal pulp testing agents. J Endod 12, 301–305 (1986).

    Google Scholar 

  32. Garhammer, P., Schmalz, G., Hiller, K.-A., Reitinger, T., Stolz, W.: Patients with local adverse effects from dental alloys: frequency, complaints, symptoms, allergy. Clin Oral Investig 5, 240–249 (2001).

    Google Scholar 

  33. Garhammer, P., Hiller, K.-A., Reitinger, T., Schmalz, G.: Metal content of saliva of patients with and without metal restorations. Clin Oral Investig 8, 238–242 (2004).

    Google Scholar 

  34. Geurtsen, W.: Substances released from dental resin composites and glass ionomer cements. Eur J Oral Sci 106, 687–695 (1998).

    Google Scholar 

  35. Goodheart, G.: Applied kinesiology in dysfunction of the temporomandibular joint. Dent Clin North Am 27, 613–630 (1983).

    Google Scholar 

  36. Goodheart, G.: Applied kinesiology and dentistry. Basal Facts 9, 69–73 (1987).

    Google Scholar 

  37. Hamid, A., Hume, W.R.: Diffusion of resin monomers through human carious dentin in vitro. Endod Dent Traumatol 13, 1–5 (1997).

    Google Scholar 

  38. Hebling, J., Giro, E.M., Costa, C.A.: Biocompatibility of an adhesive system applied to the human dental pulp. J Endod 25, 676–682 (1999).

    Google Scholar 

  39. Heil, J., Reifferscheid, G., Waldmann, P., Leyhausen, G., Geurtsen, W.: Genotoxicity of dental material. Mutat Res 368, 181–194 (1996).

    Google Scholar 

  40. Hyman, J.J., Doblecki, W.: Computerized endodontic diagnosis. J Am Dent Assoc 107, 755–758 (1983).

    Google Scholar 

  41. Ingram, T.A., Peters, D.D.: Evaluation of the effects of carbon dioxide used as a pulpal test. Part 2. In vivo effect on canine enamel and pulpal tissues. J Endod 9, 296–303 (1983).

    Google Scholar 

  42. International Organization for Standardization: ISO 10993-3: Biological evaluation of medical devices. Part 3: Tests for genotoxicity, carcinogenicity and reproductive toxicity. International Organization for Standardization, Geneva 1992.

    Google Scholar 

  43. International Organization for Standardization: ISO 7405: Dentistry – preclinical evaluation of biocompatibility of medical devices used in dentistry. Test methods for dental materials. International Organization for Standardization, Geneva 2008.

    Google Scholar 

  44. Kallus, T., Mjör, I.A.: Incidence of adverse effects of dental materials. Scand J Dent Res 99, 236–240 (1991).

    Google Scholar 

  45. Kappert, H.F.: Oraler Galvanismus unter besonderer Berücksichtigung des Amalgams. [Oral galvanism related to amalgam] Phillip J 7, 233–240 (1990).

    Google Scholar 

  46. Kawahara, H., Shiota, M., Yamakawa, Y.: Studies on the effects of dental metals upon the mesenchymal cells in tissue culture. J Osaka Odontol Soc 18, 343–348 (1955).

    Google Scholar 

  47. Klötzer, W.T., Langeland, K.: Tierexperimentelle Prüfung von Materialien und Methoden der Kronen- und Brückenprothetik. [Testing of materials and methods for crown and bridge prosthesis on animals] Schweiz Monatsschr Zahnheilkd 83, 163–244 (1973).

    Google Scholar 

  48. Korting, H.C., Sterry, W.: Diagnostische Verfahren in der Dermatologie. [Diagnostic Methods in Dermatology] Blackwell Wissenschafts-Verlag, Berlin 1997.

    Google Scholar 

  49. Langer, H.: Das Schleimhautbrennen beim Tragen von Acrylatplatten. [Burning mucosa after insertion of acrylic plates] Dtsch Zahnärztl Z 11, 1321–1326 (1956).

    Google Scholar 

  50. Leyhausen, G., Heil, J., Reifferscheid, G., Waldmann, P., Geurtsen, W.: Genotoxicity and cytotoxicity of the epoxy resin-based root canal sealer AH plus. J Endod 25, 109–113 (1999).

    Google Scholar 

  51. Loftenius, A., Skoglund, A., Ekstrand, J., Hovmark, A., Möller, E.: No evidence for specific in vitro lymphocyte reactivity to HgCl2 in patients with dental amalgam related contact lesions. J Oral Pathol Med 28, 364–370 (1999).

    Google Scholar 

  52. Lutz, F., Mörmann, W., Lutz, T.: Schmelzsprünge durch die Vitalitätsprüfung mit Kohlensäureschnee? [Enamel cracks by carbon dioxide snow for pulp vitality testing] Schweiz Monatsschr Zahnheilk 84, 709–725 (1974).

    Google Scholar 

  53. Machtens, E.: Die zahnärztliche Behandlung von Patienten mit Herzschrittmachern. [Dental treatment of patients with cardiac pacemakers] Dtsch Zahnärztl Z 38, 1048–1052 (1983).

    Google Scholar 

  54. Macintyre, J.E.: Dictionary of Inorganic Compounds, vol. 1–7. Chapman & Hall, London 1992.

    Google Scholar 

  55. Magnusson, B., Kligman, A.M.: The identification of contact allergen by animal assay. The guinea pig maximization test. J Invest Dermatol 52, 268–276 (1969).

    Google Scholar 

  56. Maizumi, H., Sauerwein, E.: Die Wirkung verschiedener Vitalerhaltungs- und Wurzelfüllmittel auf Gewebekulturen. [Effect of various endodontic materials on cell cultures] Dtsch Zahnärztl Z 17, 1628–1634 (1962).

    Google Scholar 

  57. Maron, D.M., Ames, B.N.: Revised method for the Salmonella mutagenicity test. Mutat Res 113, 173–215 (1983).

    Google Scholar 

  58. Marquardt, H.: Lehrbuch der Toxikologie. [Textbook on Toxicology] Spektrum Akademischer Verlag, Heidelberg 1997.

    Google Scholar 

  59. Mastalier, O.: Ganzheitliche Zahn-, Mund- und Kieferheilkunde. Regulations- und Komplementärmethoden. [Holostic Dentistry. Regulation and Complementary Methods] Urban & Schwarzenberg, München 1995.

    Google Scholar 

  60. Matthews, B., Searle B.N.: Some observations on pulp testers. Br Dent J 137, 307–312 (1974).

    Google Scholar 

  61. McCann, J., Choi E., Yamasai E., Ames B.N.: Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci USA 72, 5135–5139 (1975).

    Google Scholar 

  62. Meiners, H.: Alternative Methoden in der Medizin. [Alternative methods in medicine] Dtsch Zahnärztl Z 52, 318–322 (1997).

    Google Scholar 

  63. Meiners, H.: Elektrische Erscheinungen an metallischen zahnärztlichen Restaurationen (oraler Galvinismus). [Electrical phenomena related to dental restorations (oral galvanism)] In: Institut der Deutschen Zahnärzte (Hrsg.): Amalgam im Spiegel kritischer Auseinandersetzungen. Interdisziplinäre Stellungnahmen zum „Kieler Amalgamgutachten“. Deutscher Ärzte-Verlag, Köln 1999, pp 57–62.

    Google Scholar 

  64. Meiners, H., Marxkors, R.: Bewertung elektrischer Vorgänge in der Mundhöhle. [Assessment of electrical processes in the oral cavity] In: Akademie Praxis und Wissenschaft in der DGZMK (Hrsg.): Pro und Contra – Alternative Heilmethoden in der Zahn-, Mund- und Kieferheilkunde. Hanser, München 1992, pp 71–81.

    Google Scholar 

  65. Meryon, S.D., Jakeman, K.J.: Uptake of zinc and fluoride by several dentin components. J Biomat Res 21, 127–135 (1987).

    Google Scholar 

  66. Meryon, S.D., Tobias, R.S., Johnson, S.G.: Penetration of dentin by different conditioners in vitro: a quantitative study. Endod Dent Traumatol 4, 118–121 (1988).

    Google Scholar 

  67. Mitchell, D.F., Shankwalker, G.B., Shazer, S.: Determining the tumorgenicity of dental materials. J Dent Res 39, 1023–1028 (1960).

    Google Scholar 

  68. Mjör, I.A.: Problems and benefits associated with restorative materials: side-effects and long-term cost. Adv Dent Res 6, 7–16 (1992).

    Google Scholar 

  69. Moharamzadeh, K., Brook, I. M., Van Noort, R., Scutt, A. M., Thornhill, M. H.: Tissue-engineered oral mucosa: a review of scientific literature. J Dent Res 86 (2), 115–124 (2007).

    Google Scholar 

  70. Moher, D., Schulz, K.F., Altmann, G.: The CONSORT statement: revised recommendations for improving the quality of reports of parallel-group randomised trials. Lancet 357, 1191–1194 (2001).

    Google Scholar 

  71. Montgomery, S., Ferguson, C.D.: Endodontics – diagnostics, treatment planning, and prognostic considerations. Dent Clin North Am 30, 533–548 (1986).

    Google Scholar 

  72. Murray, P. E., About, I., Lumley, P. J., Franquin, J. C., Remusat, M., Smith, A. J.: Cavity remaining dentin thickness and pulpal activity. Am J Dent 15, 41–46 (2002).

    Google Scholar 

  73. Nilner, K., Glantz, P.O., Zoger, B.: On interoral potential- and polarization-measurements of metallic restorations. Acta Odont Scand 40, 275–281 (1982).

    Google Scholar 

  74. Oepen, I., Federspiel, K., Sarma, A.: Lexikon der Parawissenschaften. [Enzyclopedia of Parasciences] Literaturverlag, Münster 1999.

    Google Scholar 

  75. Okamura, T., Morimoto, M., Yamane, G., Takahashi, S.: Langerhans’ cells in the murine oral mucosa in the inductive phase of delayed type hypersensitivity with 1-chloro-2,4-dinitrobenzene. Clin Exp Immunol 134, 188–194 (2003).

    Google Scholar 

  76. Organisation for Economic Co-operation and Development: Guidelines for the Testing of Chemicals, vol. 2. Organisation for Economic Co-operation and Development, Paris 1993.

    Google Scholar 

  77. Ostendorf, G.M.: Die Bedeutung von Naturheilverfahren und alternativen Methoden für die Zahnheilkunde. [The relevance of natural medicine and alternative methods for dentistry] Dtsch Zahnärztl Z 52, 329–331 (1997).

    Google Scholar 

  78. Pashley, D.H.: Smear layer: physiological considerations. Oper Dent 3, 513–529 (1984).

    Google Scholar 

  79. Pashley, D.H., Derkson, G.D., Tao, L., Derkson, M., Kalathoor, S.: The effects of a multi-step dentin bonding system on dentin permeability. Dent Mater 4, 60–63 (1988).

    Google Scholar 

  80. Peters, D.D., Mader, C.L., Donnelly, J.C.: Evaluation of the effects of carbon dioxide used as a pulpal test. 3. In vivo effect of human enamel. J Endod 12, 13–20 (1996).

    Google Scholar 

  81. Rechmann, P.: Nachweis metallischer Restaurationsmaterialien in klinisch unauffälliger Gingiva. [The detection of metallic restorative materials in clinically sound gingiva] Dtsch Zahnärztl Z 48, 270–275 (1993).

    Google Scholar 

  82. Schissl, M. J., Dodes, J. E.: Dentistry and alternative therapy. NY State Dent J 63, 32–37 (1997).

    Google Scholar 

  83. Schmalz, G.: Die Gewebeverträglichkeit zahnärztlicher Materialien – Möglichkeiten einer standardisierten Prüfung in der Zellkultur. [Biocompatibility of Dental Materials – Possibilities of a Standardized Test Using Cell Cultures] Thieme, Stuttgart 1981.

    Google Scholar 

  84. Schmalz, G.: Korrelationsanalysen zwischen Zellkulturen und Tierversuch. [Correlation analyses between cell cultures and animal experiments] Dtsch Zahnärztl Z 37, 184–186 (1982).

    Google Scholar 

  85. Schmalz, G.: The use of cell cultures for toxicity testing of dental materials – advantages and limitations. J Dent 22 (suppl. 2), 6–11 (1994).

    Google Scholar 

  86. Schmalz, G.: Concepts in biocompatibility testing of dental restorative materials. Clin Oral Investig 1, 154–162 (1997).

    Google Scholar 

  87. Schmalz, G.: The biocompatibility of nonamalgam dental filling materials. Eur J Oral Sci 106, 696–706 (1998).

    Google Scholar 

  88. Schmalz, G., Arenholt-Bindslev, D., Hiller, K.-A., Schweikl, H.: Epithelium-fibroblast co-culture for assessing the mucosal irritancy of metals used in dentistry. Eur J Oral Sci 105, 86–91 (1997).

    Google Scholar 

  89. Schmalz, G., Garhammer, P.: Biological interactions of dental cast alloys with oral tissues. Dent Mater 18, 396–406 (2002).

    Google Scholar 

  90. Schmalz, G., Garhammer, P., Hiller, K.-A., Reitinger, T.: Metal content of biopsies from the neighborhood of casting alloys. J Dent Res (abstract) 78, 236 (1999).

    Google Scholar 

  91. Schmalz, G., Hiller, K.-A., Garhammer, P., Reitinger, T.: Metal content of saliva from patients with and without metal restorations. J Dent Res (abstract) 80, 1254 (2001).

    Google Scholar 

  92. Schmalz, G., Hiller, K.-A., Nunez, L.J., Stoll, J., Weis, K.: Permeability characteristics of bovine and human dentin under different pretreatment conditions. J Endod 27, 23–30 (2001).

    Google Scholar 

  93. Schmalz, G., Schmalz, C., Rotgans, J.: Die Pulpaverträglichkeit eines Glasionomer- und Zinkphosphat-Zements. Dtsch Zahnärztl Z 41, 806–812 (1986).

    Google Scholar 

  94. Schmalz, G., Schuster, U., Nützel, K., Schweikl, H.: An in vitro pulp chamber with three-dimensional cell cultures. J Endod 25, 24–29 (1999).

    Google Scholar 

  95. Schmalz, G., Schweikl, H.: Characterization of an in vitro dentin barrier test using a standard toxicant. J Endod 20, 592–594 (1994).

    Google Scholar 

  96. Schmalz, G., Schweikl, H., Hiller, K.-A.: Release of prostaglandin E2, IL-6 and IL-8 from human oral epithelial culture models after exposure to compounds of dental materials. Eur J Oral Sci 108, 442–448 (2000).

    Google Scholar 

  97. Schriever, W., Diamond, L.E.: Electromotive forces and electric currents caused by metallic dental fillings. J Dent Res 31, 205–229 (1952).

    Google Scholar 

  98. Schuster, U., Schmalz, G., Thonemann, B., Mendel, N., Metzl, C.: Cytotoxicity testing with three-dimensional cultures of transfected pulp-derived cells. J Endod 27, 259–265 (2001).

    Google Scholar 

  99. Schweikl, H., Schmalz, G.: Triethylene glycol dimethacrylate induces large deletions in the hprt gene of V79 cells. Mutat Res 438, 71–78 (1999).

    Google Scholar 

  100. Schweikl, H., Schmalz, G., Federlin, M.: Mutagenicity of the root canal sealer AH Plus in the Ames test. Clin Oral Investig 2, 125–129 (1998).

    Google Scholar 

  101. Schweikl, H., Schmalz, G., Göttke, C.: The mutagenic activity of various dentin bonding agents. Biomaterials 17, 1451–1456 (1996).

    Google Scholar 

  102. Schweikl, H., Schmalz, G., Rackebrandt, K.: The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res 415, 119–130 (1998).

    Google Scholar 

  103. Schweikl, H., Schmalz, G., Stimmelmayr, H., Bey, B.: Mutagenicity of AH26 in an in vitro mammalian cell mutation assay. J Endod 21, 407–410 (1995).

    Google Scholar 

  104. Schweikl, H., Spagnuolo, G., Schmalz, G.: Genetic and cellular toxicology of dental resin monomers. J Dent Res 85, 870–877 (2006).

    Google Scholar 

  105. Schweikl, H., Hiller, K.-A., Eckhardt, A., Bolay, C., Spagnuolo, G., Stempfl, T., Schmalz, G.: Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate. Biomaterials 29,1377–1387 (2008).

    Google Scholar 

  106. Silverstone, L.M., Mjör, I.A.: Dental caries. In: Hørsted-Bindslev P., Mjör I. A. (eds): Modern Concepts in Operative Dentistry. Munksgaard, Copenhagen 1988, pp 16–58.

    Google Scholar 

  107. Staehle, H.J.: „Komplementäre Verfahren“ in der Zahnheilkunde. [Complementary methods in dentistry] Dtsch Zahnärztl Z 52, 323–328 (1997).

    Google Scholar 

  108. Staehle, H. J., Koch, M. J., Pioch, T.: Double-blind study on materials testing with applied kinesiology. J Dent Res 84, 1066–1069 (2005).

    Google Scholar 

  109. Svendsen, O., Garthoff, B., Spielmann, H., Hensten-Pettersen, A., Jensen, J.C., Kuijpers, M.R., Leimgruber, R., Liebsch, M., Müller-Lierheim, W.G.K., Rydhög, G., Sauer, U.G., Schmalz, G., Sim, B., Stea, S.: Alternatives to the animal testing of medical devices. ECVAM workshop report 17. ATLA 24, 659–669 (1996).

    Google Scholar 

  110. Tagger, M., Tagger, E.: Periapical reactions to calcium hydroxide-containing sealers and AH26 in monkeys. Endod Dent Traumatol 5, 139–146 (1989).

    Google Scholar 

  111. Takaku, S.: Studies on mercury concentration in saliva with particular reference to mercury dissolution from dental amalgam into saliva. Shikwa Dakuho 82, 385–406 (1982).

    Google Scholar 

  112. Teuber, S. S., Porch-Curren, C.: Unproved diagnostic and therapeutic approaches to food allergy and intolerance. Curr Opin Allergy Clin Immunol 3, 217–221 (2003).

    Google Scholar 

  113. Trowbridge, H.O.: Review of dental pain – histology and physiology. J Endod 12, 445–452 (1986).

    Google Scholar 

  114. Tziafas, D., Smith, A. J., Lesot, H.: Designing new treatment strategies in vital pulp therapy. J Dent 28, 77–92 (2000).

    Google Scholar 

  115. Vanherle, G.: Tandheelkundige verzorging met zilveramalgam. Ver K Acad Geneeskd Belg 58, 587–634 (1996).

    Google Scholar 

  116. Wirthlin, M.R., Armitage, G.C., Rao, S., Fritzinger, B., Phillips, S., Heller, J.: A mucosal irritancy test device for intraoral use in dogs. J Periodontol 68, 746–749 (1997).

    Google Scholar 

  117. Wirz, J.: Schädigung des Parodonts durch zahnärztliche Werkstoffe. [Damage of the periodontium by dental materials] Zahnärztl Welt/Reform 102, 146–162 (1993).

    Google Scholar 

  118. Wirz, J., Vock, M., Schmidli, F.: Splittertest – ein zuverlässiges Diagnosehilfsmittel bei Abklärungen von Metallunverträglichkeit. [The particle test – a reliable diagnostic tool for detection of a metal incompatibility] Quintessenz 47, 1373–1384 (1996).

    Google Scholar 

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Schmalz, G. (2009). Determination of Biocompatibility. In: Biocompatibility of Dental Materials. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77782-3_2

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