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Assays for GH, IGF-I, and IGF Binding Protein-3

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Book cover Hormone Assays in Biological Fluids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1065))

Abstract

The growth hormone/insulin-like growth factor (GH/IGF) axis is widely investigated in disorders of growth, development, and anterior pituitary function. Methods are described for three hormones in this axis (growth hormone, IGF-I, and IGF binding protein-3). Enzyme-linked immunosorbent assays (ELISAs) are described as they combine the specificity of antibody–antigen interactions with the sensitivity of simple enzyme assays. Many commercial immunoassays are available for the measurement of GH and IGF-I, and the main problems encountered in the measurement of these clinically important hormones are discussed.

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Author information

Authors and Affiliations

  1. Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, UK

    Nishan Guha

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  1. Nishan Guha
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Editor information

Editors and Affiliations

  1. Consultant Clinical Scientist The Old Dairy, Exmouth, United Kingdom

    Michael J. Wheeler

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Guha, N. (2013). Assays for GH, IGF-I, and IGF Binding Protein-3. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_7

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  • DOI: https://doi.org/10.1007/978-1-62703-616-0_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-615-3

  • Online ISBN: 978-1-62703-616-0

  • eBook Packages: Springer Protocols

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